Microbiome (Archaea, Bacteria and Eukaryota) of lake Fryxell and lake Bonney (McMurdo Dry Valleys, Antarctica)

Versão mais recente published by SCAR - Microbial Antarctic Resource System on mar 19, 2019 SCAR - Microbial Antarctic Resource System
Publication date:
19 de Março de 2019
Licença:
CC-BY 4.0

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Descrição

Amplicon sequencing dataset (454 pyrosequencing) of Archaea (16S), Bacteria (16S) and Eukaryote (18S) in the water column of lake Fryxell and Lake Bonney, in the McMurdo Dry valleys (Antarctica).

Versões

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Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Vick-Majors T, Priscu J, Amaral-Zettler L (2019): Microbiome (Archaea, Bacteria and Eukaryota) of lake Fryxell and lake Bonney (McMurdo Dry Valleys, Antarctica). v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbiome_mc_murdo_dry_valley_lakes_antarctica&v=1.2

Direitos

Pesquisadores devem respeitar a seguinte declaração de direitos:

O editor e o detentor dos direitos deste trabalho é SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 4b44031f-2271-48a8-b3d9-43b90768e071.  SCAR - Microbial Antarctic Resource System publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por Scientific Committee on Antarctic Research.

Palavras-chave

Metadata

Contatos

Trista Vick-Majors
  • Originador
  • Ponto De Contato
Montana State University
Bozeman
US
John Priscu
  • Originador
Montana State University
Bozeman
US
Linda Amaral-Zettler
  • Originador
he Josephine Bay Paul Center for Comparative Molecular Biology and Evolution
Woods Hole
US
Maxime Sweetlove
  • Provedor Dos Metadados
Research assistent
Royal Belgian Institute of Natural Sciences
Rue Vautier 29
1000 Brussels
BE

Cobertura Geográfica

Lake Fryxell and lake Bonney, McMurdo Dry Valleys, Antarctica

Coordenadas delimitadoras Sul Oeste [-77,72, 162,297], Norte Leste [-77,72, 163,146]

Cobertura Taxonômica

Archaea were profiled by targeting the v6 region of the 16S ssu rRNA gene

Domínio Archaea (Archaea)

Bacteria were profiled by targeting the v6 region of the 16S ssu rRNA gene

Domínio Bacteria (Bacteria)

Eukaryota were profiled by targeting the v9 region of the 18S ssu rRNA gene

Domínio Eukaryota (Eukaryotes)

Cobertura Temporal

Data Inicial / Data final 2007-11-22 / 2008-03-25

Dados Sobre o Projeto

Nenhuma descrição disponível

Título MIRADA-LTERS
Financiamento Funding was provided by NSF DEB-0717390 (MIRADA-LTERS) and OPP-1115254, OPP-0838953, OPP-1027284 and OPP- 0839075. The Montana Space Grant Consortium provided additional funding.

O pessoal envolvido no projeto:

Trista Vick-Majors

Métodos de Amostragem

Duplicate samples were collected at depths of 6 m (bacterial and primary production maximum) and 9 m (chemocline; chlorophyll-a maximum) in lake Fryxell (2 November 2007 and 25 March 2008) and 13 m (chemocline; bacterial production, chlorophyll-a, primary production maximum) and 18 m (hypersaline, bottom of trophogenic zone) in lake Bonney (west Lobe; 30 November 2007 and 12 March 2008). Samples for DNA extraction were filtered onto 0.2-μM Sterivex filters (Millipore, Billerica, MA, USA) and stored with 2.0 ml of Puregene lysis buffer at −20 °C until further processing.

Área de Estudo Sampling occurred during the Austral summer and autumn in lake Fryxell and lake Bonney, Antarctica

Descrição dos passos do método:

  1. DNA extraction protocols can be found at http://amarallab.mbl.edu.
  2. The V6 hypervariable regions of Archaa and bacteria were amplified, while for Eukaryota, amplification of the V9 hypervariable region followed established protocols (Amaral-Zettler et al., 2009). We multiplex-sequenced the resulting amplicons using bar-coded primers (Huber et al., 2007; Amaral-Zettler et al., 2009) on a 454 Genome Sequencer FLX (Roche, Switzerland) using the manufacturer’s recommended protocol.

Citações bibliográficas

  1. Vick-Majors, T. J., Priscu, J. C., & Amaral-Zettler, L. A. (2014). Modular community structure suggests metabolic plasticity during the transition to polar night in ice-covered Antarctic lakes. The ISME journal, 8(4), 778.
  2. Xu, Y., Vick-Majors, T., Morgan-Kiss, R., Priscu, J. C., & Amaral-Zettler, L. (2014). Ciliate diversity, community structure, and novel taxa in lakes of the McMurdo Dry Valleys, Antarctica. The Biological Bulletin, 227(2), 175-190.

Metadados Adicionais

Identificadores alternativos 4b44031f-2271-48a8-b3d9-43b90768e071
https://ipt.biodiversity.aq/resource?r=microbiome_mc_murdo_dry_valley_lakes_antarctica