https://ipt.biodiversity.aq/resource?r=methanobasealaska Bacteria and Archaea biodiversity in Arctic and Subarctic terrestrial ecosystems in Alaska Léa Cabrol Institut méditerranéen d'Océanologie Researcher
Marseille FR
Maialen Barret ECOLAB, Université de Toulouse Associate professor
Toulouse FR
Frederic Thalasso CINVESTAV Professor
Mexico MX
Laure Gandois ECOLAB, Université de Toulouse Researcher
Toulouse FR
Céline Lavergne Pontificia Universidad Catholica de Valparaiso Postdoc
Valparaiso CL
Karla Martinez Cruz Universidad de Magallanes Associate professor
Punta Arenas CL
Armando Sepulveda Jaureguy Universidad de Magallanes Postdoc
Punta Arenas CL
Gilberto Javier Fochesatto University of Alaska Fairbanks Associate professor
Fairbanks US
Claudia Etchebehere Biological Research Institute Clemente Estable Researcher
montevideo UY
Maialen Barret maialen.barret@ensat.fr Maialen Barret maialen.barret@ensat.fr user 2019-03-19 eng Methane emissions from aquatic and terrestrial ecosystems play a crucial role in global warming, which is particularly affecting high-latitude ecosystems. As major contributors to methane emissions in natural environments, the microbial communities involved in methane production and oxidation deserve a special attention. Microbial diversity and activity are expected to be strongly affected by the already observed (and further predicted) temperature increase in high-latitude ecosystems, eventually resulting in disrupted feedback methane emissions. The METHANOBASE project has been designed to investigate the intricate relations between microbial diversity and methane emissions in Arctic, Subarctic and Subantarctic ecosystems, under natural (baseline) conditions and in response to simulated temperature increments. We report here a small subunit ribosomal RNA (16S rRNA) analysis of lake, peatland and mineral soil ecosystems. metadata GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml methane greenhouse gas bacteria archaea procaryote peatland wetland soil lake sediment metabarcoding 16S rRNA MiSeq n/a This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License. Alaska -150.8 -147.65 68.62 63.21 2016-06-27 2016-06-27 Bacteria and Archaea Bacteria Archaea unkown Maialen Barret ECOLAB, Université de Toulouse Associate professor
Toulouse FR
Léa Cabrol Institut méditerranéen d'Océanologie Researcher
Marseille FR
After collection, samples were stored at 4°C prior to further processing. Liquid samples were filtered at 0.45µm until clogging and the filters were stored at -20°C. DNA was extracted from these filters using the PowerWater DNA isolation kit (MOBIO) while DNA was extracted from solid samples using the PowerSoil DNA isolation kit (MOBIO). DNA extracts were kept at -20°C. The V4-V5 region of 16S rRNA gene was amplified in the following conditions: 515F and 928R primers (Wang & Qian, 2009. doi:10.1371/journal.pone.0007401), 2min at 94°C, 30 cycles of 60s at 94°C, 40s at 65°C and 30s at 72°C, and 10 min at 72°C. Amplicon sequencing was carried out with Illumina MiSeq technology (2x250pb, V3). Denoising of the sequences dataset and OTU clustering was carried using the FROGS pipeline (Auer et al., 2017. doi:10.1093/bioinformatics/btx791). BLAST was used for taxonomic affiliation. Samples were collected in summer 2015, without any temporal replication. A total of 19 ecosystems were studied in Alaska, USA. The selected sites are representative of this Subantarctic region: lakes, peatlands, Nothofagus forest, pampa In each site, various samples were collected to take into account the local heterogeneity: different depths in water column and sediments, soil horizons, hollows/edges/hummocks. Water samples were collected with a Van Dorn bottle. Sediments were sampled thanks to a grab-sampler, peat monoliths (approximately 30*30*30cm) were cut with a bread-knife and soil monoliths with a shovel. Methanobase Maialen Barret METHANOgenic Biodiversity and activity in Arctic, subarctic and Subantarctic Ecosystems affected by climate change ERANET-LAC joint call 2014 Alaska Lakes (water, sediments), peatlands (hollows, edges, hummocks) and mineral soils The METHANOBASE project has been designed to investigate the intricate relations between microbial diversity and methane emissions in Arctic, Subarctic and Subantarctic ecosystems, under natural (baseline) conditions and in response to simulated temperature increments.
2018-04-11T10:10:40.470+02:00 dataset Cabrol L, Barret M, Thalasso F, Gandois L, Lavergne C, Martinez Cruz K, Sepulveda Jaureguy A, Fochesatto G J, Etchebehere C (2018): Bacteria and Archaea biodiversity in Arctic and Subarctic terrestrial ecosystems in Alaska. v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=methanobasealaska&v=1.2 http://ipt.biodiversity.aq/logo.do?r=methanobasealaska https://ipt.biodiversity.aq/resource?id=methanobasealaska/v1.2.xml