Microorganisms (Bacteria and Archaea) in the marine cavity beneath the McMurdo Ice Shelf, Antarctica

最新版本 由 SCAR - Microbial Antarctic Resource System 發佈於 Mar 19, 2019 SCAR - Microbial Antarctic Resource System

Amplicon sequencing dataset (Illumina MiSeq) of Bacteria and Archaea (16S) in the marine cavity beneath the McMurdo Ice Shelf, Antarctica

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版本

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如何引用

研究者應依照以下指示引用此資源。:

Vick-Majors T, Achberger A, Santibanez P, Dore J, Hodson T, Michaud A, Christner B, Mikucki J, Skidmore M, Powell R, Adkins P, Brabante C, Mitchell A, Scherer R, Priscu J (2019): Microorganisms (Bacteria and Archaea) in the marine cavity beneath the McMurdo Ice Shelf, Antarctica. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbes_sub_ice_seawater_antarctica&v=1.1

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The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

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關鍵字

Metadata

聯絡資訊

資源建立者:

Trista Vick-Majors
Montana State University Bozeman US
Amanda Achberger
Louisiana State University Baton Rouge US
Pamela Santibanez
Montana State University Bozeman US
John Dore
Montana State University Bozeman US
Timothy Hodson
Northern Illinois University DeKalb US
Alexander Michaud
Montana State University Bozeman US
Brent Christner
Louisiana State University Baton Rouge US
Jill Mikucki
Middlebury College Middlebury US
Mark Skidmore
Montana State University Bozeman US
Ross Powell
Northern Illinois University DeKalb US
Peyton Adkins
Louisiana State University Baton Rouge US
Carlo Brabante
University of Venice Venice IT
Andrew Mitchell
Aberystwyth University Aberystwyth GB
Reed Scherer
Northern Illinois University DeKalb US
John Priscu
Montana State University Bozeman US

可回覆此資源相關問題者:

John Priscu
Montana State University Bozeman US

元數據填寫者:

Maxime Sweetlove
Research assistent
Royal Belgian Institute of Natural Science Rue Vautier 29 1000 Brussels

與此資源的相關者:

使用者

地理涵蓋範圍

the marine cavity beneath the McMurdo Ice Shelf, Antarctica

界定座標範圍 緯度南界 經度西界 [-77.89, 167.008], 緯度北界 經度東界 [-77.89, 167.008]

分類群涵蓋範圍

Bacteria and Archaea (16S ssu rRNA gene)

Domain  Bacteria (bacteria),  Archaea (Archaea)

時間涵蓋範圍

起始日期 2012-12-01

計畫資料

無相關描述

計畫名稱 The Whillans Ice Stream Subglacial Access Research Drilling (WISSARD) project
經費來源 The Whillans Ice Stream Subglacial Access Research Drilling (WISSARD) project was funded by National Science Foundation grants (0838933, 0838896, 0838941, 0839142, 0839059, 0838885, 0838855, 0838763, 0839107, 0838947, 0838854, 0838764 and 1142123) from the Office of Polar Programs. Partial support was also provided by funds from NSF award 1023233, 1115245, the American Association of University Women Dissertation Fellowship, the NSF’s Graduate Research Fellowship Program (1247192; A.M.A.), the Chilean Fulbright-CONICYT Scholarship (P.S), the Italian National Antarctic Program, and fellowships from the NSF’s IGERT Program (0654336) and the Montana Space Grant Consortium.

The personnel involved in the project:

John Priscu

取樣方法

A discrete water sample for nucleic acid extraction was collected in situ at 850 m with a Large Volume Water Transfer System (WTS-LV; McLane Research Laboratories, East Falmouth, Massachusetts). This system was fitted with a stacked 143 mm diameter filter housing that allowed the sample to be size fractionated into 10 um, 3 um, and 0.2 um classes (Supor Membrane filters were used; Pall). This system allowed us to concentrate 295 L of seawater on 142 mm filters during a 4 h in situ deployment. An additional discrete water sample (300 mL) from 30 m was obtained for nucleic acid extraction from a 10 L Niskin bottle sample that was fil- tered through a 47 mm 0.2 um Supor membrane filter (Pall). Filters from the WTS-LV were preserved as described previ- ously (Christner et al. 2014) while the entire 47 mm filter from the 30 m sample was placed in a 7 mL cryovial and preserved with 7 mL of a solution of 40 mM EDTA pH 8.0, 50 mM Tris pH 8.3, and 0.73 M Sucrose to prevent cell lysis during storage.

研究範圍 After drilling through the Ice sheet (56m), water samples were taken from the marine cavity beneath the McMurdo Ice Shelf, Antarctica

方法步驟描述:

  1. Nucleic acids were extracted from the 10 um, 3 um, and 0.2 um filters from 850 m and the 0.2 um filter from 30 m using a MO BIO PowerWater DNA Isolation Kit according to the manufactures instructions. The V4 hypervariable region of the small subunit (SSU) rRNA gene was amplified using the primers 515F and 806R (Caporaso et al. 2012) and sequenced on an Illumina MiSeq as described by Christner et al. (2014).

引用文獻

  1. Vick-Majors T. J., Achberger A., Santibáñez P., Dore J. E., Hodson T., Michaud A. B., et al. (2016). Biogeochemistry and microbial diversity in the marine cavity beneath the McMurdo Ice Shelf, Antarctica. Limnol. Oceanogr. 61 572–586. 10.1002/lno.10234  doi: 10.1002/lno.10234

額外的元數據

替代的識別碼 4200a83c-9ff7-43e0-ae09-ef1211847673
https://ipt.biodiversity.aq/resource?r=microbes_sub_ice_seawater_antarctica