Bacteria on mummified seals in the Antarctic Dry Valleys

Versão mais recente publicado por SCAR - Microbial Antarctic Resource System em Mar 19, 2019 SCAR - Microbial Antarctic Resource System

Amplicon dataset of Bacteria (16S ssu rRNA) living around mummified seal carcasses in the Antarctic Dry Valleys

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Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Tiao G, Lee C, McDonald I, Cowan D, Cary C (2019): Bacteria on mummified seals in the Antarctic Dry Valleys. v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=antarctic_dry_valley_mummified_seal_microbiome&v=1.2

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O editor e o detentor dos direitos deste trabalho é SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: a3fb3af6-724d-4de3-ace8-4fa21cc729ed.  SCAR - Microbial Antarctic Resource System publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por Scientific Committee on Antarctic Research.

Palavras-chave

Metadata

Contatos

Quem criou esse recurso:

Grace Tiao
University of Waikato Hamilton NZ
Charles Lee
University of Waikato Hamilton NZ
Ian McDonald
University of Waikato Hamilton NZ
Donald Cowan
University of the Western Cape Cape Town ZA
Craig Cary
University of Waikato Hamilton NZ

Quem pode responder a perguntas sobre o recurso:

Grace Tiao
University of Waikato Hamilton NZ

Quem preencher os metadados:

Maxime Sweetlove
Research assistent
Royal Belgian Institute for Natural Sciences Rue Vautier 29 1000 Brussels

Quem mais foi associado com o recurso:

Usuário

Cobertura Geográfica

soil samples from under and around mummified crabeater seal near Lake Miers (S 78°05′36″, E 163°51′22″), Miers Valley, Victoria Land, Antarctica, that was transported to a similar site 20 m north.

Coordenadas delimitadoras Sul Oeste [-78.933, 163.856], Norte Leste [-78.933, 163.856]

Cobertura Taxonômica

Amplicin sequencing of Bacteria (16S ssu rRNA gene)

Domínio  Bacteria (Bacteria)

Cobertura Temporal

Data Inicial / Data final 2005-01-15 / 2009-01-15

Dados Sobre o Projeto

Nenhuma descrição disponível

Título Seal Movement
Financiamento This work was supported through grants from the New Zealand Foundation for Research Science and Technology, Antarctica New Zealand, and the National Science Foundation (OPP-0739648 and 0944560). Additional support was provided by a George Peabody Gardner Fellowship, by the New Zealand Foundation for Research, Science and Technology Postdoctoral Research Fellowship and the New Zealand Marsden Foundation (UOW1003).

O pessoal envolvido no projeto:

Grace Tiao

Métodos de Amostragem

Soil samples (>10 g each) were collected aseptically at a depth of 0.05m. Samples were stored in sterile plastic containers at −80 °C until analysis.

Área de Estudo On 30 January 2006, a mummified crabeater seal was carried from its original resting site near Lake Miers (S 78°05′36″, E 163°51′22″), Miers Valley, Victoria Land, Antarctica, to a new site of similar geomorphology 20 m north. Samples for amplicon sequencing were taken from the original site before transportation (austral summer 2005), the new site before transportation (control, austral summer 2006), and the new site after incubation (austral summer 2009)

Descrição dos passos do método:

  1. 16S rRNA gene were generated using the primer pair Tx9 (5′-GGATTAGAWACCCBGGTAGTC-3′) and 1391R (5′-GACGGGCRGTGWGTRCA-3′). PCR was performed in triplicate and pooled to reduce stochastic variability between reactions. Each 30 μl reaction contained 5 or 20 ng of extracted community DNA, Pfx polymerase and platinum polymerase (0.5 U each; Invitrogen), 1× Pfx PCR buffer with Pfx enhancer, 0.2 mM dNTPs, 1 mM MgCl2, 0.02 mg ml−1 BSA, 0.8 μM of forward and reverse primer, and PCR-grade water. Thermal cycling conditions were 94 °C for 2 min; 18 or 24 cycles of 94 °C for 15 s, 55 °C for 30 s and 68 °C for 1 min; and 68 °C for 3 min.
  2. Amplicons were size-selected and purified using polyacrylamide gel electrophoresis before being prepared for pyrosequencing by Taxon Biosciences (Tiburon, CA, USA).

Citações bibliográficas

  1. Tiao, G., Lee, C. K., McDonald, I. R., Cowan, D. A., & Cary, S. C. (2012). Rapid microbial response to the presence of an ancient relic in the Antarctic Dry Valleys. Nature Communications, 3, 660.

Metadados Adicionais

Identificadores alternativos a3fb3af6-724d-4de3-ace8-4fa21cc729ed
https://ipt.biodiversity.aq/resource?r=antarctic_dry_valley_mummified_seal_microbiome