microbial Eukaryotes in lakes along an Argentinian-Antarctic geographical gradient

Última versión Publicado por SCAR - Microbial Antarctic Resource System en Mar 20, 2019 SCAR - Microbial Antarctic Resource System

Amplicon sequencing dataset (Illumina HiSeq) of Eukaryotes (18S) in lakes along a latitudinal gradient from Southern Argentina to maritime Antarctica

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¿Cómo referenciar?

Los usuarios deben citar este trabajo de la siguiente manera:

Schiaffino R, Lara E, Fernandez L, Balagué V, Singer D, Seppey C, Massana R, Izaguirre I (2019): microbial Eukaryotes in lakes along an Argentinian-Antarctic geographical gradient. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbial_eukaryotes_in_antarctic_and_argentinian_lakes&v=1.0

Derechos

Los usuarios deben respetar los siguientes derechos de uso:

El publicador y propietario de los derechos de este trabajo es SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

Registro GBIF

Este recurso ha sido registrado en GBIF con el siguiente UUID: bc44c279-bfe6-4827-80b4-06dcb9aa7908.  SCAR - Microbial Antarctic Resource System publica este recurso, y está registrado en GBIF como un publicador de datos avalado por Scientific Committee on Antarctic Research.

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Metadata

Contactos

¿Quién creó el recurso?:

Romina Schiaffino
Centro de Investigaciones y transferencia del Noroeste de la Provincia de Buenos Aires Junin AR
Enrique Lara
University of Neuchâtel Neuchâtel CH
Leonardo Fernandez
University of Neuchâtel Neuchâtel CH
Vanessa Balagué
Institut de Ciències del Mar Barcelona ES
David Singer
University of Neuchâtel Neuchâtel CH
Christophe Seppey
University of Neuchâtel Neuchâtel CH
Ramon Massana
Institut de Ciències del Mar Barcelona ES
Irina Izaguirre
Universidad de Buenos Aires Buenos Aires AR

¿Quién puede resolver dudas acerca del recurso?:

Romina Schiaffino
Centro de Investigaciones y transferencia del Noroeste de la Provincia de Buenos Aires Junin AR

¿Quién documentó los metadatos?:

Maxime Sweetlove
Research assistent
Royal Belgian Institute of Natural Sciences Rue Vautier 29 1000 Brussels BE

¿Quién más está asociado con el recurso?:

Usuario

Cobertura Geográfica

Latitudinal gradient from Patagonia and Terra del Fuego (Argentina) to the Antarctic Peninsula

Coordenadas límite Latitud Mínima Longitud Mínima [-63.4, -71.51], Latitud Máxima Longitud Máxima [-45.55, -57]

Cobertura Taxonómica

microbial eukaryotes were profiled by targeting the 18S ssu rRNA gene (v9 region)

Dominio  Eukaryota (Eukaryotes)

Cobertura Temporal

Fecha Inicial / Fecha Final 2004-01-15 / 2008-10-20

Datos del Proyecto

No hay descripción disponible

Título microbial Eukaryotes in lakes along an Argentinian-Antarctic geographical gradient
Fuentes de Financiación This work was supported by a grant from the Argentinean Funds for Technical and Scientific Investigation (FONCYT, PICT 32732 and PICT 2013‐0794), the Spanish Project MIXANTAR (REN 2002‐11396‐E/ANT), the Swiss NSF project 310003A_143960 and the Program ‘Luis Santaló’ of the National Research Council of Spain and the National Council of Scientific and Technical Research of Argentina (CSIC‐CONICET, PROBA 2007AR0018).

Personas asociadas al proyecto:

Romina Schiaffino

Métodos de Muestreo

Integrated samples were collected within the euphotic zone from the surface down to 5 m in deep lakes and from about 30 cm below the surface in shallow lakes.

Área de Estudio Samples were collected once in 14 freshwater bodies (ranging from oligotrophic to eutrophic) from Chubut Province, Argentinean Patagonia, to Hope Bay, Antarctic Peninsula (45º22′ to 63º24′ S of latitude) (Fig. 1). In Antarctic lakes, samples were taken during the austral summer 2004, whereas in Patagonian lakes, samples were collected in late spring 2007 and 2008.

Descripción de la metodología paso a paso:

  1. Water samples were pre‐filtered in situ through a 50 µm net to remove zooplankton, then filtered with a vacuum pump first through a 20 µm pore‐size polycarbonate filter and then through a 3 µm and 0.2 µm pore‐size polycarbonate filters (diameter 47 mm; Millipore, Cork, Ireland). The filters were placed in cryovials with 1.8 ml of lysis buffer (40 mM EDTA, 50 mM Tris‐HCl, 0.75 M sucrose) and stored at −80°C until DNA extraction. The 0.2–3 µm size fraction was used for this study. The procedures followed for DNA extraction (phenol/chloroform extraction) and touchdown polymerase chain reaction (PCR) amplifications were previously described in detail (Unrein et al., 2005).
  2. We amplified extracted DNA using primers specific to the V9 variable region of the 18S rRNA gene using the protocol as in Amaral‐Zettler et al. (2009), and adapted after Lara et al. (2015). Sequencing was performed by the company Fasteris (Geneva, Switzerland) using Illumina HiSeq 2500 technology; paired end reads were around 200 bp in length.

Referencias Bibliográficas

  1. Schiaffino, M. R., Lara, E., Fernández, L. D., Balagué, V., Singer, D., Seppey, C. C., ... & Izaguirre, I. (2016). Microbial eukaryote communities exhibit robust biogeographical patterns along a gradient of Patagonian and Antarctic lakes. Environmental microbiology, 18(12), 5249-5264. https://doi.org/10.1111/1462-2920.13566