cyanobacterial mats of from the Byers Peninsula, Antarctica

最新バージョン SCAR - Microbial Antarctic Resource System によって公開 Mar 19, 2019 SCAR - Microbial Antarctic Resource System

Cyanobacterial 16S rRNA gene sequences from cyanobacterial mats of Antarctic (Byers Peninsula) origin obtained by clone library

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権利

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パブリッシャーとライセンス保持者権利者は SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

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キーワード

Metadata

連絡先

リソースを作成した人:

Julia Kleinteich
Researcher
University of Liège Liège Lieège BE

リソースに関する質問に答えることができる人:

Julia Kleinteich
Researcher
University of Liège Liège Lieège BE
Daniel R Dietrich
Researcher
University of Konstanz Konztanz DE

メタデータを記載した人:

Julien Cigar

地理的範囲

Byers Peninsula, Livingston Island, Antarctica

座標(緯度経度) 南 西 [-62.7, -61.5], 北 東 [-62.5, -61]

生物分類学的範囲

All data provided are OTUs of the 16S rRNA gene which were identified via a comparison to online databases to family or genus level. For most OTUs identification to species level was not possible

Phylum  Cyanobacteria (Cyanobacteria)

時間的範囲

開始日 / 終了日 2009-02-01 / 2009-02-28

プロジェクトデータ

説明がありません

タイトル DI698/18-1 Dietrich
ファンデイング Deutsche Forschungsgesellschaft DFG

プロジェクトに携わる要員:

研究代表者
Daniel R Dietrich

収集方法

Cyanobacterial samples were collected from five different sampling sites in the Antarctic Specially Protected Area (ASPA) No. 126 of Byers Peninsula, Livingston Island (62° 34' 35" to 62° 4' 35" S and 60° 54' 14" to 61° 13' 07" W), South Shetland Islands, Antarctic Peninsula, during an expedition in February 2009. Microbial mats were probed using a sterile spatula, sealed in sterile plastic bags or tubes and stored frozen (-20 °C) for DNA extraction.

Study Extent Samples were collected on Byers Peninsula (ASPA 126) in February 2009. This area is a summer snow- and ice-free area. Various microbial mats on wet soil, meltwater and seepages were sampled.
Quality Control Data were published in a peer-reviewed journal DOI: 10.1038/NCLIMATE1418

Method step description:

  1. After collection the samples were stored at -20°C until further processing. DNA was extracted from the samples in three replicates and combined. 16S rRNA genes were amplified using cyanobacteria specific primers (Saker et al. 2005). PCR Products were cloned using the TOPO TA Cloning Kit following the standard protocol. Two to three clones of each individual restriction fragment length polymorphism pattern were sequenced at GATC Biotech. All sequences were deposited with the GenBank database. Saker, M. L., Jungblut, A. D., Neilan, B. A., Rawn, D. F. K. & Vasconcelos, V. M. Detection of microcystin synthetase genes in health food supplements containing the freshwater cyanobacterium Aphanizomenon flos-aquae. Toxicon 46, 555?562 (2005).

追加のメタデータ

代替識別子 https://ipt.biodiversity.aq/resource?r=cyano_16s