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Lacab-bugler D, Lee K, Archer S, Gillman L, Lau M, Leuzinger S, Lee C, Maki T, McKay C, Perrott J, de los Rios-Murillo A, Warren-Rhodes K, Hopkins D, Pointing S (2018): Global biogeography of desert cyanobacteria. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. http://ipt.biodiversity.aq/resource?r=desert_cyanobacteria&v=1.0
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The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
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Metadata
聯絡資訊
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地理涵蓋範圍
Tibetan Plateau, China; Taklimakan Desert, China; Devon Island (Arctic), Canada; McMurdo Dry Valleys, Antarctica
| 界定座標範圍 | 緯度南界 經度西界 [-77.41, 88.616], 緯度北界 經度東界 [42.715, 161.193] |
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分類群涵蓋範圍
Bacteria (16S ssu rRNA) and Cyanobacteria (nifH)
| Domain | Bacteria (Bacteria) |
|---|---|
| Phylum | Cyanobacteria (Cyanobacteria) |
計畫資料
無相關描述
| 計畫名稱 | Global biogeography of desert cyanobacteria |
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| 經費來源 | The research was funded by the NASA Astrobiology Science and Technology for Exploring Planets (ASTEP) program and the Institute for Applied Ecology New Zealand (www.aenz.aut.ac.nz). |
The personnel involved in the project:
取樣方法
Colonized quartz stones were retrieved by hand (using isopropyl alcohol surface sterilized latex gloves) and loose soil particles gently removed with a sterile (autoclaved) paintbrush. Samples were then stored in sterile Whirlpak bags (Nasco) at -20°C in the field and in transit, and subsequently stored frozen at -80°C in the laboratory until processed.
| 研究範圍 | Hypolithic communities were recovered from quartz substrate in desert pavement on the Tibetan Plateau (China), the Taklimakan Desert (China), Devon Island (Canada) and the McMurdo Dry Valleys (Antarctica). |
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方法步驟描述:
- Amplification of 16S rRNA genes was achieved using primer pair 341F and 907R with PCR conditions involving an initial denaturation time of 5min; 30 cycles at 95°C for 1 min, 55°C for 1 min, 72°C for 1 min, and a final extension at 72°C for 10 min. Positive and negative controls were run for every PCR. For each amplicon library purification was carried out with Agencourt AMPure XP Bead (Beckman Coulter, CA, USA) according to manufacturer’s instructions. The libraries were quantified with Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen Life Technologies, NY, USA) using FLUOstar OPTIMA F fluorometer (BMG Labtech GmbH, Offenburg, Germany) and library quality was assessed with the FlashGel System (Lonza Group Ltd., Basel, Switzerland).
- Emulsion-PCR was carried out with GS Junior Titanium emPCR Kit (Lib-L, 454 Life Sciences Corp., CT, USA) according to the emPCR Amplification Method Manual – Lib-L, Single-Prep. The sequencing reaction was carried out with the GS Junior Titanium Sequencing Kit and GS Junior Titanium PicoTiterPlate Kit (454 Life Sciences Corp.) according to the manufacturer’s instructions. The sequencing run was conducted in 200 cycles.
引用文獻
- Lacap-Bugler, D. C., Lee, K. K., Archer, S., Gillman, L. N., Lau, M. C., Leuzinger, S., ... & de los Rios-Murillo, A. (2017). Global diversity of desert hypolithic cyanobacteria. Frontiers in microbiology, 8, 867.