Fungal communities (ITS) in Antarctic brines.

Última versión Publicado por SCAR - Microbial Antarctic Resource System en Mar 19, 2019 SCAR - Microbial Antarctic Resource System

Amplicon sequencing dataset (Illumina MiSeq) of Fungal microbes (ITS marker gene) in two distinct layers (separated by 12cm of ice) of liquid high salinity brines of one lake in the Tarmac Flat area (In the North of McMurdo Dry Valley, Antarctica).

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¿Cómo referenciar?

Los usuarios deben citar este trabajo de la siguiente manera:

Luigimaria B, Sannino C, Selbmann L, Battitel D, Zucconi L, Azzaro M, Turchetti B, Buzzini P, Gugliemin M (2019): Fungal communities (ITS) in Antarctic brines.. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=fungal_its_communities_in_antarctic_brines&v=1.1

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El publicador y propietario de los derechos de este trabajo es SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

Registro GBIF

Este recurso ha sido registrado en GBIF con el siguiente UUID: 50dc522a-3200-454c-b0ed-240b0e247923.  SCAR - Microbial Antarctic Resource System publica este recurso, y está registrado en GBIF como un publicador de datos avalado por Scientific Committee on Antarctic Research.

Palabras Clave

Metadata

Contactos

¿Quién creó el recurso?:

Borruso Luigimaria
University of Bozen-Bolzano Bozen-Bolzano IT
Ciro Sannino
University of Perugia Perugia IT
Laura Selbmann
University of Tuscia Viterbo IT
Dario Battitel
University Ca’ Foscari Venice IT
Laura Zucconi
University of Tuscia Viterbo IT
Maurizio Azzaro
National Research Council Messina IT
Benedetta Turchetti
University of Perugia Perugia IT
Pietro Buzzini
University of Perugia Perugia IT
Mauro Gugliemin
Insubria University Varese IT

¿Quién puede resolver dudas acerca del recurso?:

Borruso Luigimaria
University of Bozen-Bolzano Bozen-Bolzano IT
Pietro Buzzini
University of Perugia Perugia IT

¿Quién documentó los metadatos?:

Maxime Sweetlove
Research assistent
Royal Belgian Istitute for Natural Sciences Rue Vautier 29 1000 Brussels BE

¿Quién más está asociado con el recurso?:

Usuario

Cobertura Geográfica

Flat Tarn Area, McMurdo Dry Valleys, Victoria land Antarctica

Coordenadas límite Latitud Mínima Longitud Mínima [-75.667, 162.5], Latitud Máxima Longitud Máxima [-75.667, 162.5]

Cobertura Taxonómica

Fungi, ITS2 marker gene

Filo  Fungi (Fungi)

Cobertura Temporal

Fecha Inicial 2014-01-01

Datos del Proyecto

No hay descripción disponible

Título Fungal communities (ITS) in Antarctic brines.
Fuentes de Financiación This research was supported by grants from the National Antarctic Research Program (PNRA), Italian Ministry of Education and Research (Research Project PNRA AZ/1.05) and from the National Antarctic Museum (MNA).

Personas asociadas al proyecto:

Borruso Luigimaria

Métodos de Muestreo

In the studied lake (280 m long and 100 m wide, maximum depth around 6 m) the brines were located only within a deep trough in correspondence of which a pingo like feature (PLF) occurs. PLF is a frost mound that intruded the lake ice surface reaching a maximum height of 45 cm and extending within an area of approximately of 500 m2. Below that mound, brines have been preliminarily identified using GPR data; they were then reached and sampled through a 51 mm diameter borehole that was drilled in the center of the frost mound using a semi-portable core auger. In particular, the first pocket of liquid brine (TF1) was found between 3.78 m, and 3.98 m. TF1 was separated by the second pocket of liquid brine (TF2) (0.84 m thick) by only a 12 cm layer of ice (containing some organic material inclusions). Below TF2 additional frozen sediments rich in organic content occurred between 4.94 m and the bottom of the borehole (5.68 m). Both brines were collected in sterile Pyrex bottles using a peristaltic pump and sterile tubing. After collections, the brines were stored at −20 °C in the dark at Mario Zucchelli Station (MZS) prior to their delivery to laboratories for chemical and microbiological analyses.

Área de Estudio Three replicate samples were taken from two separate brine layers in a lake in Tarn Flat area (75°4′S 162°30′E), an ice-free area (ca. 100 km2) in the north to the McMurdo Dry Valley in Victoria Land.

Descripción de la metodología paso a paso:

  1. Total DNA from brines samples was aseptically extracted using Power Water DNA Isolation Kit (Qiagen, Germany) following the operating instructions. Prior to DNA extraction, the brines were thawed at 4 °C and aseptically filtered in order to collect the biomass present in each brine on sterile cellulose acetate filter (cutoff = pore size 0.2 µm Sartorius Stedim, Biotech, Germany). The quality and quantity of DNA extracted was determined by using QuBit 2.0 Fluorometer Assay (Life Technologies Corporation) and by NanoDrop 2000 c spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). Three replicates for each brine were run on Illumina MiSeq.
  2. Fungal internal transcribed spacer region 2 (ITS2) was amplified using IlluAdp_ITS31_NeXTf 5′-CATCGATGAAGAACGCAG-3′ and IlluAdp_ITS4_NeXTr5′-TCCTCCGCTTATTGATATGC-3′60. The PCR products were sequenced using the Illumina MiSeq platforms, following the standard protocols of the company STAB Vida Lda. (Caparica, Portugal).

Referencias Bibliográficas

  1. Forte, E., Dalle Fratte, M., Azzaro, M., & Guglielmin, M. (2016). Pressurized brines in continental Antarctica as a possible analogue of Mars. Scientific reports, 6, 33158.
  2. Borruso, L., Sannino, C., Selbmann, L., Battistel, D., Zucconi, L., Azzaro, M., ... & Guglielmin, M. (2018). A thin ice layer segregates two distinct fungal communities in Antarctic brines from Tarn Flat (Northern Victoria Land). Scientific reports, 8.

Metadatos Adicionales

Identificadores Alternativos 50dc522a-3200-454c-b0ed-240b0e247923
https://ipt.biodiversity.aq/resource?r=fungal_its_communities_in_antarctic_brines