Geomicrobiology of Antarctic Subglacial Environments - Subglacial Lake Whillans

Outro
Versão mais recente published by SCAR - Microbial Antarctic Resource System on mar 19, 2019 SCAR - Microbial Antarctic Resource System
Início:
http://www.wissard.org/
Publication date:
19 de março de 2019
Licença:
CC-BY 4.0

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Descrição

Liquid water has been known to occur beneath the Antarctic ice sheet for more than 40 years,but only recently have these subglacial aqueous environments been recognized as microbial ecosystems that may influence biogeochemical transformations on a global scale. Here we present the first geomicrobiological description of water and surficial sediments obtained from direct sampling of a subglacial Antarctic lake. Subglacial Lake Whillans (SLW)lies beneath approximately 800m of ice on the lower portion of the Whillans Ice Stream (WIS) in West Antarctica and is part of an extensive and evolving subglacial drainage network. The water column of SLW contained metabolically active microorganisms and was derived primarily from glacial ice melt with solute sources from lithogenic weathering and a minor seawater component. Heterotrophic and autotrophic production data together with small subunit ribosomal RNA gene sequencing and biogeochemical data indicate that SLW is a chemosynthetically driven ecosystem inhabited by a diverse assemblage of bacteria and archaea. Our results confirm that aquatic environments beneath the Antarctic ice sheet support viable microbial ecosystems, corroborating previous reports suggesting that they contain globally relevant pools of carbon and microbes that can mobilize elements from the lithosphere and influence Southern Ocean geochemical and biological systems.

Versões

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Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Christner BC, Priscu JC, Achberger A, Barbante C, Carter SP, Christianson K, Mikucki JA,Michaud AB, Mitchell A, Skidmore ML, Vick-Majors TJ, and the WISSARD Science Team. 2014. A microbial ecosystem beneath the West Antarctic Ice Sheet. Nature. 512:310-313.

Direitos

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O editor e o detentor dos direitos deste trabalho é SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 836fe3e7-98f5-4c32-b661-8df8f575d7c0.  SCAR - Microbial Antarctic Resource System publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por Scientific Committee on Antarctic Research.

Palavras-chave

Subglacial Lake Whillans; Antarctic; subglacial; geomicrobiology; microbiology; bacteria; archaea; Metadata

Contatos

John Priscu
  • Provedor Dos Metadados ●
  • Originador ●
  • Ponto De Contato ●
  • Pesquisador Principal
  • Professor
Montana State University
  • 334 Leon Johnson Hall
59717 Bozeman
US
  • 4069943250
Brent Christner
  • Pesquisador Principal
  • Associate Professor
Louisiana State University
  • 282 Life Sciences Building
70803 Baton Rouge
LA
US
  • 2255781734
Mark Skidmore
  • Pesquisador Principal
  • Associate Professor
Montana State University
  • 627 Leon Johnson Hall
59717 Bozeman
MT
US
  • 4069947251

Cobertura Geográfica

Samples were collected from Subglacial Lake Whillans, at the lower portion of the Whillans Ice Stream, West Antarctica. Samples were collected through a 800m borehole created with a clean, hot water drill system.

Coordenadas delimitadoras Sul Oeste [-85,02, -155,33], Norte Leste [-83,79, -131,77]

Cobertura Taxonômica

Planktonic microbial cells were collected on 0.2, 0.8, 3.0, and 10.0 micron filters and community structure was determined from 16S rRNA gene identities. Microbial community structure was also determined for samples from a shallow sediment core (0-40 cm).

