Bacteria and Archaea biodiversity in terrestrial ecosystems of Chilean Patagonia

Versão mais recente published by SCAR - Microbial Antarctic Resource System on abr 15, 2022 SCAR - Microbial Antarctic Resource System
Publication date:
15 de abril de 2022
Licença:
CC-BY 4.0

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Descrição

Methane emissions from aquatic and terrestrial ecosystems play a crucial role in global warming, which is particularly affecting high-latitude ecosystems. As major contributors to methane emissions in natural environments, the microbial communities involved in methane production and oxidation deserve a special attention. Microbial diversity and activity are expected to be strongly affected by the already observed (and further predicted) temperature increase in high-latitude ecosystems, eventually resulting in disrupted feedback methane emissions. The METHANOBASE project has been designed to investigate the intricate relations between microbial diversity and methane emissions in Arctic, Subarctic and Subantarctic ecosystems, under natural (baseline) conditions and in response to simulated temperature increments. We report here a small subunit ribosomal RNA (16S rRNA) analysis of lake, peatland and mineral soil ecosystems.

Versões

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Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Barret M, Thalasso F, Gandois L, Martinez Cruz K, Sepulveda Jaureguy A, Lavergne C, Teisserenc R, Aguilar P, Gerardo-Nieto O, Etchebehere C, Martins B, Fochesatto J, Tananaev N, Svenning M, Seppey C, Tveit A, Chamy R, Soledad Astorga-España M, Mansilla A, Van de Putte A, Sweetlove M, Murray A, Cabrol L (2022): Bacteria and Archaea biodiversity in terrestrial ecosystems of Chilean Patagonia. v1.3. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=methanobasepatagonia&v=1.3

Direitos

Pesquisadores devem respeitar a seguinte declaração de direitos:

O editor e o detentor dos direitos deste trabalho é SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: d98b5000-4d67-4580-a90c-c818452cedcb.  SCAR - Microbial Antarctic Resource System publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por Scientific Committee on Antarctic Research.

Palavras-chave

methane; greenhouse gas; bacteria; archaea; procaryote; peatland; wetland; soil; lake; sediment; metabarcoding; 16S rRNA; MiSeq; Occurrence; Metadata

Contatos

Maialen Barret
  • Provedor Dos Metadados
  • Autor
  • Originador
  • Ponto De Contato
Associate professor
Laboratoire Ecologie Fonctionnelle et Environnement (UMR5245, Université de Toulouse, France)
Toulouse
FR
Frederic Thalasso
  • Originador
Cinvestav
Mexico City
MX
Laure Gandois
  • Originador
Laboratoire Ecologie Fonctionnelle et Environnement (UMR5245, Université de Toulouse, France)
Toulouse
FR
Karla Martinez Cruz
  • Originador
Universidad de Magallanes
Punta Arenas
CL
Armando Sepulveda Jaureguy
  • Originador
Universidad de Magallanes
Punta Arenas
CL
Céline Lavergne
  • Originador
Pontificia Universidad Católica de Valparaíso
Valparaíso
CL
Roman Teisserenc
  • Originador
Laboratoire Ecologie Fonctionnelle et Environnement (UMR5245, Université de Toulouse, France)
Toulouse
FR
Polette Aguilar
  • Originador
Pontificia Universidad Católica de Valparaíso
Valparaíso
CL
Oscar Gerardo-Nieto
  • Originador
Cinvestav
Mexico City
MX
Claudia Etchebehere
  • Originador
Instituto de Investigaciones Biológicas Clemente Estable
Montevideo
UY
Bruna Martins
  • Originador
Instituto de Investigaciones Biológicas Clemente Estable
Montevideo
UY
Javier Fochesatto
  • Originador
University of Alaska Fairbanks
Fairbanks
US
Nikita Tananaev
  • Originador
P.I. Melnikov Permafrost Institute
Igarka
RU
Mette Svenning
  • Originador
UiT The Arctic University of Norway
Tromso
NO
Christophe Seppey
  • Originador
UiT The Arctic University of Norway
Tromso
NO
Alexander Tveit
  • Originador
UiT The Arctic University of Norway
Tromso
NO
Rolando Chamy
  • Originador
Pontificia Universidad Católica de Valparaíso
Valparaíso
CL
Maria Soledad Astorga-España
  • Originador
Universidad de Magallanes
Punta Arenas
CL
Andres Mansilla
  • Originador
Universidad de Magallanes
Pnta Arenas
CL
Anton Van de Putte
  • Originador
Royal Belgian Institute for Natural Sciences
Brussels
BE
Maxime Sweetlove
  • Originador
Royal Belgian Institute for Natural Sciences
Brussels
BE
Alison Murray
  • Originador
Desert Research Institute
Reno
US
Léa Cabrol
  • Provedor Dos Metadados
  • Originador
  • Ponto De Contato
Institut Méditerranéen d’Océanologie
Marseille
FR

