cyanobacterial mats of from the Byers Peninsula, Antarctica

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Latest version published by SCAR - Microbial Antarctic Resource System on Mar 19, 2019 SCAR - Microbial Antarctic Resource System
Publication date:
19 March 2019
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CC-BY 4.0

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Description

Cyanobacterial 16S rRNA gene sequences from cyanobacterial mats of Antarctic (Byers Peninsula) origin obtained by clone library

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The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

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Keywords

Metadata

Contacts

Julia Kleinteich
  • Originator ●
  • Point Of Contact
Researcher
University of Liège
Liège
Lieège
BE
Julien Cigar
  • Metadata Provider
Daniel R Dietrich
  • Point Of Contact
Researcher
University of Konstanz
Konztanz
DE

Geographic Coverage

Byers Peninsula, Livingston Island, Antarctica

Bounding Coordinates South West [-62.7, -61.5], North East [-62.5, -61]

Taxonomic Coverage

All data provided are OTUs of the 16S rRNA gene which were identified via a comparison to online databases to family or genus level. For most OTUs identification to species level was not possible

Phylum Cyanobacteria (Cyanobacteria)

Temporal Coverage

Start Date / End Date 2009-02-01 / 2009-02-28

Project Data

No Description available

Title DI698/18-1 Dietrich
Funding Deutsche Forschungsgesellschaft DFG

The personnel involved in the project:

Daniel R Dietrich
  • Principal Investigator

Sampling Methods

Cyanobacterial samples were collected from five different sampling sites in the Antarctic Specially Protected Area (ASPA) No. 126 of Byers Peninsula, Livingston Island (62° 34' 35" to 62° 4' 35" S and 60° 54' 14" to 61° 13' 07" W), South Shetland Islands, Antarctic Peninsula, during an expedition in February 2009. Microbial mats were probed using a sterile spatula, sealed in sterile plastic bags or tubes and stored frozen (-20 °C) for DNA extraction.

Study Extent Samples were collected on Byers Peninsula (ASPA 126) in February 2009. This area is a summer snow- and ice-free area. Various microbial mats on wet soil, meltwater and seepages were sampled.
Quality Control Data were published in a peer-reviewed journal DOI: 10.1038/NCLIMATE1418

Method step description:

  1. After collection the samples were stored at -20°C until further processing. DNA was extracted from the samples in three replicates and combined. 16S rRNA genes were amplified using cyanobacteria specific primers (Saker et al. 2005). PCR Products were cloned using the TOPO TA Cloning Kit following the standard protocol. Two to three clones of each individual restriction fragment length polymorphism pattern were sequenced at GATC Biotech. All sequences were deposited with the GenBank database. Saker, M. L., Jungblut, A. D., Neilan, B. A., Rawn, D. F. K. & Vasconcelos, V. M. Detection of microcystin synthetase genes in health food supplements containing the freshwater cyanobacterium Aphanizomenon flos-aquae. Toxicon 46, 555?562 (2005).

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