https://ipt.biodiversity.aq/resource?r=tango-stable-isotope tango-stable-isotope Manon Bayat Université Libre de Bruxelles

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manon.bayat@gmail.com 0000-0002-0985-4243 Ginevra Lilli Université de Montpellier

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ginevra.lilli@umontpellier.fr 0009-0006-7089-9287 Isabelle F. George Université Libre de Bruxelles

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isabelle.george@ulb.be 0000-0003-1616-5232 Lucas Terrana Université Libre de Bruxelles

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0000-0001-5767-3220 Camille Moreau Université Libre de Bruxelles

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Camille.Moreau@ulb.be 0000-0002-0981-7442 Bruno Danis Université Libre de Bruxelles

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Bruno.Danis@ulb.be 0000-0002-9037-7623 Martin Dogniez Université de Liège

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martin.dogniez@uliege.be 0000-0002-9708-1761 Loïc N. Michel Université de Liège

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loic.michel@uliege.be 0000-0003-0988-7050 Manon Bayat Université Libre de Bruxelles

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manon.bayat@gmail.com 0000-0002-0985-4243 Loïc N. Michel Université de Liège

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loic.michel@uliege.be 0000-0003-0988-7050 Yi Ming Gan Royal Belgian institute of Natural Sciences

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ymgan@naturalsciences.be 0000-0001-7087-2646 publisher Loïc N. Michel Université de Liège

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0000-0003-0988-7050 2026-02-13 eng "Stable isotope ratios and elemental contents of C, N and S in an Antarctic sea urchin, Sterechinus neumayeri, sampled during the TANGO1 and TANGO2 expeditions in the Western Antarctic Peninsula (2023, 2024)” is a sampling event type dataset published by SCAR-AntOBIS. This dataset supported the elaboration of the paper entitled “Realized dietary specialization in an Antarctic keystone species, the sea urchin Sterechinus neumayeri” by BAYAT M., LILLI G., GEORGE I. F., TERRANA L., MOREAU C., DANIS B., DOGNIEZ M. and MICHEL L. N., submitted in the Marine Biology journal. The reports (in English) are available at: https://doi.org/10.5281/zenodo.8013722 https://doi.org/10.5281/zenodo.11653689 This dataset contains records of 250 specimens of Sterechinus neumayeri based on SCUBA diving collection during the TANGO1 and TANGO2 expeditions along the Western Antarctic Peninsula, from 13 February to 19 March 2023 and from 5 February to 8 March 2024, respectively. The data is published as a standardized Darwin Core Archive and includes for each sampling event an eventID, date, time, location, depth range when applicable, and sampling protocol (in the event core). For each occurrence, an occurrenceID, materialEntityID, organismID, siteID, eventID, event information, sampling protocol, depth information, latitude and longitude, status (present/absent), taxa classification, samples identifiers, preparations, specimens measurements, stable isotope ratios and expeditions whenever applicable are specified in the occurrence extension. Each measurement associated with a sampling event or an occurrence record has a measurementID, measurement type, measurement value and measurement unit in the measurement extension. This dataset is part of the TANGO project funded by the Belgian Science Policy Office through the BRAIN-be 2.0 programme (BELSPO project B2/212/P1/TANGO). This dataset is published as open data with technical support provided by the SCAR Antarctic Biodiversity Portal (biodiversity.aq) (BELSPO project RT/23/ADVANCE). Please follow the guidelines from the SCAR Data Policy (SCAR, 2023) when using the data. For any inquiry about this dataset, please contact us via the contact information provided in the metadata or by email at data-biodiversity-aq@naturalsciences.be. Issues with this dataset can be reported at: https://github.com/biodiversity-aq/data-publication/ OCCURRENCE GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml ECHINODERMS; SEA URCHINS; TROPHIC DYNAMICS; MARINE ECOSYSTEMS; BENTHIC; COASTAL; STABLE ISOTOPES; SOUTHERN OCEAN; POLAR; SOUTHERN HEMISPHERE; Specimen GCMD Science Keywords: https://gcmd.earthdata.nasa.gov/KeywordViewer/ Specimen GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml This dataset led to the writing of the following paper: “Realized dietary specialization in an Antarctic keystone species, the sea urchin Sterechinus neumayeri” by BAYAT M., LILLI G., GEORGE I. F., TERRANA L., MOREAU C., DANIS B., DOGNIEZ M. and MICHEL L. N. (2026) Marine Biology journal. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License. Creative Commons Attribution 4.0 International https://spdx.org/licenses/CC-BY-4.0.html CC-BY-4.0 West Antarctic Peninsula -67.2475 -60.6864 -62.9267 -67.5519 2023-02-20 2024-02-28 N/A class Echinoidea order Camarodonta family Echinidae kingdom Animalia phylum Echinodermata This research was funded by the Belgian Science Policy Office (BELSPO) through the BRAIN-be 2.0 project TANGO (https://www.belspo.be/belspo/brain2-be/projects/TANGO_en.pdf, contract nr. B2/212/P1/TANGO). This dataset is published as open data with technical support provided by SCAR Antarctic Biodiversity Portal (biodiversity.aq) (BELSPO project RT/23/ADVANCE). asNeeded Manon Bayat Université Libre de Bruxelles

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manon.bayat@gmail.com 0000-0002-0985-4243 Loïc N. Michel Université de Liège

