Microorganisms (eukaryote and bacteria) in Antarctic cryoconite holes.

Versão mais recente published by SCAR - Microbial Antarctic Resource System on mar 19, 2019 SCAR - Microbial Antarctic Resource System
Publication date:
19 de Março de 2019
Licença:
CC-BY 4.0

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Descrição

Amplicon sequencing dataset (Illumina MiSeq) of Eukaryotes (18S ssu rRNA) and Bacterial (16S ssu rRNA) microbes in Antarctic cryoconite holes.

Versões

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Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Sommers P, Darcy J, Gendrom E, Stanish L, Bagshaw E, Porazinska D, Schmidt S (2019): Microorganisms (eukaryote and bacteria) in Antarctic cryoconite holes.. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbes_antarctic_cryoconite&v=1.1

Direitos

Pesquisadores devem respeitar a seguinte declaração de direitos:

O editor e o detentor dos direitos deste trabalho é SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 3adb4fd7-9e77-41e0-aa37-9ca4b72c5eaa.  SCAR - Microbial Antarctic Resource System publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por Scientific Committee on Antarctic Research.

Palavras-chave

Metadata

Contatos

Pacifica Sommers
  • Originador
  • Ponto De Contato
University of Colorado at Boulder
Boulder
US
John Darcy
  • Originador
University of Hawaï at Manoa
Manoa
US
Eli Gendrom
  • Originador
University of Colorado at Boulder
Boulder
US
Lee Stanish
  • Originador
National Ecological Observatory Network
Boulder
US
Elizabeth Bagshaw
  • Originador
Cardiff University
Cardiff
GB
Dorota Porazinska
  • Originador
University of Colorado at Boulder
Boulder
US
Steven Schmidt
  • Originador
University of Colorado at Boulder
Boulder
US
Maxime Sweetlove
  • Provedor Dos Metadados
Research assistent
Royal Belgian Institute of Natural Sciences
Rue Vautier 29
1000 Brussels

Cobertura Geográfica

Cryoconite holes in Taylor Valley, Antarctica

Coordenadas delimitadoras Sul Oeste [-77,615, 162,967], Norte Leste [-77,615, 162,967]

Cobertura Taxonômica

Bacteria (16S ssu rRNA gene)

Domínio Bacteria (Bacteria)

Eukaryotes (18S ssu rRNA gene)

Domínio Eukaryota (Eukaryotes)

Cobertura Temporal

Data Inicial 2007-01-01

Dados Sobre o Projeto

Nenhuma descrição disponível

Título Antarctic cryoconite 2007
Financiamento This work was funded by NSF Polar Programs Award 1443578 and by Duke University.

O pessoal envolvido no projeto:

Pacifica Sommers

Métodos de Amostragem

A core was collected from the center of each hole using a SIPRE corer. The core was removed and stored in a Ziploc bag that had been triple-rinsed with deionized water. For the samples in which meltwater was present at the time of sampling, the ice lid was removed with the SIPRE corer, and then a water sample was pumped out using a hand powered vacuum pump. A sample of sediment was removed and stored in a triple-rinsed Ziploc bag. On return to the eld laboratory, samples were stored at –20◦C until processing up to 30 days later. Samples were eventually allowed to melt out in the collection bags and water samples were drawn off using syringes, leaving the sediment behind.

Área de Estudo Nineteen cryoconite holes on glaciers in Taylor Valley (McMurdo Dry Valleys, Antarctica) were sampled between 15 December 2007 and 4 January 2008.

Descrição dos passos do método:

  1. All cryoconite samples were stored frozen at –70◦C to minimize DNA degradation. Between 25 April 2016 and 2 May 2016, the frozen cryoconite sediments were thawed at room temperature and 0.4 g was processed for DNA extraction from each technical replicate separately (47 total from 19 samples) using PowerSoil DNA Isolation Kits (MoBio Inc., Carlsbad, CA, USA), according to the manufacture’s protocol. Extracted genomic DNA was amplified in triplicate using 16S (515f-806r primers) and 18S (1391f-EukBr primers) SSU ribosomal gene markers. Amplified DNA was pooled and normalized to equimolar concentrations using SequalPrep Normalization Plate Kits (Invitrogen), and sequenced using the Illumina MiSeq V2 (2 × 250 bp chemistry) at the BioFrontiers Sequencing Core Facility at the University of Colorado at Boulder.

Citações bibliográficas

  1. Sommers, P., Darcy, J. L., Gendron, E. M., Stanish, L. F., Bagshaw, E. A., Porazinska, D. L., & Schmidt, S. K. (2017). Diversity patterns of microbial eukaryotes mirror those of bacteria in Antarctic cryoconite holes. FEMS microbiology ecology, 94(1), fix167.

Metadados Adicionais

Identificadores alternativos 3adb4fd7-9e77-41e0-aa37-9ca4b72c5eaa
https://ipt.biodiversity.aq/resource?r=microbes_antarctic_cryoconite