cyanobacterial mats of from the Byers Peninsula, Antarctica

Последняя версия опубликована SCAR - Microbial Antarctic Resource System Mar 19, 2019 SCAR - Microbial Antarctic Resource System

Cyanobacterial 16S rRNA gene sequences from cyanobacterial mats of Antarctic (Byers Peninsula) origin obtained by clone library

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Публикующей организацией и владельцем прав на данную работу является SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

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Metadata

Контакты

Кто является создателем ресурса:

Julia Kleinteich
Researcher
University of Liège Liège Lieège BE

Кто может ответить на вопросы о ресурсе:

Julia Kleinteich
Researcher
University of Liège Liège Lieège BE
Daniel R Dietrich
Researcher
University of Konstanz Konztanz DE

Кем заполнены метаданные:

Julien Cigar

Географический охват

Byers Peninsula, Livingston Island, Antarctica

Ограничивающие координаты Юг Запад [-62.7, -61.5], Север Восток [-62.5, -61]

Таксономический охват

All data provided are OTUs of the 16S rRNA gene which were identified via a comparison to online databases to family or genus level. For most OTUs identification to species level was not possible

Phylum  Cyanobacteria (Cyanobacteria)

Временной охват

Дата начала / Дата окончания 2009-02-01 / 2009-02-28

Данные проекта

Описание отсутсвует

Название DI698/18-1 Dietrich
Финансирование Deutsche Forschungsgesellschaft DFG

Исполнители проекта:

Principal Investigator
Daniel R Dietrich

Методы сбора

Cyanobacterial samples were collected from five different sampling sites in the Antarctic Specially Protected Area (ASPA) No. 126 of Byers Peninsula, Livingston Island (62° 34' 35" to 62° 4' 35" S and 60° 54' 14" to 61° 13' 07" W), South Shetland Islands, Antarctic Peninsula, during an expedition in February 2009. Microbial mats were probed using a sterile spatula, sealed in sterile plastic bags or tubes and stored frozen (-20 °C) for DNA extraction.

Охват исследования Samples were collected on Byers Peninsula (ASPA 126) in February 2009. This area is a summer snow- and ice-free area. Various microbial mats on wet soil, meltwater and seepages were sampled.
Контроль качества Data were published in a peer-reviewed journal DOI: 10.1038/NCLIMATE1418

Описание этапа методики:

  1. After collection the samples were stored at -20°C until further processing. DNA was extracted from the samples in three replicates and combined. 16S rRNA genes were amplified using cyanobacteria specific primers (Saker et al. 2005). PCR Products were cloned using the TOPO TA Cloning Kit following the standard protocol. Two to three clones of each individual restriction fragment length polymorphism pattern were sequenced at GATC Biotech. All sequences were deposited with the GenBank database. Saker, M. L., Jungblut, A. D., Neilan, B. A., Rawn, D. F. K. & Vasconcelos, V. M. Detection of microcystin synthetase genes in health food supplements containing the freshwater cyanobacterium Aphanizomenon flos-aquae. Toxicon 46, 555?562 (2005).

Дополнительные метаданные

Альтернативные идентификаторы https://ipt.biodiversity.aq/resource?r=cyano_16s