cyanobacterial mats of from the Byers Peninsula, Antarctica

最新版本 由 SCAR - Microbial Antarctic Resource System 發佈於 Mar 19, 2019 SCAR - Microbial Antarctic Resource System

Cyanobacterial 16S rRNA gene sequences from cyanobacterial mats of Antarctic (Byers Peninsula) origin obtained by clone library

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The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

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聯絡資訊

資源建立者:

Julia Kleinteich
Researcher
University of Liège Liège Lieège BE

可回覆此資源相關問題者:

Julia Kleinteich
Researcher
University of Liège Liège Lieège BE
Daniel R Dietrich
Researcher
University of Konstanz Konztanz DE

元數據填寫者:

Julien Cigar

地理涵蓋範圍

Byers Peninsula, Livingston Island, Antarctica

界定座標範圍 緯度南界 經度西界 [-62.7, -61.5], 緯度北界 經度東界 [-62.5, -61]

分類群涵蓋範圍

All data provided are OTUs of the 16S rRNA gene which were identified via a comparison to online databases to family or genus level. For most OTUs identification to species level was not possible

Phylum  Cyanobacteria (Cyanobacteria)

時間涵蓋範圍

起始日期 / 結束日期 2009-02-01 / 2009-02-28

計畫資料

無相關描述

計畫名稱 DI698/18-1 Dietrich
經費來源 Deutsche Forschungsgesellschaft DFG

The personnel involved in the project:

研究主持人
Daniel R Dietrich

取樣方法

Cyanobacterial samples were collected from five different sampling sites in the Antarctic Specially Protected Area (ASPA) No. 126 of Byers Peninsula, Livingston Island (62° 34' 35" to 62° 4' 35" S and 60° 54' 14" to 61° 13' 07" W), South Shetland Islands, Antarctic Peninsula, during an expedition in February 2009. Microbial mats were probed using a sterile spatula, sealed in sterile plastic bags or tubes and stored frozen (-20 °C) for DNA extraction.

研究範圍 Samples were collected on Byers Peninsula (ASPA 126) in February 2009. This area is a summer snow- and ice-free area. Various microbial mats on wet soil, meltwater and seepages were sampled.
品質控管 Data were published in a peer-reviewed journal DOI: 10.1038/NCLIMATE1418

方法步驟描述:

  1. After collection the samples were stored at -20°C until further processing. DNA was extracted from the samples in three replicates and combined. 16S rRNA genes were amplified using cyanobacteria specific primers (Saker et al. 2005). PCR Products were cloned using the TOPO TA Cloning Kit following the standard protocol. Two to three clones of each individual restriction fragment length polymorphism pattern were sequenced at GATC Biotech. All sequences were deposited with the GenBank database. Saker, M. L., Jungblut, A. D., Neilan, B. A., Rawn, D. F. K. & Vasconcelos, V. M. Detection of microcystin synthetase genes in health food supplements containing the freshwater cyanobacterium Aphanizomenon flos-aquae. Toxicon 46, 555?562 (2005).

額外的元數據

替代的識別碼 https://ipt.biodiversity.aq/resource?r=cyano_16s