Microbiome (Archaea, Bacteria and Eukaryota) of lake Fryxell and lake Bonney (McMurdo Dry Valleys, Antarctica)

Latest version published by SCAR - Microbial Antarctic Resource System on Mar 19, 2019 SCAR - Microbial Antarctic Resource System
Publication date:
19 March 2019
License:
CC-BY 4.0

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Description

Amplicon sequencing dataset (454 pyrosequencing) of Archaea (16S), Bacteria (16S) and Eukaryote (18S) in the water column of lake Fryxell and Lake Bonney, in the McMurdo Dry valleys (Antarctica).

Versions

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How to cite

Researchers should cite this work as follows:

Vick-Majors T, Priscu J, Amaral-Zettler L (2019): Microbiome (Archaea, Bacteria and Eukaryota) of lake Fryxell and lake Bonney (McMurdo Dry Valleys, Antarctica). v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbiome_mc_murdo_dry_valley_lakes_antarctica&v=1.2

Rights

Researchers should respect the following rights statement:

The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 4b44031f-2271-48a8-b3d9-43b90768e071.  SCAR - Microbial Antarctic Resource System publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Scientific Committee on Antarctic Research.

Keywords

Metadata

Contacts

Trista Vick-Majors
  • Originator
  • Point Of Contact
Montana State University
Bozeman
US
John Priscu
  • Originator
Montana State University
Bozeman
US
Linda Amaral-Zettler
  • Originator
he Josephine Bay Paul Center for Comparative Molecular Biology and Evolution
Woods Hole
US
Maxime Sweetlove
  • Metadata Provider
Research assistent
Royal Belgian Institute of Natural Sciences
Rue Vautier 29
1000 Brussels
BE

Geographic Coverage

Lake Fryxell and lake Bonney, McMurdo Dry Valleys, Antarctica

Bounding Coordinates South West [-77.72, 162.297], North East [-77.72, 163.146]

Taxonomic Coverage

Archaea were profiled by targeting the v6 region of the 16S ssu rRNA gene

Domain Archaea (Archaea)

Bacteria were profiled by targeting the v6 region of the 16S ssu rRNA gene

Domain Bacteria (Bacteria)

Eukaryota were profiled by targeting the v9 region of the 18S ssu rRNA gene

Domain Eukaryota (Eukaryotes)

Temporal Coverage

Start Date / End Date 2007-11-22 / 2008-03-25

Project Data

No Description available

Title MIRADA-LTERS
Funding Funding was provided by NSF DEB-0717390 (MIRADA-LTERS) and OPP-1115254, OPP-0838953, OPP-1027284 and OPP- 0839075. The Montana Space Grant Consortium provided additional funding.

The personnel involved in the project:

Trista Vick-Majors

Sampling Methods

Duplicate samples were collected at depths of 6 m (bacterial and primary production maximum) and 9 m (chemocline; chlorophyll-a maximum) in lake Fryxell (2 November 2007 and 25 March 2008) and 13 m (chemocline; bacterial production, chlorophyll-a, primary production maximum) and 18 m (hypersaline, bottom of trophogenic zone) in lake Bonney (west Lobe; 30 November 2007 and 12 March 2008). Samples for DNA extraction were filtered onto 0.2-μM Sterivex filters (Millipore, Billerica, MA, USA) and stored with 2.0 ml of Puregene lysis buffer at −20 °C until further processing.

Study Extent Sampling occurred during the Austral summer and autumn in lake Fryxell and lake Bonney, Antarctica

Method step description:

  1. DNA extraction protocols can be found at http://amarallab.mbl.edu.
  2. The V6 hypervariable regions of Archaa and bacteria were amplified, while for Eukaryota, amplification of the V9 hypervariable region followed established protocols (Amaral-Zettler et al., 2009). We multiplex-sequenced the resulting amplicons using bar-coded primers (Huber et al., 2007; Amaral-Zettler et al., 2009) on a 454 Genome Sequencer FLX (Roche, Switzerland) using the manufacturer’s recommended protocol.

Bibliographic Citations

  1. Vick-Majors, T. J., Priscu, J. C., & Amaral-Zettler, L. A. (2014). Modular community structure suggests metabolic plasticity during the transition to polar night in ice-covered Antarctic lakes. The ISME journal, 8(4), 778.
  2. Xu, Y., Vick-Majors, T., Morgan-Kiss, R., Priscu, J. C., & Amaral-Zettler, L. (2014). Ciliate diversity, community structure, and novel taxa in lakes of the McMurdo Dry Valleys, Antarctica. The Biological Bulletin, 227(2), 175-190.

Additional Metadata

Alternative Identifiers 4b44031f-2271-48a8-b3d9-43b90768e071
https://ipt.biodiversity.aq/resource?r=microbiome_mc_murdo_dry_valley_lakes_antarctica