Geothermal Fumarole Subsurface Mt. Erebus, Antarctica

Versão mais recente publicado por SCAR - Microbial Antarctic Resource System em Oct 19, 2015 SCAR - Microbial Antarctic Resource System

The Tramway Ridge geothermal site on Mt. Erebus in Antarctica, is the most geographically isolated geothermal site on earth. This makes it an excellent system for studies of microbial speciation, biogeography, and evolution.

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Os dados deste recurso de ocorrência foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 40 registros.

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Versões

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Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Herbold, CW; Lee, CK; McDonald, IR; Cary, SC; Evidence of global-scale aeolian dispersal and endemism in isolated geothermal microbial communities of Antarctica; Nature Comms (2014); 5

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: d2744050-427f-4b3f-824d-e96b7bd26368.  SCAR - Microbial Antarctic Resource System publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por Scientific Committee on Antarctic Research.

Palavras-chave

Occurrence; Observation

Contatos

Quem criou esse recurso:

Craig Herbold
Group Leader
Division of Microbial Ecology (DoME), University of Vienna Althanstrasse 14 1090 Vienna AT
http://www.microbial-ecology.net/

Quem pode responder a perguntas sobre o recurso:

S. Craig Cary
Professor
University of Waikato Private Bag 3105 3105 Hamilton NZ
http://www.ictar.aq/ictar_leadership.cfm?staff_id=1&page_obj_id=246&obj_list=1

Quem preencher os metadados:

Craig Herbold
Group Leader
Division of Microbial Ecology (DoME), University of Vienna Althanstrasse 14 1090 Vienna AT
http://www.microbial-ecology.net/

Cobertura Geográfica

ASPA 130 - Tramway Ridge Specially Protected Area on the northwest summit of Mt. Erebus, Antarctica

Coordenadas delimitadoras Sul Oeste [-77.519, 167.106], Norte Leste [-77.517, 167.114]

Cobertura Temporal

Data Inicial 2009-02-09

Dados Sobre o Projeto

Nenhuma descrição disponível

Título Life at the extreme: resolving the genetic basis of microbial endemism in the super-heated soils of Mt Erebus, Antarctica
Financiamento Financial support was provided by grant UOW0802 from the New Zealand Marsden Fund to SCC and IRM and a CRE award from the National Geographic Society to SCC. Antarctic logistic support for Event K-023 was provided by Antarctica New Zealand.
Descrição da Área de Estudo The summit of Mt. Erebus features several high-elevation geothermal features that are separated from similar features at Mts. Melbourne and Rittman by 350-400 km. Warm fumarolic ground and ice towers on the flanks of Mt. Erebus passively emit steam and CO2 that are believed to have magmatic origins(Wardell et al., 2003). The lower end of Tramway Ridge, located approximately 1.5 km NW of the main crater of Erebus, at an elevation between 3350 and 3400 m, is an extensive warm fumarolic area protected by international treaty as a site of particular biological interest (ASPA 130 Management Plan). At Tramway Ridge, unique communities of photoautotrophic organisms (mosses and cyanobacterial mats) surround fumaroles that reach and maintain year round surface temperatures of 60-65°C, have a neutral to mildly alkaline pH, and are characterized by steep lateral pH and temperature gradients (Broady, 1984; Hudson et al., 1989; Soo et al., 2009).
Descrição do Design The current study aimed to fully characterize the distribution of microbial communities inhabiting the 60-65°C sites of the CO2-emitting fumaroles located at Tramway Ridge, and to identify the set of organisms specifically associated with the subsurface. To meet these objectives, a mixed amplicon /metagenomic pyrosequencing-based approach was employed. Correlated relative abundance, as calculated from 16S rRNA gene amplicon libraries was used to identify sets of organisms that occupy shared niches. A pooled shotgun-metagenomic dataset was then used to both verify the abundance of dominant organisms and reconstruct whole 16S rRNA genes, allowing more comprehensive phylogenetic analyses.

O pessoal envolvido no projeto:

Pesquisador Principal
S. Craig Cary

Métodos de Amostragem

Sediment samples were collected within the Tramway Ridge Antarctic Specially Protected Area (ASPA 130) in February 2009 from two sites (site A: 77° 31.103' S, 167° 6.682' S and site B: 77° 31.106' S, 167° 6.668' E). All suggested sterilization protocols for entering into this protected site were adhered to, following the ASPA 130 Management Plan (http://www.scar.org/publications/bulletins/151/aspa130.html). Sites were chosen based on measuring a surface temperature of 65°C with a stainless steel Checktemp1 temperature probe (Hanna Instruments, Rhode Island, USA), sterilized with 70% ethanol immediately prior to each use. Surface "crust" was set aside prior to collecting samples. Samples were collected by aseptically removing the top 2 cm of sediment in an approximately 25cm2 area. Sediment was placed into a fresh 50 mL Falcon tube. Sampling continued with the collection of a second (2-4 cm depth) and third (4-8 cm depth) layer following the same procedures. Temperature measurements were repeated for each layer sampled. All samples were immediately frozen and maintained at -80°C in the laboratory until analysed.

Área de Estudo The study encompassed two locations in the permitted area of the Tramway Ridge ASPA 130. http://www.nature.com/ncomms/2014/140520/ncomms4875/fig_tab/ncomms4875_F1.html
Controle de Qualidade Sampling conditions resulted in a high probability of cross-contamination between sampling depths. We used standard quality filtering for our 454 datasets, including denoising and chimera removal with AmpliconNoise. Sequence abundances, as inferred from library abundance, were used to cluster individual OTUs into correlated clusters which defined whether a given sequence was likely of surface or subsurface origin. Shotgun metagenomics was utilized to extract full-length 16S rRNA sequences for high-resolution phylogenetic analysis.

Descrição dos passos do método:

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Dados de Coleção

Nome da Coleção Geothermal fumarole subsurface microbial communities from Mt. Erebus, Antarctica
Identificador da Coleção http://www.ncbi.nlm.nih.gov/nuccore/KF923316,KF923317,KF923318,KF923319,KF923320,KF923321,KF923322,KF923323,KF923324,KF923325,KF923326,KF923327
Identificador da Coleção Parental Not Applicable
Métodos de preservação do espécime Congelado

Metadados Adicionais

Identificadores alternativos d2744050-427f-4b3f-824d-e96b7bd26368
http://ipt.biodiversity.aq/resource.do?r=geothermal_fumarole_subsurface_mt_erebus_antarctica