Global biogeography of desert cyanobacteria

最新版本 published by SCAR - Microbial Antarctic Resource System on 三月 19, 2019 SCAR - Microbial Antarctic Resource System
發布日期:
2019年3月19日
授權條款:
CC-BY 4.0

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說明

Amplicon sequencing dataset (454 pyrosequencing) of Bacteria (16S ssu rRNA) and Cyanobacteria (nifH) in cold desert quartz rocks

版本

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如何引用

研究者應依照以下指示引用此資源。:

Lacap-bugler D, Lee K, Archer S, Gillman L, Lau M, Leuzinger S, Lee C, Maki T, McKay C, Perrott J, de los Rios-Murillo A, Warren-Rhodes K, Hopkins D, Pointing S (2018): Global biogeography of desert cyanobacteria. v1.4. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=desert_cyanobacteria&v=1.4

權利

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此資料的發布者及權利單位為 SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF 註冊

此資源已向GBIF註冊,並指定以下之GBIF UUID: 914f39c7-7bb2-4e77-a4ae-36147aa07daf。  SCAR - Microbial Antarctic Resource System 發佈此資源,並經由Scientific Committee on Antarctic Research同意向GBIF註冊成為資料發佈者。

關鍵字

Metadata

聯絡資訊

Donnabella Lacap-bugler
  • 出處
  • 連絡人
Auckland University of Technology
Auckland
NZ
Kevin Lee
  • 出處
Auckland University of Technology
Auckland
NZ
Stephen Archer
  • 出處
Auckland University of Technology
Auckland
NZ
Len Gillman
  • 出處
Auckland University of Technology
Auckland
NZ
Maggie Lau
  • 出處
Princeton University
Princeton
US
Sebastian Leuzinger
  • 出處
Auckland University of Technology
Auckland
NZ
Charles Lee
  • 出處
University of Waikato
Hamilton
NZ
Teruya Maki
  • 出處
Kanazawa University
Kanazawa
JP
Christoffer McKay
  • 出處
National Aeronautics and Space Administration Ames Research Center
Moffett Field
US
John Perrott
  • 出處
Auckland University of Technology
Auckland
NZ
Asunción de los Rios-Murillo
  • 出處
Museo Nacional de Ciencias Naturales
Madrid
ES
Kimberley Warren-Rhodes
  • 出處
Kanazawa University
Kanazawa
JP
David Hopkins
  • 出處
The Royal Agricultural University
Cirencester
GB
Stephen Pointing
  • 出處
Auckland University of Technology
Auckland
NZ
Maxime Sweetlove
  • 元數據提供者
Research assistent
Royal Belgian Institute for Natural Sciences
Rue Vautier 29
1000 Brussels

地理涵蓋範圍

Tibetan Plateau, China; Taklimakan Desert, China; Devon Island (Arctic), Canada; McMurdo Dry Valleys, Antarctica

界定座標範圍 緯度南界 經度西界 [-77.41, 88.616], 緯度北界 經度東界 [42.715, 161.193]

分類群涵蓋範圍

Bacteria (16S ssu rRNA) and Cyanobacteria (nifH)

Domain Bacteria (Bacteria)
Phylum Cyanobacteria (Cyanobacteria)

計畫資料

無相關描述

計畫名稱 Global biogeography of desert cyanobacteria
經費來源 The research was funded by the NASA Astrobiology Science and Technology for Exploring Planets (ASTEP) program and the Institute for Applied Ecology New Zealand (www.aenz.aut.ac.nz).

參與計畫的人員:

Donnabella Lacab-bugler

取樣方法

Colonized quartz stones were retrieved by hand (using isopropyl alcohol surface sterilized latex gloves) and loose soil particles gently removed with a sterile (autoclaved) paintbrush. Samples were then stored in sterile Whirlpak bags (Nasco) at -20°C in the field and in transit, and subsequently stored frozen at -80°C in the laboratory until processed.

研究範圍 Hypolithic communities were recovered from quartz substrate in desert pavement on the Tibetan Plateau (China), the Taklimakan Desert (China), Devon Island (Canada) and the McMurdo Dry Valleys (Antarctica).

方法步驟描述:

  1. Amplification of 16S rRNA genes was achieved using primer pair 341F and 907R with PCR conditions involving an initial denaturation time of 5min; 30 cycles at 95°C for 1 min, 55°C for 1 min, 72°C for 1 min, and a final extension at 72°C for 10 min. Positive and negative controls were run for every PCR. For each amplicon library purification was carried out with Agencourt AMPure XP Bead (Beckman Coulter, CA, USA) according to manufacturer’s instructions. The libraries were quantified with Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen Life Technologies, NY, USA) using FLUOstar OPTIMA F fluorometer (BMG Labtech GmbH, Offenburg, Germany) and library quality was assessed with the FlashGel System (Lonza Group Ltd., Basel, Switzerland).
  2. Emulsion-PCR was carried out with GS Junior Titanium emPCR Kit (Lib-L, 454 Life Sciences Corp., CT, USA) according to the emPCR Amplification Method Manual – Lib-L, Single-Prep. The sequencing reaction was carried out with the GS Junior Titanium Sequencing Kit and GS Junior Titanium PicoTiterPlate Kit (454 Life Sciences Corp.) according to the manufacturer’s instructions. The sequencing run was conducted in 200 cycles.

引用文獻

  1. Lacap-Bugler, D. C., Lee, K. K., Archer, S., Gillman, L. N., Lau, M. C., Leuzinger, S., ... & de los Rios-Murillo, A. (2017). Global diversity of desert hypolithic cyanobacteria. Frontiers in microbiology, 8, 867.

額外的詮釋資料

替代的識別碼 914f39c7-7bb2-4e77-a4ae-36147aa07daf
https://ipt.biodiversity.aq/resource?r=desert_cyanobacteria