Cobertura Geográfica

Chilean Patagonia

Coordenadas delimitadoras Sul Oeste [-54,065, -72,038], Norte Leste [-52,073, -68,85]

Cobertura Taxonômica

Bacterial and Archaea diversity was profiled by targeting the V4-V5 region of the 16S SSU rRNA gene for high throughput metabarcode (amplicon) sequencing, using the Illumia MiSeq platform (2x 250bp).

Domínio Bacteria (Bacteria), Archaea (Archaea)

Cobertura Temporal

Data Inicial 2016-01-13

Dados Sobre o Projeto

METHANOgenic Biodiversity and activity in Arctic, subarctic and Subantarctic Ecosystems affected by climate change

Título Methanobase
Identificador METHANOBASE ELAC2014-DCC092
Financiamento ERANET-LAC joint call 2014
Descrição da Área de Estudo Patagonia [-54.94925011 to -52.0728575, -72.03831167 to -67.33177633] Lakes (water, sediments), peatlands (hollows, edges, hummocks) and mineral soils
Descrição do Design The METHANOBASE project has been designed to investigate the intricate relations between microbial diversity and methane emissions in Arctic, Subarctic and Subantarctic ecosystems, under natural (baseline) conditions and in response to simulated temperature increments.

O pessoal envolvido no projeto:

Maialen Barret
  • Pesquisador Principal
Léa Cabrol
  • Pesquisador Principal
Frederic Thalasso
  • Provedor Dos Metadados
Karla Martinez Cruz
  • Provedor Dos Metadados
Armando Sepulveda Jaureguy
  • Provedor Dos Metadados
Laure Gandois
  • Provedor Dos Metadados
Roman Teisserenc
  • Autor
Alison Murray
Anton Van de Putte

Métodos de Amostragem

Water samples were collected with a Van Dorn bottle. Sediments were sampled thanks to a grab-sampler, peat monoliths (approximately 30*30*30cm) were cut with a bread-knife and soil monoliths with a shovel.

Área de Estudo Samples were collected in summer 2015, without any temporal replication. A total of 19 ecosystems were studied in Alaska, USA. The selected sites are representative of this Subantarctic region: lakes, peatlands, Nothofagus forest, pampa In each site, various samples were collected to take into account the local heterogeneity: different depths in water column and sediments, soil horizons, hollows/edges/hummocks.

Descrição dos passos do método:

  1. After collection, samples were stored at 4°C prior to further processing. Liquid samples were filtered at 0.45µm until clogging and the filters were stored at -20°C. DNA was extracted from these filters using the PowerWater DNA isolation kit (MOBIO) while DNA was extracted from solid samples using the PowerSoil DNA isolation kit (MOBIO). DNA extracts were kept at -20°C. The V4-V5 region of 16S rRNA gene was amplified in the following conditions: 515F and 928R primers (Wang & Qian, 2009. doi:10.1371/journal.pone.0007401), 2min at 94°C, 30 cycles of 60s at 94°C, 40s at 65°C and 30s at 72°C, and 10 min at 72°C. Amplicon sequencing was carried out with Illumina MiSeq technology (2x250pb, V3). Denoising of the sequences dataset and OTU clustering was carried using the FROGS pipeline (Auer et al., 2017. doi:10.1093/bioinformatics/btx791). BLAST was used for taxonomic affiliation.

Metadados Adicionais

Identificadores alternativos d98b5000-4d67-4580-a90c-c818452cedcb
https://ipt.biodiversity.aq/resource?r=methanobasepatagonia