cyanobacterial mats of from the Byers Peninsula, Antarctica

Última versión Publicado por SCAR - Microbial Antarctic Resource System en Mar 19, 2019 SCAR - Microbial Antarctic Resource System

Cyanobacterial 16S rRNA gene sequences from cyanobacterial mats of Antarctic (Byers Peninsula) origin obtained by clone library

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El publicador y propietario de los derechos de este trabajo es SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

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Metadata

Contactos

¿Quién creó el recurso?:

Julia Kleinteich
Researcher
University of Liège Liège Lieège BE

¿Quién puede resolver dudas acerca del recurso?:

Julia Kleinteich
Researcher
University of Liège Liège Lieège BE
Daniel R Dietrich
Researcher
University of Konstanz Konztanz DE

¿Quién documentó los metadatos?:

Julien Cigar

Cobertura Geográfica

Byers Peninsula, Livingston Island, Antarctica

Coordenadas límite Latitud Mínima Longitud Mínima [-62.7, -61.5], Latitud Máxima Longitud Máxima [-62.5, -61]

Cobertura Taxonómica

All data provided are OTUs of the 16S rRNA gene which were identified via a comparison to online databases to family or genus level. For most OTUs identification to species level was not possible

Filo  Cyanobacteria (Cyanobacteria)

Cobertura Temporal

Fecha Inicial / Fecha Final 2009-02-01 / 2009-02-28

Datos del Proyecto

No hay descripción disponible

Título DI698/18-1 Dietrich
Fuentes de Financiación Deutsche Forschungsgesellschaft DFG

Personas asociadas al proyecto:

Investigador Principal
Daniel R Dietrich

Métodos de Muestreo

Cyanobacterial samples were collected from five different sampling sites in the Antarctic Specially Protected Area (ASPA) No. 126 of Byers Peninsula, Livingston Island (62° 34' 35" to 62° 4' 35" S and 60° 54' 14" to 61° 13' 07" W), South Shetland Islands, Antarctic Peninsula, during an expedition in February 2009. Microbial mats were probed using a sterile spatula, sealed in sterile plastic bags or tubes and stored frozen (-20 °C) for DNA extraction.

Área de Estudio Samples were collected on Byers Peninsula (ASPA 126) in February 2009. This area is a summer snow- and ice-free area. Various microbial mats on wet soil, meltwater and seepages were sampled.
Control de Calidad Data were published in a peer-reviewed journal DOI: 10.1038/NCLIMATE1418

Descripción de la metodología paso a paso:

  1. After collection the samples were stored at -20°C until further processing. DNA was extracted from the samples in three replicates and combined. 16S rRNA genes were amplified using cyanobacteria specific primers (Saker et al. 2005). PCR Products were cloned using the TOPO TA Cloning Kit following the standard protocol. Two to three clones of each individual restriction fragment length polymorphism pattern were sequenced at GATC Biotech. All sequences were deposited with the GenBank database. Saker, M. L., Jungblut, A. D., Neilan, B. A., Rawn, D. F. K. & Vasconcelos, V. M. Detection of microcystin synthetase genes in health food supplements containing the freshwater cyanobacterium Aphanizomenon flos-aquae. Toxicon 46, 555?562 (2005).

Metadatos Adicionales

Identificadores Alternativos https://ipt.biodiversity.aq/resource?r=cyano_16s