Inter-Valley Soil Comparative Survey of the McMurdo Dry Valleys

Última versión Publicado por SCAR - Microbial Antarctic Resource System en Mar 19, 2019 SCAR - Microbial Antarctic Resource System

Recent applications of molecular genetics to edaphic microbial communities of the McMurdo Dry Valleys and elsewhere have rejected a long-held belief that Antarctic soils contain extremely limited microbial diversity. The Inter-Valley Soil Comparative Survey aims to elucidate the factors shaping these unique microbial communities and their biogeography by integrating molecular genetic approaches with biogeochemical analyses. Although the microbial communities of Dry Valley soils may be complex, there is little doubt that the ecosystem’s food web is relatively simple, and evidence suggests that physicochemical conditions may have the dominant role in shaping microbial communities. To examine this hypothesis, bacterial communities from representative soil samples collected in four geographically disparate Dry Valleys were analyzed using molecular genetic tools, including pyrosequencing of 16S rRNA gene PCR amplicons. Results show that the four communities are structurally and phylogenetically distinct, and possess significantly different levels of diversity. Strikingly, only 2 of 214 phylotypes were found in all four valleys, challenging a widespread assumption that the microbiota of the Dry Valleys is composed of a few cosmopolitan species. Analysis of soil geochemical properties indicated that salt content, alongside altitude and Cu2+, was significantly correlated with differences in microbial communities. Our results indicate that the microbial ecology of Dry Valley soils is highly localized and that physicochemical factors potentially have major roles in shaping the microbiology of ice-free areas of Antarctica. These findings hint at links between Dry Valley glacial geomorphology and microbial ecology, and raise previously unrecognized issues related to environmental management of this unique ecosystem.

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Versiones

La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.

¿Cómo referenciar?

Los usuarios deben citar este trabajo de la siguiente manera:

Lee CK, Barbier BA, Bottos EM, McDonald IR, Cary SC (2012) The Inter-Valley Soil Comparative Survey: the ecology of Dry Valley edaphic microbial communities. ISME J 6: 1046–1057. doi:10.1038/ismej.2011.170.

Derechos

Los usuarios deben respetar los siguientes derechos de uso:

El publicador y propietario de los derechos de este trabajo es SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License.

Registro GBIF

Este recurso ha sido registrado en GBIF con el siguiente UUID: cca539d1-3e43-49bb-8e1f-8c1bda934a8c.  SCAR - Microbial Antarctic Resource System publica este recurso, y está registrado en GBIF como un publicador de datos avalado por Scientific Committee on Antarctic Research.

Palabras Clave

Bacteria; Cyanobacteria; Fungi; Terrestrial Antarctica; McMurdo Dry Valleys; 16S rRNA; Soil; Metadata

Contactos

¿Quién creó el recurso?:

Charles Lee
Senior Lecturer
University of Waikato Private Bag 3105 3240 Hamilton NZ +64 7 838 4241
http://www.ictar.aq

¿Quién puede resolver dudas acerca del recurso?:

Charles Lee
Senior Lecturer
University of Waikato Private Bag 3105 3240 Hamilton NZ +64 7 838 4241
http://www.ictar.aq

¿Quién documentó los metadatos?:

Charles Lee
Senior Lecturer
University of Waikato Private Bag 3105 3240 Hamilton NZ +64 7 838 4241
http://www.ictar.aq

¿Quién más está asociado con el recurso?:

Autor
Charles Lee
Research Fellow
International Center for Terrestrial Antarctic Research Private Bag 3105 3240 Hamilton NZ +64 7 838 4241
http://www.ictar.aq
Investigador Principal
Craig Cary
Professor
International Center for Terrestrial Antarctic Research Private Bag 3105 3240 Hamilton NZ +64 7 838 4593
http://www.ictar.aq

Cobertura Geográfica

The McMurdo Dry Valleys

Coordenadas límite Latitud Mínima Longitud Mínima [-78.091, 160.764], Latitud Máxima Longitud Máxima [-76.912, 163.809]

Cobertura Taxonómica

Molecular surveys of bacterial (16S rRNA) and fungal (ITS) marker genes

Dominio  Bacteria
Reino  Fungi

Cobertura Temporal

Fecha Inicial / Fecha Final 2006-12-01 / 2006-12-31
Fecha Inicial / Fecha Final 2008-01-01 / 2008-01-31
Fecha Inicial / Fecha Final 2010-11-01 / 2010-11-30

Datos del Proyecto

No hay descripción disponible

Título Inter-Valley Soil Comparative Survey of the McMurdo Dry Valleys
Fuentes de Financiación Funding was provided by the New Zealand Foundation for Research, Science and Technology (FRST) (UOWX0715) and the New Zealand Marsden Fund (UOW1003). Logistics support was provided by Antarctica New Zealand.
Descripción del Área de Estudio Soil was collected at six different sites in the McMurdo Dry Valleys: Alatna Valley, Battleship Promontory, Upper Wright Valley, Beacon Valley, University Valley, and Miers Valley.

Personas asociadas al proyecto:

Investigador Principal
Craig Cary

Métodos de Muestreo

Sampling sites were all located on a south facing, 0–20°slope. An intersection was made by two 50 m transects, with the intersection in the middle being the central sampling point (X or C). Four sampling points around the central point were marked (A–D with A being the southernmost point and the remaining points in an anti-clockwise order, or N, E, S, W). Five scoops of the top 2 cm of soil were collected and homogenized at each identified (1 m2) sampling point after pavement pebbles were removed. Samples were stored in sterile Whirl-Pak (Nasco International, Fort Atkinson, WI, USA) at −20 °C until returned to New Zealand, where they were stored at −80 °C until analysis.

Área de Estudio See Geographic Coverage
Control de Calidad 454 pyrosquencing flowgrams were denoised using AmpliconNoise, including a SeqNoise step to remove PCR errors and a Perseus step to remove PCR chimeras. See Lee et al 2012 and Dreesens et al 2014 for details.

Descripción de la metodología paso a paso:

  1. Two MICROBIAL_SEQUENCE_SET description files describing X data sets was uploaded to the IPT. X MIMARKS data files were uploaded to the IPT.

Datos de la Colección

Nombre de la Colección Soil Samples from McMurdo Dry Valleys, Antarctica
Identificador de la Colección N/A
Identificador de la Colección Parental N/A
Métodos de preservación de los ejemplares Congelado

Referencias Bibliográficas

  1. Dreesens L, Lee CK, Cary SC (2014) The Distribution and Identity of Edaphic Fungi in the McMurdo Dry Valleys. Biology 3: 466–483. doi:10.3390/biology3030466. 10.3390/biology3030466

Metadatos Adicionales

Identificadores Alternativos cca539d1-3e43-49bb-8e1f-8c1bda934a8c
https://ipt.biodiversity.aq/resource?r=ictar_ivscs