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Marine bacterioplankton community structure in the vicinity of Antarctic icebergs

Latest version published by SCAR - Microbial Antarctic Resource System on Aug 14, 2014 SCAR - Microbial Antarctic Resource System

We studied marine bacterioplankton in the Scotia Sea in June 2008 and in the northwest Weddell Sea in March to mid April 2009 in waters proximal to three free-drifting icebergs (SS-1, A-43k, and C-18a), in a region with a high density of smaller icebergs (iceberg alley), and at stations that were upstream of the iceberg trajectories designated as far-field reference sites that were between 16-75 km away. Hydrographic parameters were used to define water masses in which comparisons between bacterioplankton-associated characteristics within and between water masses could be made. Influences of the icebergs on early winter Scotia Sea bacterioplankton were minimal, if not deleterious, as we found lower levels of heterotrophic production near A-43k in comparison to stations > 16 km away. Small but significant differences in bacterioplankton community structure were observed between two icebergs studied in early winter Scotia Sea. These icebergs differed greatly in size and the findings suggest that the larger iceberg had a greater effect. In the NW Weddell Sea in March–mid April there were some significant differences in community structure in the winter water and underlying upper circumpolar deep water masses between stations occupied close to C-18a and at stations 18 km away (i.e. Polaribacter and Pelagibacter-related 16S rRNA gene fragments were at low levels at the 18 km stations), though higher resolution, high throughput profiling tools will be needed to pinpoint specific organisms and ecological types. Likewise, a better understanding of local to regional scale structure of bacterioplankton communities is necessary. Overall, the results show that bacterioplankton, dominated by Rhodobacteracae Pelagibacter, and uncultivated Gammaproteobacteria groups were minimally influenced by icebergs in the regions and seasons studied here – at least directly – though further work addressing different scales, sizes of icebergs, and seasons is needed to better understand bacterioplankton-associated ecological processes and carbon cycling in regions of high iceberg production.

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How to cite

Researchers should cite this work as follows:

Murray, AE, V Peng, C Tyler, P Wagh. 2011. Marine bacterioplankton biomass, activity and community structure in the vicinity of Antarctic icebergs. Deep Sea Research, II. 58: 1407-1421.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 2ae9d4b7-012b-4649-862f-1c3c6c5f9e5f.  SCAR - Microbial Antarctic Resource System publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Scientific Committee on Antarctic Research.

Keywords

Bacterioplankton; marine bacteria; 16S rRNA; LHPCR; Southern Ocean; icebergs; productivity; aminopeptidase activity; Weddell Sea; Heterotrophy; Other

External data

The resource data is also available in other formats

Iceberg Project Websitehttp://iceberg.dri.edu/ UTF-8 text

Contacts

Who created the resource:

Alison Murray
Research Professor
Desert Research Institute 2215 Raggio Parkway 89512 Reno CA US 775-673-7361
http://www.dri.edu/alison-murray

Who can answer questions about the resource:

Alison Murray
Research Professor
Desert Research Institute 2215 Raggio Parkway 89512 Reno CA US 775-673-7361
http://www.dri.edu/alison-murray

Who filled in the metadata:

Alison Murray
Research Professor
Desert Research Institute 2215 Raggio Parkway 89512 Reno CA US 775-673-7361
http://www.dri.edu/alison-murray

Who else was associated with the resource:

Principal Investigator
Alison Murray
Research Professor
Desert Research Institute 2215 Raggio Parkway 89512 Reno CA US 775-673-7361
http://www.dri.edu/alison-murray

Geographic Coverage

This study occurred over two cruises in 2008 in the Scotia Sea, and in 2009 in the NW Weddell Sea, thus the bounding box encompasses the coverage in both cruises.

Bounding Coordinates South West [-62, -51], North East [-57, -42]

Taxonomic Coverage

Plankton surveys of community structure were conducted on bacteria passing through a 3.0 micron membrane filter.

Domain  Bacteria

Temporal Coverage

Start Date / End Date 2008-06-07 / 2008-06-23
Start Date / End Date 2014-03-10 / 2014-04-07

Project Data

No Description available

Title Marine bacterioplankton community structure in the vicinity of Antarctic icebergs
Funding This research was supported by NSF award ANT-0636543.
Study Area Description Hydrological casts of the water column were collected to 500 m using a Conductivity-Temperature-Depth (CTD) rosette in areas surrounding three icebergs, SS-1, and A-43k in the Scotia Sea north of the South Shetland Islands, and C-18a during March to mid April 2009 in the Powell Basin region in the NW Weddell Sea.

The personnel involved in the project:

Principal Investigator
Alison Murray

Sampling Methods

Samples were collected at 8 depths between 10-500 m with a CTD rosette and Niskin bottles and a tow fish for selected surface samples. Seawater for molecular analyses was filtered first through an in-line 3.0 micron filter and the < 3.0 micron fraction was collected onto a 0.2 micron filter.

Study Extent See Geographic Coverage
Quality Control Sanger sequence data was assembled and manually curated using Sequencher and chimera checked using Mallard.

Method step description:

  1. Three bacterial 16S rRNA gene clone libraries were prepared for this data set. A MICROBIAL_SEQUENCE_SET Description file describing 3 data sets was uploaded to the IPT. MIMARKS data for these three samples were uploaded to the IPT.

Collection Data

Collection Name Bacterioplankton around Antarctic Icebergs
Collection Identifier IB02 and IB03
Parent Collection Identifier Not applicable
Specimen preservation methods Deep frozen

Bibliographic Citations

  1. Vernet, M., KL Smith Jr., AO Cefarelli, JJ Helly, RS Kaufmann, H Lin, DG Long, AE Murray, BH Robison, HA Ruhl, TJ Shaw, AD Sherman, J Sprintall, GR Stephenson Jr., KM Stuart, BS Twining. 2012. Islands of ice: Influence of free-drifting Antarctic icebergs on pelagic marine ecosystems. Oceangr. 25(3): 38-39. http://dx.doi.org/10.5670/oceanog.2012.72