元數據

Bacteria and Archaea biodiversity in Arctic and Subarctic terrestrial ecosystems in Alaska

最新版本 由 SCAR - Microbial Antarctic Resource System 發佈於 2022年4月15日 SCAR - Microbial Antarctic Resource System
發布日期:
2022年4月15日
授權條款:
CC-BY 4.0

下載最新版本資源元數據的EML或RTF文字檔。

元數據EML檔 下載 在 English 中 (18 KB)
元數據RTF文字檔 下載 在 English 中 (13 KB)

說明

Methane emissions from aquatic and terrestrial ecosystems play a crucial role in global warming, which is particularly affecting high-latitude ecosystems. As major contributors to methane emissions in natural environments, the microbial communities involved in methane production and oxidation deserve a special attention. Microbial diversity and activity are expected to be strongly affected by the already observed (and further predicted) temperature increase in high-latitude ecosystems, eventually resulting in disrupted feedback methane emissions. The METHANOBASE project has been designed to investigate the intricate relations between microbial diversity and methane emissions in Arctic, Subarctic and Subantarctic ecosystems, under natural (baseline) conditions and in response to simulated temperature increments. We report here a small subunit ribosomal RNA (16S rRNA) analysis of lake, peatland and mineral soil ecosystems.

版本

以下的表格只顯示可公開存取資源的已發布版本。

如何引用

研究者應依照以下指示引用此資源。:

Barret M, Thalasso F, Gandois L, Martinez Cruz K, Sepulveda Jaureguy A, Lavergne C, Teisserenc R, Aguilar P, Gerardo-Nieto O, Etchebehere C, Martins B, Fochesatto J, Tananaev N, Svenning M, Seppey C, Tveit A, Chamy R, Soledad Astorga-España M, Mansilla A, Van de Putte A, Sweetlove M, Murray A, Cabrol L (2022): Bacteria and Archaea biodiversity in Arctic and Subarctic terrestrial ecosystems in Alaska. v1.4. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=methanobasealaska&v=1.4

權利

研究者應尊重以下權利聲明。:

此資料的發布者及權利單位為 SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF 註冊

此資源已向GBIF註冊,並指定以下之GBIF UUID: 0ea51b6e-d02f-495e-a29b-73783e4060c0。  SCAR - Microbial Antarctic Resource System 發佈此資源,並經由Scientific Committee on Antarctic Research同意向GBIF註冊成為資料發佈者。

關鍵字

metadata; methane; greenhouse gas; bacteria; archaea; procaryote; peatland; wetland; soil; lake; sediment; metabarcoding; 16S rRNA; MiSeq; Metadata

聯絡資訊

資源建立者:
-

Maialen Barret
Associate professor
Laboratoire Ecologie Fonctionnelle et Environnement (UMR5245, Université de Toulouse, France)
Toulouse
FR
Frederic Thalasso
Cinvestav
Mexico City
MX
Laure Gandois
Laboratoire Ecologie Fonctionnelle et Environnement (UMR5245, Université de Toulouse, France)
Toulouse
FR
Klara Martinez Cruz
Universidad de Magallanes
Punta Arenas
CL
Armando Sepulveda Jaureguy
Universidad de Magallanes
Punta Arenas
CL
Céline Lavergne
Pontificia Universidad Católica de Valparaíso
Valparaiso
CL
Roman Teisserenc
Researcher
Laboratoire Ecologie Fonctionnelle et Environnement (UMR5245, Université de Toulouse, France)
Toulouse
FR
Polette Aguilar
Pontificia Universidad Católica de Valparaíso
Valparaíso
CL
Oscar Gerardo-Nieto
Cinvestav
Mexico City
MX
Claudia Etchebehere
Instituto de Investigaciones Biológicas Clemente Estable
Montevideo
UY
Bruna Martins
Instituto de Investigaciones Biológicas Clemente Estable
Montevideo
UY
Javier Fochesatto
University of Alaska Fairbanks
Fairbanks
US
Nikita Tananaev
P.I. Melnikov Permafrost Institute
Igarka
RU
Mette Svenning
UiT The Arctic University of Norway
Tromso
NO
Christophe Seppey
UiT The Arctic University of Norway
Tromso
NO
Alexander Tveit
UiT The Arctic University of Norway
Tromso
NO
Rolando Chamy
Pontificia Universidad Católica de Valparaíso
Valparaíso
CL
Maria Soledad Astorga-España
Universidad de Magallanes
Punta Arenas
CL
Andres Mansilla
Universidad de Magallanes
Punta Arenas
CL
Anton Van de Putte
Royal Belgian Institute for Natural Sciences
Brussels
BE
Maxime Sweetlove
Royal Belgian Institute for Natural Sciences
Brussels
BE
Alison Murray
Desert Research Institute
Reno
US
Léa Cabrol
Researcher
Institut Méditerranéen d’Océanologie
Marseille
FR

