Microbes (Eukaryotes and Archaea) in sea water from Fildes Peninsula (King George Island, Antarctica)

Versão mais recente published by SCAR - Microbial Antarctic Resource System on mar 19, 2019 SCAR - Microbial Antarctic Resource System
Publication date:
19 de Março de 2019
Licença:
CC-BY 4.0

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Descrição

Amplicon sequencing dataset (Illumina MiSeq) of microbial Eukaryotes (18S ssu rRNA gene), and Archaea in sea water samples taken during the 29th Chinese Antarctic scientific expedition in 2013 at Greatwall cove and Ardley cove, Fildes Peninsula (King George Island, Antarctica).

Versões

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Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Luo W, Li H, Gao S, Yu Y, Lin L, Zeng T (2019): Microbes (Eukaryotes and Archaea) in sea water from Fildes Peninsula (King George Island, Antarctica). v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbes_fildes_peninsula_antarctica&v=1.2

Direitos

Pesquisadores devem respeitar a seguinte declaração de direitos:

O editor e o detentor dos direitos deste trabalho é SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: a4af5ceb-4035-49f5-b41a-bb548307b4f8.  SCAR - Microbial Antarctic Resource System publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por Scientific Committee on Antarctic Research.

Palavras-chave

Metadata

Contatos

Wei Luo
  • Originador
  • Ponto De Contato
Polar Research Institute of China
Shanghai
CN
Huirong Li
  • Originador
Polar Research Institute of China
Shanghai
CN
Shengquan Gao
  • Originador
Second Institute of Oceanography
Hangzhou
CN
Yong Yu
  • Originador
Polar Research Institute of China
Shanghai
CN
Ling Lin
  • Originador
Polar Research Institute of China
Shanghai
CN
Tinxin Zeng
  • Originador
Polar Research Institute of China
Shanghai
CN
Maxime Sweetlove
  • Provedor Dos Metadados
Research assistent
Royal Belgian Institute of Natural Sciences
Rue Vautier 29
1000 Brussels

Cobertura Geográfica

Fildes Peninsula, King George Island, Antarctica

Coordenadas delimitadoras Sul Oeste [-62,2, -58,9], Norte Leste [-62,2, -58,9]

Cobertura Taxonômica

microbial Eukaryotes were sampled based on marker gene amplification (18S ssu rRNA gene)

Domínio Eukaryota (Eukaryotes)

Archaea were sampled based on marker gene amplification

Domínio Archaea (Archaea)

Cobertura Temporal

Data Inicial / Data final 2013-01-17 / 2013-01-23

Dados Sobre o Projeto

Nenhuma descrição disponível

Título Microbes (Eukaryotes and Archaea) in sea water from Fildes Peninsula (King George Island, Antarctica)
Financiamento This work was supported by the National High-Tech Research and Development Program of China (Grant Nos. 2012AA021706, 2013AA065805), National Natural Science Foundation of China (No. 41376191), Chinese Polar Environment Comprehensive Investigation and Assessment Program (CHINARE2014-02-01), and Shanghai Rising-Star Program (11QA1407300).

O pessoal envolvido no projeto:

Wei Luo

Métodos de Amostragem

1 L of surface sea water from each station was collected and prefiltered through a 20-µm mesh sieve to remove most of the mesozooplankton and large particles, and then directly filtered through a 0.2-µm pore size nucleopore membrane filter (Whatman). The filters were frozen at −80 °C in cetyltrimethylammonium bromide (CTAB) buffer until laboratory experiments were carried out.

Área de Estudo Samples were taken in January 2013, during the 29th Chinese National Antarctic Research Expedition at Greatwall Cove and Ardley Cove (king George Island, Antarctica)

Descrição dos passos do método:

  1. DNA extraction was performed as described by Luo et al. (2009).
  2. Polymerase chain reaction (PCR) was performed using primers with barcodes flanking the hypervariable V4 region of the 18S rRNA gene: 3NDf with the reverse primer V4_euk_R2. PCR was conducted in 20 μL reactions with 0.2 μM each of the primers, ~10 ng of template DNA, 1 × PCR buffer, and 2.5 U of Pfu DNA Polymerase (Promega, USA). The amplification programme consisted of an initial denaturation step at 95 °C for 2 min, followed by 30 cycles at 95 °C for 30 s, 55 °C for 30 s, and 72 °C for 30 s, and a final extension of 72 °C for 5 min. PCR products were pooled and purified using a DNA gel extraction kit (Axygen, Hangzhou, China). The DNA concentration of each PCR product was determined using a Quant-iT PicoGreen double-stranded DNA assay (Invitrogen, Germany) and was quality controlled on a TBS-380 Mini-Fluorometer (Turner Biosystems, Sunnyvale, CA, USA). Finally, amplicons of all samples were pooled in equimolar concentrations.
  3. 18S rRNA amplification and sequencing on the Illumina MiSeq2000 were done by following the standard protocols of Earth Microbiome Project (EMP) (Caporaso et al. 2012).

Citações bibliográficas

  1. Luo, W., Li, H., Gao, S., Yu, Y., Lin, L., & Zeng, Y. (2016). Molecular diversity of microbial eukaryotes in sea water from Fildes Peninsula, King George Island, Antarctica. Polar Biology, 39(4), 605-616. https://doi.org/10.1007/s00300-015-1815-8

Metadados Adicionais

Identificadores alternativos a4af5ceb-4035-49f5-b41a-bb548307b4f8
https://ipt.biodiversity.aq/resource?r=microbes_fildes_peninsula_antarctica