2cc6227c-a416-4928-bd1f-8458741b8377
http://gcmd.nasa.gov/KeywordSearch/Metadata.do?Portal=GCMD&MetadataType=0&MetadataView=Full&KeywordPath=&EntryId=NSF-ANT06-32389
http://ipt.biodiversity.aq/resource.do?r=antarctic_peninsula_bacterioplankton_diversity
Antarctic Peninsula Bacterioplankton 16S rRNA gene surveys and metagenomes from Winter 2002 and Summer 2006.
Alison
Murray
DRI
Associate Research Professor
2215 Raggio Parkway
Reno
NV
89512
US
775 673 7361
Alison.Murray@dri.edu
http://www.dri.edu/alison-murray
Alison
Murray
DRI
Associate Research Professor
2215 Raggio Parkway
Reno
NV
89512
US
775 673 7361
Alison.Murray@dri.edu
http://www.dri.edu/alison-murray
Alison
Murray
DRI
Associate Research Professor
2215 Raggio Parkway
Reno
NV
89512
US
775 673 7361
Alison.Murray@dri.edu
http://www.dri.edu/alison-murray
principalInvestigator
Joseph
Grzymski
DRI
Assistant Research Professor
2215 Raggio Parkway
Reno
NV
89512
US
775 673 7478
Joe.Grzymski@dri.edu
http://www.dri.edu/joe-grzymski
principalInvestigator
2015-11-19
eng
Antarctic surface oceans are well-studied during summer when irradiance levels are high, sea ice is melting and primary productivity is at a maximum. Coincident with this timing, the bacterioplankton respond with significant increases in secondary productivity. Little is known about bacterioplankton in winter when darkness and sea-ice cover inhibit photoautotrophic primary production. We report here an environmental genomic and small subunit ribosomal RNA (SSU rRNA) analysis of winter and summer Antarctic Peninsula coastal seawater bacterioplankton. Intense inter-seasonal differences were reflected through shifts in community composition and functional capacities encoded in winter and summer environmental genomes with significantly higher phylogenetic and functional diversity in winter. In general, inferred metabolisms of summer bacterioplankton were characterized by chemoheterotrophy, photoheterotrophy and aerobic anoxygenic photosynthesis while the winter community included the capacity for bacterial and archaeal chemolithoautotrophy. Chemolithoautotrophic pathways were dominant in winter and were similar to those recently reported in global‘dark ocean’ mesopelagic waters. If chemolithoautotrophy is widespread in the Southern Ocean in winter, this process may be a previously unaccounted carbon sink and may help account for the unexplained anomalies in surface inorganic nitrogen content.
Bacterioplankton
Antarctic Peninsula
16S rRNA
metagenome
marine
archaea
bacteria
summer
winter
MARS
Other
GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml
This work is licensed under a Creative Commons CCZero 1.0 License http://creativecommons.org/publicdomain/zero/1.0/legalcode
http://genex2.dri.edu/index.php
Samples were collected in the nearshore region of Anvers Island, near Palmer Station.
-64.07
-64.05
-64.77
-64.78
2002-08-20
2002-08-20
2006-02-28
2006-02-28
2002-01-17
2002-01-17
2002-07-17
2002-07-17
Plankton surveys of community structure were conducted of those organisms passing through a 1.6 micron glass fiber filter.
domain
Archaea
domain
Bacteria
Alison
Murray
DRI
Associate Research Professor
2215 Raggio Parkway
Reno
NV
89512
US
775 673 7361
Alison.Murray@dri.edu
http://www.dri.edu/alison-murray
A MICROBIAL_SEQUENCE_SET Description file describing 9 data sets was uploaded to the IPT.
9 MIMARKS data files were uploaded to the IPT.
See Geographic coverage
Seawater was collected by submersible pump and filtered at the Station, see Grzymski et al. 2012 for details.
Sanger sequence data was automatically assembled and chimera checked; metagenome sequence data was automatically annotated at the Joint Genome Institute (see Grzymski et al. 2012).
IPY Antarctic Peninsula Bacterioplankton
Alison
Murray
principalInvestigator
NSF ANT 0632278 supported the researchers and Antarctic expeditions and DOE JGI Community Sequencing Program supported the metagenome and Sanger sequencing of 16S rRNA gene libraries; Pyrosequencing was provided by the International
Census of Marine Microbes (ICoMM) with financial support
from a W. M. Keck foundation award to the Marine Biological
Laboratory in Woods Hole.
Seawater (1.6 - 0.2 micron fraction) sampled off of Anvers Island, Antarctic Peninsula.
Samples were collected from surface waters on 12 occasions over the annual cycle in 2002 and in late February in 2006. The samples from 2002 were all analyzed by PCR-DGGE (Murray and Grzymski, 2007) and 3 samples were selected for Roche 454 tag sequencing targeting bacteria. In addition, the sample from 20 August 2002 was selected for (i) 16S rRNA gene clone library sequencing for bacteria and archaea; and (ii) for metagenome sequencing in which a large insert (40 kb) library was prepared and end sequences were determined for ~ 20K clones. Selected clones (~ 96) were then fully sequenced.
In 2006 samples were collected at the end of February, in which we selected a sample from 28 February 2006 for 16S rRNA gene clone library sequencing for bacteria and archaea; in addition a metagenome sequencing effort was conducted in which a large insert (40 kb) library was prepared and end sequences were determined for ~ 20K clones. Selected clones (~ 96) were then fully sequenced.
2012-05-10T03:21:12.833+02:00
dataset
Murray, AE and JJ Grzymski. 2007. Diversity and genomics of Antarctic marine microorganisms. Phil. Trans. Roy. Soc. Ser. B. 362:2259-2271.
Grzymski, JJ, CS Riesenfeld , TJ Williams, AM Dussaq, H Ducklow, M Erickson, R Cavicchioli, & AE Murray. 2012. A metagenomic assessment of winter and summer bacterioplankton from Antarctic Peninsula coastal surface waters. ISME Journal.
Ghiglione, JF, and AE Murray. 2012. Pronounced summer to winter differences and higher wintertime richness in coastal Antarctic marine bacterioplankton. Environ. Microbiol. 14(3): 617-629.
Williams, TJ, E Long, F Evans, MZ DeMaere, FM Lauro, MJ Raftery, H Ducklow, JJ Grzymski, AE Murray, R Cavicchioli. 2012. A metaproteomic assessment of summer and winter bacterioplankton from Antarctic Peninsula coastal surface waters. ISME Journal 6:1883-1900.
http://ipt.biodiversity.aq/logo.do?r=antarctic_peninsula_bacterioplankton_diversity
2cc6227c-a416-4928-bd1f-8458741b8377/v6.xml