2cc6227c-a416-4928-bd1f-8458741b8377 http://gcmd.nasa.gov/KeywordSearch/Metadata.do?Portal=GCMD&MetadataType=0&MetadataView=Full&KeywordPath=&EntryId=NSF-ANT06-32389 http://ipt.biodiversity.aq/resource.do?r=antarctic_peninsula_bacterioplankton_diversity Antarctic Peninsula Bacterioplankton 16S rRNA gene surveys and metagenomes from Winter 2002 and Summer 2006. Alison Murray DRI Associate Research Professor
2215 Raggio Parkway Reno NV 89512 US
775 673 7361 Alison.Murray@dri.edu http://www.dri.edu/alison-murray
Alison Murray DRI Associate Research Professor
2215 Raggio Parkway Reno NV 89512 US
775 673 7361 Alison.Murray@dri.edu http://www.dri.edu/alison-murray
Alison Murray DRI Associate Research Professor
2215 Raggio Parkway Reno NV 89512 US
775 673 7361 Alison.Murray@dri.edu http://www.dri.edu/alison-murray principalInvestigator
Joseph Grzymski DRI Assistant Research Professor
2215 Raggio Parkway Reno NV 89512 US
775 673 7478 Joe.Grzymski@dri.edu http://www.dri.edu/joe-grzymski principalInvestigator
2015-11-19 eng Antarctic surface oceans are well-studied during summer when irradiance levels are high, sea ice is melting and primary productivity is at a maximum. Coincident with this timing, the bacterioplankton respond with significant increases in secondary productivity. Little is known about bacterioplankton in winter when darkness and sea-ice cover inhibit photoautotrophic primary production. We report here an environmental genomic and small subunit ribosomal RNA (SSU rRNA) analysis of winter and summer Antarctic Peninsula coastal seawater bacterioplankton. Intense inter-seasonal differences were reflected through shifts in community composition and functional capacities encoded in winter and summer environmental genomes with significantly higher phylogenetic and functional diversity in winter. In general, inferred metabolisms of summer bacterioplankton were characterized by chemoheterotrophy, photoheterotrophy and aerobic anoxygenic photosynthesis while the winter community included the capacity for bacterial and archaeal chemolithoautotrophy. Chemolithoautotrophic pathways were dominant in winter and were similar to those recently reported in global‘dark ocean’ mesopelagic waters. If chemolithoautotrophy is widespread in the Southern Ocean in winter, this process may be a previously unaccounted carbon sink and may help account for the unexplained anomalies in surface inorganic nitrogen content. Bacterioplankton Antarctic Peninsula 16S rRNA metagenome marine archaea bacteria summer winter MARS Other GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml This work is licensed under a Creative Commons CCZero 1.0 License http://creativecommons.org/publicdomain/zero/1.0/legalcode http://genex2.dri.edu/index.php Samples were collected in the nearshore region of Anvers Island, near Palmer Station. -64.07 -64.05 -64.77 -64.78 2002-08-20 2002-08-20 2006-02-28 2006-02-28 2002-01-17 2002-01-17 2002-07-17 2002-07-17 Plankton surveys of community structure were conducted of those organisms passing through a 1.6 micron glass fiber filter. domain Archaea domain Bacteria Alison Murray DRI Associate Research Professor
2215 Raggio Parkway Reno NV 89512 US
775 673 7361 Alison.Murray@dri.edu http://www.dri.edu/alison-murray
A MICROBIAL_SEQUENCE_SET Description file describing 9 data sets was uploaded to the IPT. 9 MIMARKS data files were uploaded to the IPT. See Geographic coverage Seawater was collected by submersible pump and filtered at the Station, see Grzymski et al. 2012 for details. Sanger sequence data was automatically assembled and chimera checked; metagenome sequence data was automatically annotated at the Joint Genome Institute (see Grzymski et al. 2012). IPY Antarctic Peninsula Bacterioplankton Alison Murray principalInvestigator NSF ANT 0632278 supported the researchers and Antarctic expeditions and DOE JGI Community Sequencing Program supported the metagenome and Sanger sequencing of 16S rRNA gene libraries; Pyrosequencing was provided by the International Census of Marine Microbes (ICoMM) with financial support from a W. M. Keck foundation award to the Marine Biological Laboratory in Woods Hole. Seawater (1.6 - 0.2 micron fraction) sampled off of Anvers Island, Antarctic Peninsula. Samples were collected from surface waters on 12 occasions over the annual cycle in 2002 and in late February in 2006. The samples from 2002 were all analyzed by PCR-DGGE (Murray and Grzymski, 2007) and 3 samples were selected for Roche 454 tag sequencing targeting bacteria. In addition, the sample from 20 August 2002 was selected for (i) 16S rRNA gene clone library sequencing for bacteria and archaea; and (ii) for metagenome sequencing in which a large insert (40 kb) library was prepared and end sequences were determined for ~ 20K clones. Selected clones (~ 96) were then fully sequenced. In 2006 samples were collected at the end of February, in which we selected a sample from 28 February 2006 for 16S rRNA gene clone library sequencing for bacteria and archaea; in addition a metagenome sequencing effort was conducted in which a large insert (40 kb) library was prepared and end sequences were determined for ~ 20K clones. Selected clones (~ 96) were then fully sequenced.
2012-05-10T03:21:12.833+02:00 dataset Murray, AE and JJ Grzymski. 2007. Diversity and genomics of Antarctic marine microorganisms. Phil. Trans. Roy. Soc. Ser. B. 362:2259-2271. Grzymski, JJ, CS Riesenfeld , TJ Williams, AM Dussaq, H Ducklow, M Erickson, R Cavicchioli, & AE Murray. 2012. A metagenomic assessment of winter and summer bacterioplankton from Antarctic Peninsula coastal surface waters. ISME Journal. Ghiglione, JF, and AE Murray. 2012. Pronounced summer to winter differences and higher wintertime richness in coastal Antarctic marine bacterioplankton. Environ. Microbiol. 14(3): 617-629. Williams, TJ, E Long, F Evans, MZ DeMaere, FM Lauro, MJ Raftery, H Ducklow, JJ Grzymski, AE Murray, R Cavicchioli. 2012. A metaproteomic assessment of summer and winter bacterioplankton from Antarctic Peninsula coastal surface waters. ISME Journal 6:1883-1900. http://ipt.biodiversity.aq/logo.do?r=antarctic_peninsula_bacterioplankton_diversity 2cc6227c-a416-4928-bd1f-8458741b8377/v6.xml