Microorganisms (Bacteria and Archaea) in the marine cavity beneath the McMurdo Ice Shelf, Antarctica

Dernière version Publié par SCAR - Microbial Antarctic Resource System le Mar 19, 2019 SCAR - Microbial Antarctic Resource System

Amplicon sequencing dataset (Illumina MiSeq) of Bacteria and Archaea (16S) in the marine cavity beneath the McMurdo Ice Shelf, Antarctica

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Vick-Majors T, Achberger A, Santibanez P, Dore J, Hodson T, Michaud A, Christner B, Mikucki J, Skidmore M, Powell R, Adkins P, Brabante C, Mitchell A, Scherer R, Priscu J (2019): Microorganisms (Bacteria and Archaea) in the marine cavity beneath the McMurdo Ice Shelf, Antarctica. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbes_sub_ice_seawater_antarctica&v=1.1

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L’éditeur et détenteur des droits de cette ressource est SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

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Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : 4200a83c-9ff7-43e0-ae09-ef1211847673.  SCAR - Microbial Antarctic Resource System publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du Scientific Committee on Antarctic Research.

Mots-clé

Metadata

Contacts

Personne ayant créé cette ressource:

Trista Vick-Majors
Montana State University Bozeman US
Amanda Achberger
Louisiana State University Baton Rouge US
Pamela Santibanez
Montana State University Bozeman US
John Dore
Montana State University Bozeman US
Timothy Hodson
Northern Illinois University DeKalb US
Alexander Michaud
Montana State University Bozeman US
Brent Christner
Louisiana State University Baton Rouge US
Jill Mikucki
Middlebury College Middlebury US
Mark Skidmore
Montana State University Bozeman US
Ross Powell
Northern Illinois University DeKalb US
Peyton Adkins
Louisiana State University Baton Rouge US
Carlo Brabante
University of Venice Venice IT
Andrew Mitchell
Aberystwyth University Aberystwyth GB
Reed Scherer
Northern Illinois University DeKalb US
John Priscu
Montana State University Bozeman US

Personne pouvant répondre aux questions sur la ressource:

John Priscu
Montana State University Bozeman US

Personne ayant renseigné les métadonnées:

Maxime Sweetlove
Research assistent
Royal Belgian Institute of Natural Science Rue Vautier 29 1000 Brussels

Autres personnes associées à la ressource:

Utilisateur

Couverture géographique

the marine cavity beneath the McMurdo Ice Shelf, Antarctica

Enveloppe géographique Sud Ouest [-77.89, 167.008], Nord Est [-77.89, 167.008]

Couverture taxonomique

Bacteria and Archaea (16S ssu rRNA gene)

Domain  Bacteria (bacteria),  Archaea (Archaea)

Couverture temporelle

Date de début 2012-12-01

Données sur le projet

Pas de description disponible

Titre The Whillans Ice Stream Subglacial Access Research Drilling (WISSARD) project
Financement The Whillans Ice Stream Subglacial Access Research Drilling (WISSARD) project was funded by National Science Foundation grants (0838933, 0838896, 0838941, 0839142, 0839059, 0838885, 0838855, 0838763, 0839107, 0838947, 0838854, 0838764 and 1142123) from the Office of Polar Programs. Partial support was also provided by funds from NSF award 1023233, 1115245, the American Association of University Women Dissertation Fellowship, the NSF’s Graduate Research Fellowship Program (1247192; A.M.A.), the Chilean Fulbright-CONICYT Scholarship (P.S), the Italian National Antarctic Program, and fellowships from the NSF’s IGERT Program (0654336) and the Montana Space Grant Consortium.

Les personnes impliquées dans le projet:

John Priscu

Méthodes d'échantillonnage

A discrete water sample for nucleic acid extraction was collected in situ at 850 m with a Large Volume Water Transfer System (WTS-LV; McLane Research Laboratories, East Falmouth, Massachusetts). This system was fitted with a stacked 143 mm diameter filter housing that allowed the sample to be size fractionated into 10 um, 3 um, and 0.2 um classes (Supor Membrane filters were used; Pall). This system allowed us to concentrate 295 L of seawater on 142 mm filters during a 4 h in situ deployment. An additional discrete water sample (300 mL) from 30 m was obtained for nucleic acid extraction from a 10 L Niskin bottle sample that was fil- tered through a 47 mm 0.2 um Supor membrane filter (Pall). Filters from the WTS-LV were preserved as described previ- ously (Christner et al. 2014) while the entire 47 mm filter from the 30 m sample was placed in a 7 mL cryovial and preserved with 7 mL of a solution of 40 mM EDTA pH 8.0, 50 mM Tris pH 8.3, and 0.73 M Sucrose to prevent cell lysis during storage.

Etendue de l'étude After drilling through the Ice sheet (56m), water samples were taken from the marine cavity beneath the McMurdo Ice Shelf, Antarctica

Description des étapes de la méthode:

  1. Nucleic acids were extracted from the 10 um, 3 um, and 0.2 um filters from 850 m and the 0.2 um filter from 30 m using a MO BIO PowerWater DNA Isolation Kit according to the manufactures instructions. The V4 hypervariable region of the small subunit (SSU) rRNA gene was amplified using the primers 515F and 806R (Caporaso et al. 2012) and sequenced on an Illumina MiSeq as described by Christner et al. (2014).

Citations bibliographiques

  1. Vick-Majors T. J., Achberger A., Santibáñez P., Dore J. E., Hodson T., Michaud A. B., et al. (2016). Biogeochemistry and microbial diversity in the marine cavity beneath the McMurdo Ice Shelf, Antarctica. Limnol. Oceanogr. 61 572–586. 10.1002/lno.10234  doi: 10.1002/lno.10234

Métadonnées additionnelles

Identifiants alternatifs 4200a83c-9ff7-43e0-ae09-ef1211847673
https://ipt.biodiversity.aq/resource?r=microbes_sub_ice_seawater_antarctica