Microbes (Eukaryotes and Archaea) in sea water from Fildes Peninsula (King George Island, Antarctica)

最新版本 published by SCAR - Microbial Antarctic Resource System on 三月 19, 2019 SCAR - Microbial Antarctic Resource System
發布日期:
2019年3月19日
授權條款:
CC-BY 4.0

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說明

Amplicon sequencing dataset (Illumina MiSeq) of microbial Eukaryotes (18S ssu rRNA gene), and Archaea in sea water samples taken during the 29th Chinese Antarctic scientific expedition in 2013 at Greatwall cove and Ardley cove, Fildes Peninsula (King George Island, Antarctica).

版本

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如何引用

研究者應依照以下指示引用此資源。:

Luo W, Li H, Gao S, Yu Y, Lin L, Zeng T (2019): Microbes (Eukaryotes and Archaea) in sea water from Fildes Peninsula (King George Island, Antarctica). v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbes_fildes_peninsula_antarctica&v=1.2

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此資料的發布者及權利單位為 SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF 註冊

此資源已向GBIF註冊,並指定以下之GBIF UUID: a4af5ceb-4035-49f5-b41a-bb548307b4f8。  SCAR - Microbial Antarctic Resource System 發佈此資源,並經由Scientific Committee on Antarctic Research同意向GBIF註冊成為資料發佈者。

關鍵字

Metadata

聯絡資訊

Wei Luo
  • 出處
  • 連絡人
Polar Research Institute of China
Shanghai
CN
Huirong Li
  • 出處
Polar Research Institute of China
Shanghai
CN
Shengquan Gao
  • 出處
Second Institute of Oceanography
Hangzhou
CN
Yong Yu
  • 出處
Polar Research Institute of China
Shanghai
CN
Ling Lin
  • 出處
Polar Research Institute of China
Shanghai
CN
Tinxin Zeng
  • 出處
Polar Research Institute of China
Shanghai
CN
Maxime Sweetlove
  • 元數據提供者
Research assistent
Royal Belgian Institute of Natural Sciences
Rue Vautier 29
1000 Brussels

地理涵蓋範圍

Fildes Peninsula, King George Island, Antarctica

界定座標範圍 緯度南界 經度西界 [-62.2, -58.9], 緯度北界 經度東界 [-62.2, -58.9]

分類群涵蓋範圍

microbial Eukaryotes were sampled based on marker gene amplification (18S ssu rRNA gene)

Domain Eukaryota (Eukaryotes)

Archaea were sampled based on marker gene amplification

Domain Archaea (Archaea)

時間涵蓋範圍

起始日期 / 結束日期 2013-01-17 / 2013-01-23

計畫資料

無相關描述

計畫名稱 Microbes (Eukaryotes and Archaea) in sea water from Fildes Peninsula (King George Island, Antarctica)
經費來源 This work was supported by the National High-Tech Research and Development Program of China (Grant Nos. 2012AA021706, 2013AA065805), National Natural Science Foundation of China (No. 41376191), Chinese Polar Environment Comprehensive Investigation and Assessment Program (CHINARE2014-02-01), and Shanghai Rising-Star Program (11QA1407300).

參與計畫的人員:

Wei Luo

取樣方法

1 L of surface sea water from each station was collected and prefiltered through a 20-µm mesh sieve to remove most of the mesozooplankton and large particles, and then directly filtered through a 0.2-µm pore size nucleopore membrane filter (Whatman). The filters were frozen at −80 °C in cetyltrimethylammonium bromide (CTAB) buffer until laboratory experiments were carried out.

研究範圍 Samples were taken in January 2013, during the 29th Chinese National Antarctic Research Expedition at Greatwall Cove and Ardley Cove (king George Island, Antarctica)

方法步驟描述:

  1. DNA extraction was performed as described by Luo et al. (2009).
  2. Polymerase chain reaction (PCR) was performed using primers with barcodes flanking the hypervariable V4 region of the 18S rRNA gene: 3NDf with the reverse primer V4_euk_R2. PCR was conducted in 20 μL reactions with 0.2 μM each of the primers, ~10 ng of template DNA, 1 × PCR buffer, and 2.5 U of Pfu DNA Polymerase (Promega, USA). The amplification programme consisted of an initial denaturation step at 95 °C for 2 min, followed by 30 cycles at 95 °C for 30 s, 55 °C for 30 s, and 72 °C for 30 s, and a final extension of 72 °C for 5 min. PCR products were pooled and purified using a DNA gel extraction kit (Axygen, Hangzhou, China). The DNA concentration of each PCR product was determined using a Quant-iT PicoGreen double-stranded DNA assay (Invitrogen, Germany) and was quality controlled on a TBS-380 Mini-Fluorometer (Turner Biosystems, Sunnyvale, CA, USA). Finally, amplicons of all samples were pooled in equimolar concentrations.
  3. 18S rRNA amplification and sequencing on the Illumina MiSeq2000 were done by following the standard protocols of Earth Microbiome Project (EMP) (Caporaso et al. 2012).

引用文獻

  1. Luo, W., Li, H., Gao, S., Yu, Y., Lin, L., & Zeng, Y. (2016). Molecular diversity of microbial eukaryotes in sea water from Fildes Peninsula, King George Island, Antarctica. Polar Biology, 39(4), 605-616. https://doi.org/10.1007/s00300-015-1815-8

額外的詮釋資料

替代的識別碼 a4af5ceb-4035-49f5-b41a-bb548307b4f8
https://ipt.biodiversity.aq/resource?r=microbes_fildes_peninsula_antarctica