Global biogeography of desert cyanobacteria

最新バージョン SCAR - Microbial Antarctic Resource System によって公開 Mar 19, 2019 SCAR - Microbial Antarctic Resource System

Amplicon sequencing dataset (454 pyrosequencing) of Bacteria (16S ssu rRNA) and Cyanobacteria (nifH) in cold desert quartz rocks

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バージョン

次の表は、公にアクセス可能な公開バージョンのリソースのみ表示しています。

引用方法

研究者はこの研究内容を以下のように引用する必要があります。:

Lacap-bugler D, Lee K, Archer S, Gillman L, Lau M, Leuzinger S, Lee C, Maki T, McKay C, Perrott J, de los Rios-Murillo A, Warren-Rhodes K, Hopkins D, Pointing S (2018): Global biogeography of desert cyanobacteria. v1.4. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=desert_cyanobacteria&v=1.4

権利

研究者は権利に関する下記ステートメントを尊重する必要があります。:

パブリッシャーとライセンス保持者権利者は SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF登録

このリソースをはGBIF と登録されており GBIF UUID: 914f39c7-7bb2-4e77-a4ae-36147aa07dafが割り当てられています。   Scientific Committee on Antarctic Research によって承認されたデータ パブリッシャーとして GBIF に登録されているSCAR - Microbial Antarctic Resource System が、このリソースをパブリッシュしました。

キーワード

Metadata

連絡先

リソースを作成した人:

Donnabella Lacap-bugler
Auckland University of Technology Auckland NZ
Kevin Lee
Auckland University of Technology Auckland NZ
Stephen Archer
Auckland University of Technology Auckland NZ
Len Gillman
Auckland University of Technology Auckland NZ
Maggie Lau
Princeton University Princeton US
Sebastian Leuzinger
Auckland University of Technology Auckland NZ
Charles Lee
University of Waikato Hamilton NZ
Teruya Maki
Kanazawa University Kanazawa JP
Christoffer McKay
National Aeronautics and Space Administration Ames Research Center Moffett Field US
John Perrott
Auckland University of Technology Auckland NZ
Asunción de los Rios-Murillo
Museo Nacional de Ciencias Naturales Madrid ES
Kimberley Warren-Rhodes
Kanazawa University Kanazawa JP
David Hopkins
The Royal Agricultural University Cirencester GB
Stephen Pointing
Auckland University of Technology Auckland NZ

リソースに関する質問に答えることができる人:

Donnabella Lacap-bugler
Auckland University of Technology Auckland NZ

メタデータを記載した人:

Maxime Sweetlove
Research assistent
Royal Belgian Institute for Natural Sciences Rue Vautier 29 1000 Brussels

他に、リソースに関連付けられていた人:

データ利用者

地理的範囲

Tibetan Plateau, China; Taklimakan Desert, China; Devon Island (Arctic), Canada; McMurdo Dry Valleys, Antarctica

座標(緯度経度) 南 西 [-77.41, 88.616], 北 東 [42.715, 161.193]

生物分類学的範囲

Bacteria (16S ssu rRNA) and Cyanobacteria (nifH)

Domain  Bacteria (Bacteria)
Phylum  Cyanobacteria (Cyanobacteria)

プロジェクトデータ

説明がありません

タイトル Global biogeography of desert cyanobacteria
ファンデイング The research was funded by the NASA Astrobiology Science and Technology for Exploring Planets (ASTEP) program and the Institute for Applied Ecology New Zealand (www.aenz.aut.ac.nz).

プロジェクトに携わる要員:

Donnabella Lacab-bugler

収集方法

Colonized quartz stones were retrieved by hand (using isopropyl alcohol surface sterilized latex gloves) and loose soil particles gently removed with a sterile (autoclaved) paintbrush. Samples were then stored in sterile Whirlpak bags (Nasco) at -20°C in the field and in transit, and subsequently stored frozen at -80°C in the laboratory until processed.

Study Extent Hypolithic communities were recovered from quartz substrate in desert pavement on the Tibetan Plateau (China), the Taklimakan Desert (China), Devon Island (Canada) and the McMurdo Dry Valleys (Antarctica).

Method step description:

  1. Amplification of 16S rRNA genes was achieved using primer pair 341F and 907R with PCR conditions involving an initial denaturation time of 5min; 30 cycles at 95°C for 1 min, 55°C for 1 min, 72°C for 1 min, and a final extension at 72°C for 10 min. Positive and negative controls were run for every PCR. For each amplicon library purification was carried out with Agencourt AMPure XP Bead (Beckman Coulter, CA, USA) according to manufacturer’s instructions. The libraries were quantified with Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen Life Technologies, NY, USA) using FLUOstar OPTIMA F fluorometer (BMG Labtech GmbH, Offenburg, Germany) and library quality was assessed with the FlashGel System (Lonza Group Ltd., Basel, Switzerland).
  2. Emulsion-PCR was carried out with GS Junior Titanium emPCR Kit (Lib-L, 454 Life Sciences Corp., CT, USA) according to the emPCR Amplification Method Manual – Lib-L, Single-Prep. The sequencing reaction was carried out with the GS Junior Titanium Sequencing Kit and GS Junior Titanium PicoTiterPlate Kit (454 Life Sciences Corp.) according to the manufacturer’s instructions. The sequencing run was conducted in 200 cycles.

書誌情報の引用

  1. Lacap-Bugler, D. C., Lee, K. K., Archer, S., Gillman, L. N., Lau, M. C., Leuzinger, S., ... & de los Rios-Murillo, A. (2017). Global diversity of desert hypolithic cyanobacteria. Frontiers in microbiology, 8, 867.

追加のメタデータ

代替識別子 914f39c7-7bb2-4e77-a4ae-36147aa07daf
https://ipt.biodiversity.aq/resource?r=desert_cyanobacteria