http://ipt.biodiversity.aq/resource?r=cyano_16s cyanobacterial mats of from the Byers Peninsula, Antarctica Julia Kleinteich University of Liège Researcher
Liège Lieège BE
j.kleinteich@ulg.ac.be
Julien Cigar jcigar@ulb.ac.be 2017-08-18 eng Cyanobacterial 16S rRNA gene sequences from cyanobacterial mats of Antarctic (Byers Peninsula) origin obtained by clone library Metadata GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License. Byers Peninsula, Livingston Island, Antarctica -61.5 -61 -62.5 -62.7 2009-02-01 2009-02-28 All data provided are OTUs of the 16S rRNA gene which were identified via a comparison to online databases to family or genus level. For most OTUs identification to species level was not possible phylum Cyanobacteria Cyanobacteria unkown Julia Kleinteich University of Liège Researcher
Liège Lieège BE
j.kleinteich@ulg.ac.be
Daniel R Dietrich University of Konstanz Researcher
Konztanz DE
After collection the samples were stored at -20°C until further processing. DNA was extracted from the samples in three replicates and combined. 16S rRNA genes were amplified using cyanobacteria specific primers (Saker et al. 2005). PCR Products were cloned using the TOPO TA Cloning Kit following the standard protocol. Two to three clones of each individual restriction fragment length polymorphism pattern were sequenced at GATC Biotech. All sequences were deposited with the GenBank database. Saker, M. L., Jungblut, A. D., Neilan, B. A., Rawn, D. F. K. & Vasconcelos, V. M. Detection of microcystin synthetase genes in health food supplements containing the freshwater cyanobacterium Aphanizomenon flos-aquae. Toxicon 46, 555?562 (2005). Samples were collected on Byers Peninsula (ASPA 126) in February 2009. This area is a summer snow- and ice-free area. Various microbial mats on wet soil, meltwater and seepages were sampled. Cyanobacterial samples were collected from five different sampling sites in the Antarctic Specially Protected Area (ASPA) No. 126 of Byers Peninsula, Livingston Island (62° 34' 35" to 62° 4' 35" S and 60° 54' 14" to 61° 13' 07" W), South Shetland Islands, Antarctic Peninsula, during an expedition in February 2009. Microbial mats were probed using a sterile spatula, sealed in sterile plastic bags or tubes and stored frozen (-20 °C) for DNA extraction. Data were published in a peer-reviewed journal DOI: 10.1038/NCLIMATE1418 DI698/18-1 Dietrich Daniel R Dietrich principalInvestigator Deutsche Forschungsgesellschaft DFG
2017-06-20T02:28:44.641+02:00 dataset http://ipt.biodiversity.aq/resource?id=cyano_16s/v3.1.xml