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Bacteria and Archaea biodiversity in Arctic and Subarctic terrestrial ecosystems in Alaska
Версия 1.2 опубликована SCAR - Microbial Antarctic Resource System Mar 19, 2019
Methane emissions from aquatic and terrestrial ecosystems play a crucial role in global warming, which is particularly affecting high-latitude ecosystems. As major contributors to methane emissions in natural environments, the microbial communities involved in methane production and oxidation deserve a special attention. Microbial diversity and activity are expected to be strongly affected by the already observed (and further predicted) temperature increase in high-latitude ecosystems, eventually resulting in disrupted feedback methane emissions. The METHANOBASE project has been designed to investigate the intricate relations between microbial diversity and methane emissions in Arctic, Subarctic and Subantarctic ecosystems, under natural (baseline) conditions and in response to simulated temperature increments. We report here a small subunit ribosomal RNA (16S rRNA) analysis of lake, peatland and mineral soil ecosystems.
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Cabrol L, Barret M, Thalasso F, Gandois L, Lavergne C, Martinez Cruz K, Sepulveda Jaureguy A, Fochesatto G J, Etchebehere C (2018): Bacteria and Archaea biodiversity in Arctic and Subarctic terrestrial ecosystems in Alaska. v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=methanobasealaska&v=1.2
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Публикующей организацией и владельцем прав на данную работу является SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License.
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Этот ресурс был зарегистрирован в GBIF, ему был присвоен следующий UUID: 0ea51b6e-d02f-495e-a29b-73783e4060c0. SCAR - Microbial Antarctic Resource System отвечает за публикацию этого ресурса, и зарегистрирован в GBIF как издатель данных при оподдержке Scientific Committee on Antarctic Research.
Ключевые слова
metadata; methane; greenhouse gas; bacteria; archaea; procaryote; peatland; wetland; soil; lake; sediment; metabarcoding; 16S rRNA; MiSeq
Контакты
Кто является создателем ресурса:
Кто может ответить на вопросы о ресурсе:
Кем заполнены метаданные:
Кто еще связан с данным ресурсом:
Географический охват
Alaska
| Ограничивающие координаты | Юг Запад [63.21, -150.8], Север Восток [68.62, -147.65] |
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Таксономический охват
Bacteria and Archaea
Временной охват
| Дата начала | 2016-06-27 |
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Данные проекта
METHANOgenic Biodiversity and activity in Arctic, subarctic and Subantarctic Ecosystems affected by climate change
| Название | Methanobase |
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| Идентификатор | METHANOBASE ELAC2014-DCC092 |
| Финансирование | ERANET-LAC joint call 2014 |
| Описание района исследования | Alaska Lakes (water, sediments), peatlands (hollows, edges, hummocks) and mineral soils |
| Описание плана выполнения исследований | The METHANOBASE project has been designed to investigate the intricate relations between microbial diversity and methane emissions in Arctic, Subarctic and Subantarctic ecosystems, under natural (baseline) conditions and in response to simulated temperature increments. |
Исполнители проекта:
Методы сбора
Water samples were collected with a Van Dorn bottle. Sediments were sampled thanks to a grab-sampler, peat monoliths (approximately 30*30*30cm) were cut with a bread-knife and soil monoliths with a shovel.
| Охват исследования | Samples were collected in summer 2015, without any temporal replication. A total of 19 ecosystems were studied in Alaska, USA. The selected sites are representative of this Subantarctic region: lakes, peatlands, Nothofagus forest, pampa In each site, various samples were collected to take into account the local heterogeneity: different depths in water column and sediments, soil horizons, hollows/edges/hummocks. |
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Описание этапа методики:
- After collection, samples were stored at 4°C prior to further processing. Liquid samples were filtered at 0.45µm until clogging and the filters were stored at -20°C. DNA was extracted from these filters using the PowerWater DNA isolation kit (MOBIO) while DNA was extracted from solid samples using the PowerSoil DNA isolation kit (MOBIO). DNA extracts were kept at -20°C. The V4-V5 region of 16S rRNA gene was amplified in the following conditions: 515F and 928R primers (Wang & Qian, 2009. doi:10.1371/journal.pone.0007401), 2min at 94°C, 30 cycles of 60s at 94°C, 40s at 65°C and 30s at 72°C, and 10 min at 72°C. Amplicon sequencing was carried out with Illumina MiSeq technology (2x250pb, V3). Denoising of the sequences dataset and OTU clustering was carried using the FROGS pipeline (Auer et al., 2017. doi:10.1093/bioinformatics/btx791). BLAST was used for taxonomic affiliation.
Дополнительные метаданные
| Альтернативные идентификаторы | https://ipt.biodiversity.aq/resource?r=methanobasealaska |
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