可回覆此資源相關問題者:

Maialen Barret
Associate professor
Laboratoire Ecologie Fonctionnelle et Environnement (UMR5245, Université de Toulouse, France)
Toulouse
FR
Léa Cabrol
Researcher
Institut méditerranéen d'Océanologie
Marseille
FR

元數據填寫者:
-

Maialen Barret
Associate professor
Laboratoire Ecologie Fonctionnelle et Environnement (UMR5245, Université de Toulouse, France)
Toulouse
FR

與此資源的相關者:

Maialen Barret

地理涵蓋範圍

Alaska

界定座標範圍 緯度南界 經度西界 [63.21, -150.8], 緯度北界 經度東界 [68.62, -147.65]

分類群涵蓋範圍

Bacterial and Archaea diversity was profiled by targeting the V4-V5 region of the 16S SSU rRNA gene for high throughput metabarcode (amplicon) sequencing, using the Illumia MiSeq platform (2x 250bp).

Domain Bacteria (Bacteria), Archaea (Archaea)

時間涵蓋範圍

起始日期 2016-06-27

計畫資料

METHANOgenic Biodiversity and activity in Arctic, subarctic and Subantarctic Ecosystems affected by climate change

計畫名稱 Methanobase
辨識碼 METHANOBASE ELAC2014-DCC092
經費來源 ERANET-LAC joint call 2014
研究區域描述 Alaska Lakes (water, sediments), peatlands (hollows, edges, hummocks) and mineral soils
研究設計描述 The METHANOBASE project has been designed to investigate the intricate relations between microbial diversity and methane emissions in Arctic, Subarctic and Subantarctic ecosystems, under natural (baseline) conditions and in response to simulated temperature increments.

參與計畫的人員:

Maialen Barret

取樣方法

Water samples were collected with a Van Dorn bottle. Sediments were sampled thanks to a grab-sampler, peat monoliths (approximately 30*30*30cm) were cut with a bread-knife and soil monoliths with a shovel.

研究範圍 Samples were collected in summer 2015, without any temporal replication. A total of 19 ecosystems were studied in Alaska, USA. The selected sites are representative of this Subantarctic region: lakes, peatlands, Nothofagus forest, pampa In each site, various samples were collected to take into account the local heterogeneity: different depths in water column and sediments, soil horizons, hollows/edges/hummocks.

方法步驟描述:

  1. After collection, samples were stored at 4°C prior to further processing. Liquid samples were filtered at 0.45µm until clogging and the filters were stored at -20°C. DNA was extracted from these filters using the PowerWater DNA isolation kit (MOBIO) while DNA was extracted from solid samples using the PowerSoil DNA isolation kit (MOBIO). DNA extracts were kept at -20°C. The V4-V5 region of 16S rRNA gene was amplified in the following conditions: 515F and 928R primers (Wang & Qian, 2009. doi:10.1371/journal.pone.0007401), 2min at 94°C, 30 cycles of 60s at 94°C, 40s at 65°C and 30s at 72°C, and 10 min at 72°C. Amplicon sequencing was carried out with Illumina MiSeq technology (2x250pb, V3). Denoising of the sequences dataset and OTU clustering was carried using the FROGS pipeline (Auer et al., 2017. doi:10.1093/bioinformatics/btx791). BLAST was used for taxonomic affiliation.

額外的詮釋資料

替代的識別碼 0ea51b6e-d02f-495e-a29b-73783e4060c0
https://ipt.biodiversity.aq/resource?r=methanobasealaska