cyanobacterial mats of from the Byers Peninsula, Antarctica

Julia Kleinteich1, Julien Cigar2

1 University of Liège, Liège, Belgium; 2 

Corresponding author(s): Julia Kleinteich (j.kleinteich@ulg.ac.be), Julien Cigar (jcigar@ulb.ac.be)

Received {date}; Revised {date}; Accepted {date}; Published {date}

Citation: Combination of authors, year of data paper publication (in parentheses), Title, Journal Name, Volume, Issue number (in parentheses), and doi of the data paper.

Abstract

Cyanobacterial 16S rRNA gene sequences from cyanobacterial mats of Antarctic (Byers Peninsula) origin obtained by clone library

Keywords: Metadata

Project details

Project title: DI698/18-1 Dietrich
Personnel: Daniel R  Dietrich
Funding: Deutsche Forschungsgesellschaft DFG

Data published through GBIF: http://ipt.biodiversity.aq/resource?r=cyano_16s

Taxonomic coverage

General taxonomic coverage description: All data provided are OTUs of the 16S rRNA gene which were identified via a comparison to online databases to family or genus level. For most OTUs identification to species level was not possible
Taxonomic ranks
Phylum: Cyanobacteria
Common names: Cyanobacteria

Spatial coverage

General spatial coverage: Byers Peninsula, Livingston Island, Antarctica
Coordinates: 62°42'0''S and 62°30'0''S Latitude; 61°30'0''W and 61°0'0''W Longitude 

Temporal coverage: February 1, 2009 - February 28, 2009

Methods

Method step description: After collection the samples were stored at -20°C until further processing. DNA was extracted from the samples in three replicates and combined. 16S rRNA genes were amplified using cyanobacteria specific primers (Saker et al. 2005). PCR Products were cloned using the TOPO TA Cloning Kit following the standard protocol.
Two to three clones of each individual restriction fragment length polymorphism pattern were sequenced at GATC Biotech. All sequences were deposited with the GenBank database.

Saker, M. L., Jungblut, A. D., Neilan, B. A., Rawn, D. F. K. & Vasconcelos, V. M. Detection of microcystin synthetase genes in health food supplements containing the freshwater cyanobacterium Aphanizomenon flos-aquae. Toxicon 46, 555?562 (2005).

Study extent description: Samples were collected on Byers Peninsula (ASPA 126) in February 2009. This area is a summer snow- and ice-free area. Various microbial mats on wet soil, meltwater and seepages were sampled. 
Sampling description: Cyanobacterial samples were collected from five
different sampling sites in the Antarctic Specially Protected Area
(ASPA) No. 126 of Byers Peninsula, Livingston Island (62° 34' 35" to 62° 4' 35" S and 60° 54' 14" to 61° 13' 07" W), South Shetland Islands, Antarctic Peninsula, during an expedition in February 2009. 
Microbial mats were probed using a sterile spatula, sealed in sterile plastic bags or tubes and stored frozen (-20 °C) for DNA extraction.
Quality control description:  Data were published in a peer-reviewed journal DOI: 10.1038/NCLIMATE1418

Metadata language: English
Date of metadata creation: 2017-06-20
Hierarchy level: Dataset