https://ipt.biodiversity.aq/resource?r=antarctic_desert_surface_soil_microbiome Antarctic polar desert surface soil microbiome Mukan Ji Australian Centre for Astrobiology
Randwick AU
Chris Greening Monash University
Clayton AU
Inka Vanwonterghem University of Queensland
St Lucia AU
Carlo Carere Wairakei Research Centre
Wairakei NZ
Sean Bay Monash University
Clayton AU
Jason Steen University of Queensland
St. Lucia AU
Kate Montgomery Australian Centre for Astrobiology
Randwick AU
Thomas Lines Monash University
Clayton AU
Josie van Dorst Australian Centre for Astrobiology
Randwick AU
Ian Snape Australian Antarctic Division
Kingston AU
Matthew Stott Wairakei Research Centre
Wairakei NZ
Philip Hugenholtz University of Queensland
St. Lucia AU
Belinda Ferrari Australian Centre for Astrobiology
Randwick AU
Maxime Sweetlove Royal Belgian Institute of Natural Sciences Research assistent
Rue Vautier 29 Brussels 1000 BE
msweetlove@naturalsciences.be
user 2019-03-28 eng Whole-genome shotgun sequencing dataset targeting microbes in three soil samples from pristine polar desert at Robinson Ridge (eastern Antarctica) Metadata GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License. Robinson Ridge, east Antarctica 110.586 110.586 -66.37 -66.37 2015-12-13 whole genome shotgun sequencing domain Bacteria Bacteria domain Archaea Archaea domain Eukaryota Eukaryotes domain Viri viruses unkown Mukan Ji Australian Centre for Astrobiology
Randwick AU
Chris Greening Monash University
Clayton AU
Belinda Ferrari Australian Centre for Astrobiology
Randwick AU
Total community DNA was extracted from 0.25–0.3 g of each sample in technical triplicate using the FastDNA SPIN Kit for Soil (MP Biomedicals). All DNA extracts were quantified and DNA lysate quality was evaluated using automated ribosomal intergenic spacer analysis (ARISA). Metagenome libraries were prepared using the Nextera DNA Library Preparation Kit (Illumina) and sequenced using three-fifths of an Illumina HiSeq2000 flowcell lane at the Institute for Molecular Biosciences (University of Queensland). samples were taken at the ice free Robinson Ridge (−66.367739, 110.585262), located 10 km south of Casey station in the Windmill Islands coast of Wilkes Land, is part of a pristine polar desert. Three surface soil samples (100 g) from the top 10 cm of the soil profile were collected in December 2005. Samples were collected along a spatially explicit sampling design comprised of three 300-m-long transects, separated by 2-m distances from each other. Samples were sieved down to 63 μm, aliquoted into 5–25-g subsamples, and stored at −80 °C until analysis. Bioplatforms Australia Mukan Ji This work was supported by Bioplatforms Australia, an Australian Antarctic Science project grant (4406), an ARC Future Fellowship (FT170100341), an ARC DECRA Fellowship (DE170100310), an ARC DORA and Laureate Fellowship (DP120103498 and FL150100038), and a Marsden Grant (16-GNS-035). UNSW Sydney, the Centre for Geometric Biology (Monash University), and the Geothermal Resources of New Zealand research program (GNS Science) also provided funding to support this research.
2019-03-28T10:01:58.053+01:00 dataset Ji M, Greening C, Vanwonterghem I, Carere C, Bay S, Steen J, Montgomery K, Lines T, van Dorst J, Snape I, Stott M, Hugenholtz P, Ferrari B (2019): Antarctic polar desert surface soil microbiome. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=antarctic_desert_surface_soil_microbiome&v=1.0 Ji, M., Greening, C., Vanwonterghem, I., Carere, C. R., Bay, S. K., Steen, J. A., ... & Snape, I. (2017). Atmospheric trace gases support primary production in Antarctic desert surface soil. Nature, 552(7685), 400.