Inter-Valley Soil Comparative Survey of the McMurdo Dry Valleys

元數據
最新版本 published by SCAR - Microbial Antarctic Resource System on 3月 19, 2019 SCAR - Microbial Antarctic Resource System
發布日期:
2019年3月19日
授權條款:
CC-BY-NC 4.0

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說明

Recent applications of molecular genetics to edaphic microbial communities of the McMurdo Dry Valleys and elsewhere have rejected a long-held belief that Antarctic soils contain extremely limited microbial diversity. The Inter-Valley Soil Comparative Survey aims to elucidate the factors shaping these unique microbial communities and their biogeography by integrating molecular genetic approaches with biogeochemical analyses. Although the microbial communities of Dry Valley soils may be complex, there is little doubt that the ecosystem’s food web is relatively simple, and evidence suggests that physicochemical conditions may have the dominant role in shaping microbial communities. To examine this hypothesis, bacterial communities from representative soil samples collected in four geographically disparate Dry Valleys were analyzed using molecular genetic tools, including pyrosequencing of 16S rRNA gene PCR amplicons. Results show that the four communities are structurally and phylogenetically distinct, and possess significantly different levels of diversity. Strikingly, only 2 of 214 phylotypes were found in all four valleys, challenging a widespread assumption that the microbiota of the Dry Valleys is composed of a few cosmopolitan species. Analysis of soil geochemical properties indicated that salt content, alongside altitude and Cu2+, was significantly correlated with differences in microbial communities. Our results indicate that the microbial ecology of Dry Valley soils is highly localized and that physicochemical factors potentially have major roles in shaping the microbiology of ice-free areas of Antarctica. These findings hint at links between Dry Valley glacial geomorphology and microbial ecology, and raise previously unrecognized issues related to environmental management of this unique ecosystem.

版本

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如何引用

研究者應依照以下指示引用此資源。:

Lee CK, Barbier BA, Bottos EM, McDonald IR, Cary SC (2012) The Inter-Valley Soil Comparative Survey: the ecology of Dry Valley edaphic microbial communities. ISME J 6: 1046–1057. doi:10.1038/ismej.2011.170.

權利

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此資料的發布者及權利單位為 SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC 4.0) License.

GBIF 註冊

此資源已向GBIF註冊，並指定以下之GBIF UUID: cca539d1-3e43-49bb-8e1f-8c1bda934a8c。  SCAR - Microbial Antarctic Resource System 發佈此資源，並經由Scientific Committee on Antarctic Research同意向GBIF註冊成為資料發佈者。

關鍵字

Bacteria; Cyanobacteria; Fungi; Terrestrial Antarctica; McMurdo Dry Valleys; 16S rRNA; Soil; Metadata

聯絡資訊

Charles Lee
  • 作者
  • Senior Lecturer
University of Waikato
  • Private Bag 3105
3240 Hamilton
NZ
  • +64 7 838 4241
Charles Lee
  • 作者
  • Research Fellow
International Center for Terrestrial Antarctic Research
  • Private Bag 3105
3240 Hamilton
NZ
  • +64 7 838 4241
Craig Cary
  • 研究主持人
  • Professor
International Center for Terrestrial Antarctic Research
  • Private Bag 3105
3240 Hamilton
NZ
  • +64 7 838 4593

地理涵蓋範圍

The McMurdo Dry Valleys

界定座標範圍 緯度南界 經度西界 [-78.091, 160.764], 緯度北界 經度東界 [-76.912, 163.809]

分類群涵蓋範圍

Molecular surveys of bacterial (16S rRNA) and fungal (ITS) marker genes

Domain Bacteria
Kingdom Fungi

時間涵蓋範圍

起始日期 / 結束日期 2006-12-01 / 2006-12-31
起始日期 / 結束日期 2008-01-01 / 2008-01-31
起始日期 / 結束日期 2010-11-01 / 2010-11-30

計畫資料

無相關描述

計畫名稱 Inter-Valley Soil Comparative Survey of the McMurdo Dry Valleys
經費來源 Funding was provided by the New Zealand Foundation for Research, Science and Technology (FRST) (UOWX0715) and the New Zealand Marsden Fund (UOW1003). Logistics support was provided by Antarctica New Zealand.
研究區域描述 Soil was collected at six different sites in the McMurdo Dry Valleys: Alatna Valley, Battleship Promontory, Upper Wright Valley, Beacon Valley, University Valley, and Miers Valley.

參與計畫的人員:

Craig Cary
  • 研究主持人

取樣方法

Sampling sites were all located on a south facing, 0–20°slope. An intersection was made by two 50 m transects, with the intersection in the middle being the central sampling point (X or C). Four sampling points around the central point were marked (A–D with A being the southernmost point and the remaining points in an anti-clockwise order, or N, E, S, W). Five scoops of the top 2 cm of soil were collected and homogenized at each identified (1 m2) sampling point after pavement pebbles were removed. Samples were stored in sterile Whirl-Pak (Nasco International, Fort Atkinson, WI, USA) at −20 °C until returned to New Zealand, where they were stored at −80 °C until analysis.

研究範圍 See Geographic Coverage
品質控管 454 pyrosquencing flowgrams were denoised using AmpliconNoise, including a SeqNoise step to remove PCR errors and a Perseus step to remove PCR chimeras. See Lee et al 2012 and Dreesens et al 2014 for details.

方法步驟描述:

  1. Two MICROBIAL_SEQUENCE_SET description files describing X data sets was uploaded to the IPT. X MIMARKS data files were uploaded to the IPT.

收藏資料

蒐藏名稱 Soil Samples from McMurdo Dry Valleys, Antarctica
蒐藏編號 N/A
上層採集品識別碼 N/A
標本保存方法 Deep frozen

引用文獻

  1. Dreesens L, Lee CK, Cary SC (2014) The Distribution and Identity of Edaphic Fungi in the McMurdo Dry Valleys. Biology 3: 466–483. doi:10.3390/biology3030466. 10.3390/biology3030466

額外的詮釋資料

替代的識別碼 cca539d1-3e43-49bb-8e1f-8c1bda934a8c
https://ipt.biodiversity.aq/resource?r=ictar_ivscs