cyanobacterial mats of from the Byers Peninsula, Antarctica

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Versão mais recente published by SCAR - Microbial Antarctic Resource System on mar 19, 2019 SCAR - Microbial Antarctic Resource System
Publication date:
19 de Março de 2019
Licença:
CC-BY 4.0

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Descrição

Cyanobacterial 16S rRNA gene sequences from cyanobacterial mats of Antarctic (Byers Peninsula) origin obtained by clone library

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Direitos

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O editor e o detentor dos direitos deste trabalho é SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

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Metadata

Contatos

Julia Kleinteich
  • Originador ●
  • Ponto De Contato
Researcher
University of Liège
Liège
Lieège
BE
Julien Cigar
  • Provedor Dos Metadados
Daniel R Dietrich
  • Ponto De Contato
Researcher
University of Konstanz
Konztanz
DE

Cobertura Geográfica

Byers Peninsula, Livingston Island, Antarctica

Coordenadas delimitadoras Sul Oeste [-62,7, -61,5], Norte Leste [-62,5, -61]

Cobertura Taxonômica

All data provided are OTUs of the 16S rRNA gene which were identified via a comparison to online databases to family or genus level. For most OTUs identification to species level was not possible

Filo Cyanobacteria (Cyanobacteria)

Cobertura Temporal

Data Inicial / Data final 2009-02-01 / 2009-02-28

Dados Sobre o Projeto

Nenhuma descrição disponível

Título DI698/18-1 Dietrich
Financiamento Deutsche Forschungsgesellschaft DFG

O pessoal envolvido no projeto:

Daniel R Dietrich
  • Pesquisador Principal

Métodos de Amostragem

Cyanobacterial samples were collected from five different sampling sites in the Antarctic Specially Protected Area (ASPA) No. 126 of Byers Peninsula, Livingston Island (62° 34' 35" to 62° 4' 35" S and 60° 54' 14" to 61° 13' 07" W), South Shetland Islands, Antarctic Peninsula, during an expedition in February 2009. Microbial mats were probed using a sterile spatula, sealed in sterile plastic bags or tubes and stored frozen (-20 °C) for DNA extraction.

Área de Estudo Samples were collected on Byers Peninsula (ASPA 126) in February 2009. This area is a summer snow- and ice-free area. Various microbial mats on wet soil, meltwater and seepages were sampled.
Controle de Qualidade Data were published in a peer-reviewed journal DOI: 10.1038/NCLIMATE1418

Descrição dos passos do método:

  1. After collection the samples were stored at -20°C until further processing. DNA was extracted from the samples in three replicates and combined. 16S rRNA genes were amplified using cyanobacteria specific primers (Saker et al. 2005). PCR Products were cloned using the TOPO TA Cloning Kit following the standard protocol. Two to three clones of each individual restriction fragment length polymorphism pattern were sequenced at GATC Biotech. All sequences were deposited with the GenBank database. Saker, M. L., Jungblut, A. D., Neilan, B. A., Rawn, D. F. K. & Vasconcelos, V. M. Detection of microcystin synthetase genes in health food supplements containing the freshwater cyanobacterium Aphanizomenon flos-aquae. Toxicon 46, 555?562 (2005).

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