Domínio Bacteria, Archaea

Cobertura Temporal

Data Inicial 2013-02-01

Dados Sobre o Projeto

Nenhuma descrição disponível

Título Whillans Ice Stream Subglacial Access Research and Drilling - Geomicrobiology of Subglacial Lake Whillans
Financiamento The Whillans Ice StreamSubglacial Access Research Drilling (WISSARD) project was funded by National Science Foundation grants (0838933, 0838896, 0838941, 0839142, 0839059, 0838885, 0838855, 0838763, 0839107, 0838947, 0838854, 0838764 and 1142123) from the Division of Polar Programs. Partial support was also provided by funds from NSF award 1023233 (B.C.C.), NSF award1115245(J.C.P.), the NSF’s Graduate Research FellowshipProgram(1247192; A.M.A.), the Italian National Antarctic Program (C.B.), and fellowships from the NSF’s IGERT Program(0654336) and the Montana Space Grant Consortium (A.B.M.)
Descrição da Área de Estudo Water and sediments of Subglacial Lake Whillans, located 800 meters beneath the surface of the Whillans Ice Stream, West Antarctica.
Descrição do Design A hot water drilling system was used between 23–27 January 2013 to melt through the ~801 m thick ice sheet, creating an access borehole (minimum diameter ~60 cm) for direct sampling and to conduct in situ measurements of the SLW water column and sediments. Microbial cells in the drilling water and on exposed surfaces of the hose, cables, and deployed equipment were reduced and killed through the use of four complementary technologies: (1) filtration, (2) ultraviolet irradiation, (3) pasteurization, and (4) disinfection with 3% w/v H2O2. The drilling water, derived from the overlying ice sheet, was continuously circulated through a water treatment system that removed micron and sub-micron sized particles (>0.2 μm), irradiated the drilling water with two germicidal wavelengths of ultraviolet radiation (185 nm ~40,000 µW s−1 cm−2 and 254 nm ~175,000 µW s−1 cm−2), and pasteurized the water at 90 °C to reduce the viability of persisting microbial contamination. Ports were plumbed along the system’s flow path, allowing discrete water samples to be obtained before and after each stage. The drill hose and instrument cables were deployed at a rate no greater than 1 m s−1 through a custom borehole collar that contained 12 amalgam pellet ultraviolet lamps, providing a cumulative germicidal ultraviolet dosage of at least 40,000 µW s−1 cm−2 (Arapahoe SciTech). All borehole sampling tools and instruments were spray-saturated with 3% w/v H2O2 and staged in sealed polyethylene bags until tool deployment. Single-use protective apparel (Tyvek) was worn by all personnel during borehole science operations. The efficacy of the clean access technology and procedures were tested thoroughly before use in the field.

O pessoal envolvido no projeto:

John Priscu
  • Pesquisador Principal

Métodos de Amostragem

Water samples were collected and brought to the surface in Niskin bottles. Particulate matter for DNA sequence analyses was collected on 10.0, 3.0, 0.8, and 0.2 micron filters by an in situ filtration unit. Sediment samples were collected and brought to the surface using a shallow sediment multicorer. See Christner, et al 2014 for details.

Área de Estudo See Geographic Coverage
Controle de Qualidade Paired end sequence reads were assembled and quality filtered using the Mothur phylogenetic analysis pipeline (v1.33.2). The sequences were aligned with the SILVA Incremental Aligner47 (SINA v1.2.11; database release 115). The aligned reads were checked for chimaeras using the Uchime algorithm, as implemented within Mothur, and chimaeric sequences were removed from the data. Sequences with >97% SSU rRNA gene sequence similarity were clustered into an OTU and representative sequences for each OTU were chosen for classification using the SILVA database.

Descrição dos passos do método:

  1. Descriptions can be found in Christner et al., 2014. Detailed geochemical methods can be found at http://www.geosociety.org/datarepository/2016/2016110.pdf and https://www.nature.com/articles/ngeo2992#methods and https://www.frontiersin.org/articles/10.3389/fmicb.2016.01705/full Further detailed methods for microbial community composition can be found at https://www.frontiersin.org/articles/10.3389/fmicb.2016.01457/full#h3

Citações bibliográficas

  1. Michaud AB, Skidmore ML, Mitchell AC, Vick-Majors TJ, Barbante C, Turetta C, vanGelder W, Priscu JC. 2016. Solute sources and geochemical processes in Subglacial Lake Whillans, West Antarctica. Geology. 44:347-350. https://doi.org/10.1130/G37639.1
  2. Michaud AB, Dore JE, Achberger AM, Christner BC, Mitchell AC, Skidmore ML, Vick-Majors TJ, Priscu JC. 2017. Microbial oxidation as a methane sink beneath the West Antarctic Ice Sheet. Nature Geoscience. 10:582-586. doi:10.1038/ngeo2992
  3. Achberger AM, Christner BC, Michaud AB, Priscu JC, Skidmore ML, Vick-Majors TJ, and the WISSARD Science Team. 2016. Microbial Community Structure of Subglacial Lake Whillans, West Antarctica. Frontiers in Microbiology. 7:1457. doi: 10.3389/fmicb.2016.01457
  4. Vick-Majors TJ, Mitchell AC, Achberger AM, Christner BC, Dore JE, Michaud AB, Mikucki JA, Purcell AM, Skidmore ML, Priscu JC, and The WISSARD Science Team. 2016. Physiological Ecology of Microorganisms in Subglacial Lake Whillans. Frontiers in Microbiology. 7:1705. doi: 10.3389/fmicb.2016.01705
  5. Christner, B. C., Priscu, J. C., Achberger, A. M., Barbante, C., Carter, S. P., Christianson, K., ... & Vick-Majors, T. J. (2014). A microbial ecosystem beneath the West Antarctic ice sheet. Nature, 512(7514), 310.

Metadados Adicionais

Descrição da manutenção Updated metadata to include links to detailed methods from recent publications from the WISSARD-GBASE project.
Identificadores alternativos 836fe3e7-98f5-4c32-b661-8df8f575d7c0
https://ipt.biodiversity.aq/resource?r=gbase