Microbes (Eukaryotes and Archaea) in sea water from Fildes Peninsula (King George Island, Antarctica)

Dernière version Publié par SCAR - Microbial Antarctic Resource System le mars 19, 2019 SCAR - Microbial Antarctic Resource System
Date de publication:
19 mars 2019
Licence:
CC-BY 4.0

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Description

Amplicon sequencing dataset (Illumina MiSeq) of microbial Eukaryotes (18S ssu rRNA gene), and Archaea in sea water samples taken during the 29th Chinese Antarctic scientific expedition in 2013 at Greatwall cove and Ardley cove, Fildes Peninsula (King George Island, Antarctica).

Versions

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Comment citer

Les chercheurs doivent citer cette ressource comme suit:

Luo W, Li H, Gao S, Yu Y, Lin L, Zeng T (2019): Microbes (Eukaryotes and Archaea) in sea water from Fildes Peninsula (King George Island, Antarctica). v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbes_fildes_peninsula_antarctica&v=1.2

Droits

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L’éditeur et détenteur des droits de cette ressource est SCAR - Microbial Antarctic Resource System. Ce travail est sous licence Creative Commons Attribution (CC-BY) 4.0.

Enregistrement GBIF

Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : a4af5ceb-4035-49f5-b41a-bb548307b4f8.  SCAR - Microbial Antarctic Resource System publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du Scientific Committee on Antarctic Research.

Mots-clé

Metadata

Contacts

Wei Luo
  • Créateur
  • Personne De Contact
Polar Research Institute of China
Shanghai
CN
Huirong Li
  • Créateur
Polar Research Institute of China
Shanghai
CN
Shengquan Gao
  • Créateur
Second Institute of Oceanography
Hangzhou
CN
Yong Yu
  • Créateur
Polar Research Institute of China
Shanghai
CN
Ling Lin
  • Créateur
Polar Research Institute of China
Shanghai
CN
Tinxin Zeng
  • Créateur
Polar Research Institute of China
Shanghai
CN
Maxime Sweetlove
  • Fournisseur Des Métadonnées
Research assistent
Royal Belgian Institute of Natural Sciences
Rue Vautier 29
1000 Brussels

Couverture géographique

Fildes Peninsula, King George Island, Antarctica

Enveloppe géographique Sud Ouest [-62,2, -58,9], Nord Est [-62,2, -58,9]

Couverture taxonomique

microbial Eukaryotes were sampled based on marker gene amplification (18S ssu rRNA gene)

Domain Eukaryota (Eukaryotes)

Archaea were sampled based on marker gene amplification

Domain Archaea (Archaea)

Couverture temporelle

Date de début / Date de fin 2013-01-17 / 2013-01-23

Données sur le projet

Pas de description disponible

Titre Microbes (Eukaryotes and Archaea) in sea water from Fildes Peninsula (King George Island, Antarctica)
Financement This work was supported by the National High-Tech Research and Development Program of China (Grant Nos. 2012AA021706, 2013AA065805), National Natural Science Foundation of China (No. 41376191), Chinese Polar Environment Comprehensive Investigation and Assessment Program (CHINARE2014-02-01), and Shanghai Rising-Star Program (11QA1407300).

Les personnes impliquées dans le projet:

Wei Luo

Méthodes d'échantillonnage

1 L of surface sea water from each station was collected and prefiltered through a 20-µm mesh sieve to remove most of the mesozooplankton and large particles, and then directly filtered through a 0.2-µm pore size nucleopore membrane filter (Whatman). The filters were frozen at −80 °C in cetyltrimethylammonium bromide (CTAB) buffer until laboratory experiments were carried out.

Etendue de l'étude Samples were taken in January 2013, during the 29th Chinese National Antarctic Research Expedition at Greatwall Cove and Ardley Cove (king George Island, Antarctica)

Description des étapes de la méthode:

  1. DNA extraction was performed as described by Luo et al. (2009).
  2. Polymerase chain reaction (PCR) was performed using primers with barcodes flanking the hypervariable V4 region of the 18S rRNA gene: 3NDf with the reverse primer V4_euk_R2. PCR was conducted in 20 μL reactions with 0.2 μM each of the primers, ~10 ng of template DNA, 1 × PCR buffer, and 2.5 U of Pfu DNA Polymerase (Promega, USA). The amplification programme consisted of an initial denaturation step at 95 °C for 2 min, followed by 30 cycles at 95 °C for 30 s, 55 °C for 30 s, and 72 °C for 30 s, and a final extension of 72 °C for 5 min. PCR products were pooled and purified using a DNA gel extraction kit (Axygen, Hangzhou, China). The DNA concentration of each PCR product was determined using a Quant-iT PicoGreen double-stranded DNA assay (Invitrogen, Germany) and was quality controlled on a TBS-380 Mini-Fluorometer (Turner Biosystems, Sunnyvale, CA, USA). Finally, amplicons of all samples were pooled in equimolar concentrations.
  3. 18S rRNA amplification and sequencing on the Illumina MiSeq2000 were done by following the standard protocols of Earth Microbiome Project (EMP) (Caporaso et al. 2012).

Citations bibliographiques

  1. Luo, W., Li, H., Gao, S., Yu, Y., Lin, L., & Zeng, Y. (2016). Molecular diversity of microbial eukaryotes in sea water from Fildes Peninsula, King George Island, Antarctica. Polar Biology, 39(4), 605-616. https://doi.org/10.1007/s00300-015-1815-8

Métadonnées additionnelles

Identifiants alternatifs a4af5ceb-4035-49f5-b41a-bb548307b4f8
https://ipt.biodiversity.aq/resource?r=microbes_fildes_peninsula_antarctica