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loic.michel@uliege.be 0000-0003-0988-7050 1. Sample preservation: Samples of Sterechinus neumayeri were stored individually in glass vials and grouped in large bags per station 2. Sample preparation: Once dissected onboard to only keep the Aristotle’s lantern with the associated muscles, samples were primarily dried at 60°C then kept frozen at -20°C, and shipped to the Freshwater and Ocean Science Unit of research at the University of Liege, Belgium. At the lab, samples were dried at 60°C or 72 hours, and homogenized into a powder prior to SIA using a mortar and pestle. 3. Stable isotope analysis: For each individual, approximately 2.5 mg of homogenized muscle tissue were weighed into tin capsules. Isotopic ratios were expressed using the widespread δ notation (Coplen, 2011), in ‰ and relative to the international references Vienna Pee Dee Belemnite (for carbon), atmospheric air (for nitrogen) and Vienna Canyon Diablo Troilite (for sulphur). IAEA (International Atomic Energy Agency, Vienna, Austria) certified reference materials caffeine (IAEA-600; δ13C = −27.771 ± 0.043‰ ; δ15N = 1.0 ± 0.2‰; means ± SD), and barium sulphate (IAEA-SO-5; δ34S = 0.5 ± 0.2‰ ) were used as primary analytical standards. Sulfanilic acid (Sigma-Aldrich; δ13C = −25.6 ± 0.4‰; δ15N = −0.13 ± 0.4‰; δ34S = 5.9 ± 0.5‰; means ± SD) was used as secondary analytical standard. Standard deviations on multi-batch replicate measurements of secondary and internal lab standards (amphipod crustacean muscle) analysed interspersed with samples (one replicate of each standard every 15 analyses) were 0.2‰ for both δ13C and δ15N and 0.4‰ for δ34S. 4. Instrumentation: These ratios were measured by continuous-flow elemental analysis–isotope ratio mass spectrometry (CF-EA-IRMS) at the University of Liège, using a vario MICRO Cube elemental analyser (Elementar Analysensysteme GmbH, Hanau, Germany) coupled to a precisION mass spectrometer (Elementar UK, Cheadle, UK). Study extent: This dataset describes the stable isotope ratios and element contents of C, N and S in sea urchin Sterechinus neumayeri specimens sampled during TANGO1 and TANGO2 expeditions in the Western Antarctic Peninsula (from February to March 2023, 2024). Please check the paper for more detailed information on sampling and sample preparation. All samples were taken thirteen different stations along the Western Antarctic Peninsula. Most samples were hand collected by SCUBA divers between 5 and 20 meters depth. They consisted only in Sterechinus neumayeri specimens. - All records were validated. - Coordinates were converted into decimal latitude and decimal longitude and plotted on map to verify the actual geographical location corresponds to its locality. - All scientific names were checked for typo and matched to the species information backbone of Worlds Register of Marine Species (http://marinespecies.org/) and LSID were assigned to each taxa as scientificNameID. - Event date and time were verified to be compliant with ISO 8601 standard Manon Bayat 0000-0002-0985-4243 author Ginevra Lilli 0009-0006-7089-9287 author Isabelle F. George 0000-0003-1616-5232 author Lucas Terrana 0000-0001-5767-3220 author Camille Moreau 0000-0002-0981-7442 author Bruno Danis 0000-0002-9037-7623 author Martin Dogniez 0000-0002-9708-1761 author Loïc N. Michel 0000-0003-0988-7050 principalInvestigator The TANGO1&2 expedition ventured to accumulate new data on the responses of marine ecosystems to shifts in ice regimes in the West Antarctic Peninsula (WAP), taking full advantage of a nimble sampling platform, the R/V Australis, a steel hulled, fully rigged motor sailer. TANGO1 took place between February and March 2023 and 2024, sampling two main locations at different spatial scales. Deploying 14 different types of gear (both traditional and modern), The use of R/V Australis for coastal studies deemed to be extremely efficient, in terms of environmental impact and reactivity, allowing the team to adapt the sampling efforts in function of the weather or anchoring conditions. Fully devoted to the expedition, the ship allowed the Tango team to sample in shallow areas, not accessible to icebreakers and too far away from research stations, and which have been under sampled. This work was supported by the “Estimating Tipping points in habitability of ANtarctic benthic ecosystems under GlObal future climate change scenarios” project (TANGO; B2/212/P1/TANGO) funded by the ‘Belgian Science Policy Office (BELSPO) Yi-Ming Gan 0000-0001-7087-2646 2026-02-06T20:26:02.243+01:00 dataset Bayat M, Lilli G, George I F, Terrana L, Moreau C, Danis B, Dogniez M, Michel L N (2026). Stable isotope ratios and elemental contents of C, N and S in an Antarctic sea urchin, Sterechinus neumayeri, sampled during the TANGO1 and TANGO2 expeditions in the Western Antarctic Peninsula (2023, 2024). Version 1.0. SCAR - AntOBIS. Occurrence dataset. https://ipt.biodiversity.aq/resource?r=tango-stable-isotope&v=1.0 1. The Scientific Committee on Antarctic Research. (2023). SCAR Report 42 - September 2022 - SCAR Data Policy (2022). Zenodo. Danis, B., Amenábar, C. M., Bombosch, A., Brusselman, A., Buydens, M., Delille, B., Dogniez, M., Katz, L., Moreau, C., Pasotti, F., Robert, H., Wallis, B. (2023) Report of the TANGO1 expedition to the West Antarctic Peninsula. Coplen, T.B. (2011), Guidelines and recommended terms for expression of stable-isotope-ratio and gas-ratio measurement results. Rapid Commun. Mass Spectrom., 25: 2538-2560. Danis, B., Bayat, M., Brusselman, A., Coerper, A., De Borger, E., Delille, B., Dogniez, M., Katz, L., Moreau, C., Reade, A., Robert, H., Terrana, L., Voisin, A., Wallis, B. (2024) Report of the TANGO2 expedition to the West Antarctic Peninsula.