Microbes (Eukaryotes and Archaea) in sea water from Fildes Peninsula (King George Island, Antarctica)

Последняя версия опубликовано SCAR - Microbial Antarctic Resource System мар 19, 2019 SCAR - Microbial Antarctic Resource System
Дата публикации:
19 марта 2019 г.
Опубликовано:
SCAR - Microbial Antarctic Resource System
Лицензия:
CC-BY 4.0

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Описание

Amplicon sequencing dataset (Illumina MiSeq) of microbial Eukaryotes (18S ssu rRNA gene), and Archaea in sea water samples taken during the 29th Chinese Antarctic scientific expedition in 2013 at Greatwall cove and Ardley cove, Fildes Peninsula (King George Island, Antarctica).

Версии

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Как оформить ссылку

Исследователи должны дать ссылку на эту работу следующим образом:

Luo W, Li H, Gao S, Yu Y, Lin L, Zeng T (2019): Microbes (Eukaryotes and Archaea) in sea water from Fildes Peninsula (King George Island, Antarctica). v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbes_fildes_peninsula_antarctica&v=1.2

Права

Исследователи должны соблюдать следующие права:

Публикующей организацией и владельцем прав на данную работу является SCAR - Microbial Antarctic Resource System. Эта работа находится под лицензией Creative Commons Attribution (CC-BY 4.0).

Регистрация в GBIF

Этот ресурс был зарегистрирован в GBIF, ему был присвоен следующий UUID: a4af5ceb-4035-49f5-b41a-bb548307b4f8.  SCAR - Microbial Antarctic Resource System отвечает за публикацию этого ресурса, и зарегистрирован в GBIF как издатель данных при оподдержке Scientific Committee on Antarctic Research.

Ключевые слова

Metadata

Контакты

Wei Luo
  • Originator
  • Point Of Contact
Polar Research Institute of China
Shanghai
CN
Huirong Li
  • Originator
Polar Research Institute of China
Shanghai
CN
Shengquan Gao
  • Originator
Second Institute of Oceanography
Hangzhou
CN
Yong Yu
  • Originator
Polar Research Institute of China
Shanghai
CN
Ling Lin
  • Originator
Polar Research Institute of China
Shanghai
CN
Tinxin Zeng
  • Originator
Polar Research Institute of China
Shanghai
CN
Maxime Sweetlove
  • Metadata Provider
Research assistent
Royal Belgian Institute of Natural Sciences
Rue Vautier 29
1000 Brussels

Географический охват

Fildes Peninsula, King George Island, Antarctica

Ограничивающие координаты Юг Запад [-62,2, -58,9], Север Восток [-62,2, -58,9]

Таксономический охват

microbial Eukaryotes were sampled based on marker gene amplification (18S ssu rRNA gene)

Domain Eukaryota (Eukaryotes)

Archaea were sampled based on marker gene amplification

Domain Archaea (Archaea)

Временной охват

Дата начала / Дата окончания 2013-01-17 / 2013-01-23

Данные проекта

Описание отсутсвует

Название Microbes (Eukaryotes and Archaea) in sea water from Fildes Peninsula (King George Island, Antarctica)
Финансирование This work was supported by the National High-Tech Research and Development Program of China (Grant Nos. 2012AA021706, 2013AA065805), National Natural Science Foundation of China (No. 41376191), Chinese Polar Environment Comprehensive Investigation and Assessment Program (CHINARE2014-02-01), and Shanghai Rising-Star Program (11QA1407300).

Исполнители проекта:

Wei Luo

Методы сбора

1 L of surface sea water from each station was collected and prefiltered through a 20-µm mesh sieve to remove most of the mesozooplankton and large particles, and then directly filtered through a 0.2-µm pore size nucleopore membrane filter (Whatman). The filters were frozen at −80 °C in cetyltrimethylammonium bromide (CTAB) buffer until laboratory experiments were carried out.

Охват исследования Samples were taken in January 2013, during the 29th Chinese National Antarctic Research Expedition at Greatwall Cove and Ardley Cove (king George Island, Antarctica)

Описание этапа методики:

  1. DNA extraction was performed as described by Luo et al. (2009).
  2. Polymerase chain reaction (PCR) was performed using primers with barcodes flanking the hypervariable V4 region of the 18S rRNA gene: 3NDf with the reverse primer V4_euk_R2. PCR was conducted in 20 μL reactions with 0.2 μM each of the primers, ~10 ng of template DNA, 1 × PCR buffer, and 2.5 U of Pfu DNA Polymerase (Promega, USA). The amplification programme consisted of an initial denaturation step at 95 °C for 2 min, followed by 30 cycles at 95 °C for 30 s, 55 °C for 30 s, and 72 °C for 30 s, and a final extension of 72 °C for 5 min. PCR products were pooled and purified using a DNA gel extraction kit (Axygen, Hangzhou, China). The DNA concentration of each PCR product was determined using a Quant-iT PicoGreen double-stranded DNA assay (Invitrogen, Germany) and was quality controlled on a TBS-380 Mini-Fluorometer (Turner Biosystems, Sunnyvale, CA, USA). Finally, amplicons of all samples were pooled in equimolar concentrations.
  3. 18S rRNA amplification and sequencing on the Illumina MiSeq2000 were done by following the standard protocols of Earth Microbiome Project (EMP) (Caporaso et al. 2012).

Библиографические ссылки

  1. Luo, W., Li, H., Gao, S., Yu, Y., Lin, L., & Zeng, Y. (2016). Molecular diversity of microbial eukaryotes in sea water from Fildes Peninsula, King George Island, Antarctica. Polar Biology, 39(4), 605-616. https://doi.org/10.1007/s00300-015-1815-8

Дополнительные метаданные

Альтернативные идентификаторы a4af5ceb-4035-49f5-b41a-bb548307b4f8
https://ipt.biodiversity.aq/resource?r=microbes_fildes_peninsula_antarctica