Bacterioplankton in surface waters off the northern tip of the Antarctic Peninsula

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Последняя версия опубликовано SCAR - Microbial Antarctic Resource System июн 10, 2021 SCAR - Microbial Antarctic Resource System
Дата публикации:
10 июня 2021 г.
Опубликовано:
SCAR - Microbial Antarctic Resource System
Лицензия:
CC-BY 4.0

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Описание

Amplicon sequencing dataset (454 LS) targeting planktonic Bacteria (16S ssu rRNA) in surface layer sea water samples (n=18) from the Northern tip of the Antarctic Peninsula.

Версии

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Как оформить ссылку

Исследователи должны дать ссылку на эту работу следующим образом:

Cao S, He J, Zhang F, Lin L, Gao Y, Zhou Q (2021): Bacterioplankton in surface waters off the northern tip of the Antarctic Peninsula. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=bacterioplankton_from_northern_tip_antarctic_peninsula&v=1.0

Права

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Публикующей организацией и владельцем прав на данную работу является SCAR - Microbial Antarctic Resource System. Эта работа находится под лицензией Creative Commons Attribution (CC-BY 4.0).

Регистрация в GBIF

Этот ресурс был зарегистрирован в GBIF, ему был присвоен следующий UUID: 6acade15-6d79-4dad-824e-a70b73db623c.  SCAR - Microbial Antarctic Resource System отвечает за публикацию этого ресурса, и зарегистрирован в GBIF как издатель данных при оподдержке Scientific Committee on Antarctic Research.

Ключевые слова

Metadata

Контакты

Shunan Cao
  • Originator
The Key Laboratory for Polar Science, State Ocean Administration, Polar Research Institute of China
Shanghai
CN
Jianfeng He
  • Originator ●
  • User ●
  • Point Of Contact
The Key Laboratory for Polar Science, State Ocean Administration, Polar Research Institute of China
Shanghai
CN
Fang Zhang
  • Originator
The Key Laboratory for Polar Science, State Ocean Administration, Polar Research Institute of China
Shanghai
CN
Ling Lin
  • Originator
The Key Laboratory for Polar Science, State Ocean Administration, Polar Research Institute of China
Shanghai
CN
Yuan Gao
  • Originator
The Key Laboratory for Polar Science, State Ocean Administration, Polar Research Institute of China
Shanghai
CN
Qiming Zhou
  • Originator
School of Life Science and Technology, Harbin Institute of Technology
Harbin
CN
Maxime Sweetlove
  • Metadata Provider
Royal Belgian Institute of Natural Sciences
Brussels
BE

Географический охват

Northern tip of the Antarctic Peninsula

Ограничивающие координаты Юг Запад [-65,613, -66,441], Север Восток [-64,815, -62,593]

Временной охват

Дата начала / Дата окончания 2011-12-30 / 2012-01-29

Данные проекта

Описание отсутсвует

Название CHINARE-2011–2015
Финансирование This work was funded by the Chinese Polar Environment Comprehensive Investigation & Assessment Programs (CHINARE-2011–2015), an international cooperation programme of the Chinese National Arctic and Antarctic Research Expedition (IC201514) and the National Natural Science Foundation of China (grant nos. 41206189 and 41476168).
Описание района исследования Seawater from the northern tip of the Antarctic Peninsula.

Исполнители проекта:

Jianfeng He

Методы сбора

Thirteen samples were taken at 25 m depth (the layer of maximum oxygen), three samples were taken at 2 m and two samples were taken at 50 m depth. An SBE 911 plus CTD instrument combined with an SBE 32 Carousel water sampler (both by Sea-Bird Electronics) equipped with 24 Niskin bottles was used to collect seawater and measure physical parameters (temperature and salinity). +- 2 L of seawater was pre-filtered through 3 μm pore size polycarbonate membranes (Whatman), and then filtered with a vacuum pump through polycarbonate membranes (47 mm diameter, 0.2-μm pore size, Whatman). Samples were subsequently frozen at −80°C.

Охват исследования Samples (n=18) were collected in the waters by the northern tip of the Antarctic Peninsula, including the northern area of the Bransfield Strait, the Powell Basin and the South Orkney tableland area during the 28th Chinese National Antarctic Research Expedition, from December 2011 to January 2012.
Контроль качества The PCR products were purified using AxyPrepTM DNA Purification Kit (Axygen®) and quantified using a Qubit® 2.0 Fluorometer (Life Technologies).

Описание этапа методики:

  1. DNA was extracted using a modified cetyltrimethylammonium bromide method and examined by agarose gel electrophoresis.
  2. The V1–V3 region of the 16S rRNA gene of Bacteria was amplified using the universal primer pair F8 (5′-CCTATCCCCTGTGT- GCCTTGGCAGTCTCAG-AGAGTTTGATCCTGGCTCAG-3′) and R533 (5′-CCATCTCATCCCTGC- GTGTCTCCGACTCAG-NNNNNNNN-TTACCGCGGCTGCT- GGCAC-3′; NNNNNNNN being the place of the sample-specific barcode). PCR was performed using 5–10 ng genomic DNA in a final volume of 50 μL. The PCR procedure was as follows: initial denaturation at 95°C for 2 min; 25 cycles at 95°C for 30 s, 56.4°C for 1 min and 72°C for 30 s; and a final extension at 72°C for 5 min.
  3. 454 pyrosequencing was performed on an FLX Titanium Genome Sequencer (454/Roche Life Sciences).

Библиографические ссылки

  1. Cao, S., He, J., Zhang, F., Lin, L., Gao, Y., & Zhou, Q. (2019). Diversity and community structure of bacterioplankton in surface waters off the northern tip of the Antarctic Peninsula. Polar Research.

Дополнительные метаданные