Global biogeography of desert cyanobacteria

Versão mais recente published by SCAR - Microbial Antarctic Resource System on mar 19, 2019 SCAR - Microbial Antarctic Resource System
Publication date:
19 de Março de 2019
Licença:
CC-BY 4.0

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Descrição

Amplicon sequencing dataset (454 pyrosequencing) of Bacteria (16S ssu rRNA) and Cyanobacteria (nifH) in cold desert quartz rocks

Versões

A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.

Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Lacap-bugler D, Lee K, Archer S, Gillman L, Lau M, Leuzinger S, Lee C, Maki T, McKay C, Perrott J, de los Rios-Murillo A, Warren-Rhodes K, Hopkins D, Pointing S (2018): Global biogeography of desert cyanobacteria. v1.4. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=desert_cyanobacteria&v=1.4

Direitos

Pesquisadores devem respeitar a seguinte declaração de direitos:

O editor e o detentor dos direitos deste trabalho é SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 914f39c7-7bb2-4e77-a4ae-36147aa07daf.  SCAR - Microbial Antarctic Resource System publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por Scientific Committee on Antarctic Research.

Palavras-chave

Metadata

Contatos

Donnabella Lacap-bugler
  • Originador
  • Ponto De Contato
Auckland University of Technology
Auckland
NZ
Kevin Lee
  • Originador
Auckland University of Technology
Auckland
NZ
Stephen Archer
  • Originador
Auckland University of Technology
Auckland
NZ
Len Gillman
  • Originador
Auckland University of Technology
Auckland
NZ
Maggie Lau
  • Originador
Princeton University
Princeton
US
Sebastian Leuzinger
  • Originador
Auckland University of Technology
Auckland
NZ
Charles Lee
  • Originador
University of Waikato
Hamilton
NZ
Teruya Maki
  • Originador
Kanazawa University
Kanazawa
JP
Christoffer McKay
  • Originador
National Aeronautics and Space Administration Ames Research Center
Moffett Field
US
John Perrott
  • Originador
Auckland University of Technology
Auckland
NZ
Asunción de los Rios-Murillo
  • Originador
Museo Nacional de Ciencias Naturales
Madrid
ES
Kimberley Warren-Rhodes
  • Originador
Kanazawa University
Kanazawa
JP
David Hopkins
  • Originador
The Royal Agricultural University
Cirencester
GB
Stephen Pointing
  • Originador
Auckland University of Technology
Auckland
NZ
Maxime Sweetlove
  • Provedor Dos Metadados
Research assistent
Royal Belgian Institute for Natural Sciences
Rue Vautier 29
1000 Brussels

Cobertura Geográfica

Tibetan Plateau, China; Taklimakan Desert, China; Devon Island (Arctic), Canada; McMurdo Dry Valleys, Antarctica

Coordenadas delimitadoras Sul Oeste [-77,41, 88,616], Norte Leste [42,715, 161,193]

Cobertura Taxonômica

Bacteria (16S ssu rRNA) and Cyanobacteria (nifH)

Domínio Bacteria (Bacteria)
Filo Cyanobacteria (Cyanobacteria)

Dados Sobre o Projeto

Nenhuma descrição disponível

Título Global biogeography of desert cyanobacteria
Financiamento The research was funded by the NASA Astrobiology Science and Technology for Exploring Planets (ASTEP) program and the Institute for Applied Ecology New Zealand (www.aenz.aut.ac.nz).

O pessoal envolvido no projeto:

Donnabella Lacab-bugler

Métodos de Amostragem

Colonized quartz stones were retrieved by hand (using isopropyl alcohol surface sterilized latex gloves) and loose soil particles gently removed with a sterile (autoclaved) paintbrush. Samples were then stored in sterile Whirlpak bags (Nasco) at -20°C in the field and in transit, and subsequently stored frozen at -80°C in the laboratory until processed.

Área de Estudo Hypolithic communities were recovered from quartz substrate in desert pavement on the Tibetan Plateau (China), the Taklimakan Desert (China), Devon Island (Canada) and the McMurdo Dry Valleys (Antarctica).

Descrição dos passos do método:

  1. Amplification of 16S rRNA genes was achieved using primer pair 341F and 907R with PCR conditions involving an initial denaturation time of 5min; 30 cycles at 95°C for 1 min, 55°C for 1 min, 72°C for 1 min, and a final extension at 72°C for 10 min. Positive and negative controls were run for every PCR. For each amplicon library purification was carried out with Agencourt AMPure XP Bead (Beckman Coulter, CA, USA) according to manufacturer’s instructions. The libraries were quantified with Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen Life Technologies, NY, USA) using FLUOstar OPTIMA F fluorometer (BMG Labtech GmbH, Offenburg, Germany) and library quality was assessed with the FlashGel System (Lonza Group Ltd., Basel, Switzerland).
  2. Emulsion-PCR was carried out with GS Junior Titanium emPCR Kit (Lib-L, 454 Life Sciences Corp., CT, USA) according to the emPCR Amplification Method Manual – Lib-L, Single-Prep. The sequencing reaction was carried out with the GS Junior Titanium Sequencing Kit and GS Junior Titanium PicoTiterPlate Kit (454 Life Sciences Corp.) according to the manufacturer’s instructions. The sequencing run was conducted in 200 cycles.

Citações bibliográficas

  1. Lacap-Bugler, D. C., Lee, K. K., Archer, S., Gillman, L. N., Lau, M. C., Leuzinger, S., ... & de los Rios-Murillo, A. (2017). Global diversity of desert hypolithic cyanobacteria. Frontiers in microbiology, 8, 867.

Metadados Adicionais

Identificadores alternativos 914f39c7-7bb2-4e77-a4ae-36147aa07daf
https://ipt.biodiversity.aq/resource?r=desert_cyanobacteria