https://ipt.biodiversity.aq/resource?r=methanobasealaska
Bacteria and Archaea biodiversity in Arctic and Subarctic terrestrial ecosystems in Alaska
Léa
Cabrol
Institut méditerranéen d'Océanologie
Researcher
Marseille
FR
Maialen
Barret
ECOLAB, Université de Toulouse
Associate professor
Toulouse
FR
Frederic
Thalasso
CINVESTAV
Professor
Mexico
MX
Laure
Gandois
ECOLAB, Université de Toulouse
Researcher
Toulouse
FR
Céline
Lavergne
Pontificia Universidad Catholica de Valparaiso
Postdoc
Valparaiso
CL
Karla
Martinez Cruz
Universidad de Magallanes
Associate professor
Punta Arenas
CL
Armando
Sepulveda Jaureguy
Universidad de Magallanes
Postdoc
Punta Arenas
CL
Gilberto Javier
Fochesatto
University of Alaska Fairbanks
Associate professor
Fairbanks
US
Claudia
Etchebehere
Biological Research Institute Clemente Estable
Researcher
montevideo
UY
Maialen
Barret
maialen.barret@ensat.fr
Maialen
Barret
maialen.barret@ensat.fr
user
2019-03-19
eng
Methane emissions from aquatic and terrestrial ecosystems play a crucial role in global warming, which is particularly affecting high-latitude ecosystems. As major contributors to methane emissions in natural environments, the microbial communities involved in methane production and oxidation deserve a special attention. Microbial diversity and activity are expected to be strongly affected by the already observed (and further predicted) temperature increase in high-latitude ecosystems, eventually resulting in disrupted feedback methane emissions. The METHANOBASE project has been designed to investigate the intricate relations between microbial diversity and methane emissions in Arctic, Subarctic and Subantarctic ecosystems, under natural (baseline) conditions and in response to simulated temperature increments. We report here a small subunit ribosomal RNA (16S rRNA) analysis of lake, peatland and mineral soil ecosystems.
metadata
GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml
methane
greenhouse gas
bacteria
archaea
procaryote
peatland
wetland
soil
lake
sediment
metabarcoding
16S rRNA
MiSeq
n/a
This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License.
Alaska
-150.8
-147.65
68.62
63.21
2016-06-27
2016-06-27
Bacteria and Archaea
Bacteria
Archaea
unkown
Maialen
Barret
ECOLAB, Université de Toulouse
Associate professor
Toulouse
FR
Léa
Cabrol
Institut méditerranéen d'Océanologie
Researcher
Marseille
FR
After collection, samples were stored at 4°C prior to further processing. Liquid samples were filtered at 0.45µm until clogging and the filters were stored at -20°C. DNA was extracted from these filters using the PowerWater DNA isolation kit (MOBIO) while DNA was extracted from solid samples using the PowerSoil DNA isolation kit (MOBIO). DNA extracts were kept at -20°C. The V4-V5 region of 16S rRNA gene was amplified in the following conditions: 515F and 928R primers (Wang & Qian, 2009. doi:10.1371/journal.pone.0007401), 2min at 94°C, 30 cycles of 60s at 94°C, 40s at 65°C and 30s at 72°C, and 10 min at 72°C. Amplicon sequencing was carried out with Illumina MiSeq technology (2x250pb, V3). Denoising of the sequences dataset and OTU clustering was carried using the FROGS pipeline (Auer et al., 2017. doi:10.1093/bioinformatics/btx791). BLAST was used for taxonomic affiliation.
Samples were collected in summer 2015, without any temporal replication.
A total of 19 ecosystems were studied in Alaska, USA. The selected sites are representative of this Subantarctic region: lakes, peatlands, Nothofagus forest, pampa
In each site, various samples were collected to take into account the local heterogeneity: different depths in water column and sediments, soil horizons, hollows/edges/hummocks.
Water samples were collected with a Van Dorn bottle. Sediments were sampled thanks to a grab-sampler, peat monoliths (approximately 30*30*30cm) were cut with a bread-knife and soil monoliths with a shovel.
Methanobase
Maialen
Barret
METHANOgenic Biodiversity and activity in Arctic, subarctic and Subantarctic Ecosystems affected by climate change
ERANET-LAC joint call 2014
Alaska
Lakes (water, sediments), peatlands (hollows, edges, hummocks) and mineral soils
The METHANOBASE project has been designed to investigate the intricate relations between microbial diversity and methane emissions in Arctic, Subarctic and Subantarctic ecosystems, under natural (baseline) conditions and in response to simulated temperature increments.
2018-04-11T10:10:40.470+02:00
dataset
Cabrol L, Barret M, Thalasso F, Gandois L, Lavergne C, Martinez Cruz K, Sepulveda Jaureguy A, Fochesatto G J, Etchebehere C (2018): Bacteria and Archaea biodiversity in Arctic and Subarctic terrestrial ecosystems in Alaska. v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=methanobasealaska&v=1.2
http://ipt.biodiversity.aq/logo.do?r=methanobasealaska
https://ipt.biodiversity.aq/resource?id=methanobasealaska/v1.2.xml