http://ipt.biodiversity.aq/resource?r=cyano_16s
cyanobacterial mats of from the Byers Peninsula, Antarctica
Julia
Kleinteich
University of Liège
Researcher
Liège
Lieège
BE
j.kleinteich@ulg.ac.be
Julien
Cigar
jcigar@ulb.ac.be
2017-08-18
eng
Cyanobacterial 16S rRNA gene sequences from cyanobacterial mats of Antarctic (Byers Peninsula) origin obtained by clone library
Metadata
GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml
This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
Byers Peninsula, Livingston Island, Antarctica
-61.5
-61
-62.5
-62.7
2009-02-01
2009-02-28
All data provided are OTUs of the 16S rRNA gene which were identified via a comparison to online databases to family or genus level. For most OTUs identification to species level was not possible
phylum
Cyanobacteria
Cyanobacteria
unkown
Julia
Kleinteich
University of Liège
Researcher
Liège
Lieège
BE
j.kleinteich@ulg.ac.be
Daniel R
Dietrich
University of Konstanz
Researcher
Konztanz
DE
After collection the samples were stored at -20°C until further processing. DNA was extracted from the samples in three replicates and combined. 16S rRNA genes were amplified using cyanobacteria specific primers (Saker et al. 2005). PCR Products were cloned using the TOPO TA Cloning Kit following the standard protocol.
Two to three clones of each individual restriction fragment length polymorphism pattern were sequenced at GATC Biotech. All sequences were deposited with the GenBank database.
Saker, M. L., Jungblut, A. D., Neilan, B. A., Rawn, D. F. K. & Vasconcelos, V. M. Detection of microcystin synthetase genes in health food supplements containing the freshwater cyanobacterium Aphanizomenon flos-aquae. Toxicon 46, 555?562 (2005).
Samples were collected on Byers Peninsula (ASPA 126) in February 2009. This area is a summer snow- and ice-free area. Various microbial mats on wet soil, meltwater and seepages were sampled.
Cyanobacterial samples were collected from five
different sampling sites in the Antarctic Specially Protected Area
(ASPA) No. 126 of Byers Peninsula, Livingston Island (62° 34' 35" to 62° 4' 35" S and 60° 54' 14" to 61° 13' 07" W), South Shetland Islands, Antarctic Peninsula, during an expedition in February 2009.
Microbial mats were probed using a sterile spatula, sealed in sterile plastic bags or tubes and stored frozen (-20 °C) for DNA extraction.
Data were published in a peer-reviewed journal DOI: 10.1038/NCLIMATE1418
DI698/18-1 Dietrich
Daniel R
Dietrich
principalInvestigator
Deutsche Forschungsgesellschaft DFG
2017-06-20T02:28:44.641+02:00
dataset
http://ipt.biodiversity.aq/resource?id=cyano_16s/v3.1.xml