7ebcc399-ad1f-4263-86a9-f612b1454a4a https://ipt.biodiversity.aq/resource?r=western_peninsula_microbes Western Antarctic Peninsula Bacteria (Mesocosm exp.) Catherine Luria Brown University
US
Linda Amaral-Zettler Royal Netherlands Institute for Sea Research
NL
Hugh Ducklow Lamont-Doherty Earth Observatory
US
Daniel Repeta Woods Hole Oceanographic Institution
Woods Hole US
Andrew Rhyne Roger Williams University
Bristol US
Jeremy Rich University of Maine
Walpole US
Maxime Sweetlove Royal Belgian Institude of Natural Sciences Assistent researcher
Rue Vautier 29 Brussels 1000 BE
msweetlove@naturalsciences.be
2019-03-19 eng Amplicon sequencing data of the 16S SSU rRNA marker gene of bacteria in four Mesososm experiments that spanned the spring transitional period (August–December 2013) in surface waters of the Western Antarctic Peninsula (WAP). Each mesocosm consisted of nearshore surface seawater (50 L) incubated in the laboratory for 10 days. Further details in: Luria, C. M., Amaral-Zettler, L. A., Ducklow, H. W., Repeta, D. J., Rhyne, A. L., & Rich, J. J. (2017). Seasonal shifts in bacterial community responses to phytoplankton-derived dissolved organic matter in the Western Antarctic Peninsula. Frontiers in microbiology, 8, 2117. Metadata GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License. Sea off the Western Antarctic Peninsula -64.18 -64.05 -64.77 -64.77 Bacteria (16S ssu rRNA gene, v6 region) domain Bacteria Bacteria unkown Linda Amaral-Zettler Royal Netherlands Institute for Sea Research
NL
Jeremy Rich University of Maine
193 Clarks Cove Road Walpole 04573 US
Mesocosms were set up at Palmer Station, Antarctica, using seawater from the station’s intake, located at a depth of 6 m, 16 m from the shore of the station. This source water was monitored during the experimental period (August–December 2013) to determine any changes in environmental parameters that might influence bacterial community responses in mesocosm experiments. Samples for dissolved nutrients (phosphate, silicate, nitrite, and nitrate) and particulate organic carbon and nitrogen (POC and PN) were processed according to Palmer LTER standard protocols Western Antarctic Peninsula Mesocosms were set up at Palmer Station, Antarctica, using seawater from the station’s intake, located at a depth of 6 m, 16 m from the shore of the station. This source water was monitored during the experimental period (August–December 2013) to determine any changes in environmental parameters that might influence bacterial community responses in mesocosm experiments. Samples for dissolved nutrients (phosphate, silicate, nitrite, and nitrate) and particulate organic carbon and nitrogen (POC and PN) were processed according to Palmer LTER standard protocols Western Antarctic Peninsula Bacteria (Mesocosm exp.) Catherine Lucia Linda Amaral-Zettler Hugh Ducklow Daniel Repeta Andrew Rhyne Jeremy Rich CL was partially funded by the Graduate School and the Department of Ecology and Evolutionary Biology at Brown University and the Brown University-Marine Biological Laboratory Joint Graduate Program. This material is based upon work supported by the National Science Foundation under Grant Nos. ANT-1142114 to LA-Z, OPP-0823101 and PLR-1440435 to HD, and ANT-1141993 to JR. The Gordon and Betty Moore Foundation grant 1711 supported work by DR. Western Antarctic Peninsula Mesocosm experiments were conducted in August, September, October, and December 2013 at Palmer Station by filling acid-washed 50 L polycarbonate carboys with seawater from the station’s intake. The filled carboys were divided into two treatments: (1) control (no additions, n = 3) and (2) +DOM (carboys that received diatom exudates; n = 3). An additional f/2 control treatment (uninoculated growth media, n = 3) was included in the October experiment only. The DOC concentration in the f/2 control was not any different than controls without any added DOC. As such, the f/2 control served as an additional no DOC addition control. The mesocosms were incubated for 10 days at 0°C, except in the August experiment when mesocosms were incubated at 3°C. All experiments were conducted under a low-level of continuous light, 1 × 1014 quanta cm-2 s-1, as measured inside the carboys with a hand-held radiometer (Biospherical Instruments, Inc.).
2018-11-08T10:33:31.321+01:00 dataset Luria C, Amaral-Zettler L, Ducklow H, Repeta D, Rhyne A, Rich J (2018): Western Antarctic Peninsula Bacteria (Mesocosm exp.). v1.4. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=western_peninsula_microbes&v=1.4 Luria, C. M., Amaral-Zettler, L. A., Ducklow, H. W., Repeta, D. J., Rhyne, A. L., & Rich, J. J. (2017). Seasonal shifts in bacterial community responses to phytoplankton-derived dissolved organic matter in the Western Antarctic Peninsula. Frontiers in microbiology, 8, 2117. 2009-2014 7ebcc399-ad1f-4263-86a9-f612b1454a4a/v1.4.xml