Microorganisms in frost flowers on young Arctic sea ice, comparison between different ice types

Dernière version Publié par SCAR - Microbial Antarctic Resource System le mars 21, 2019 SCAR - Microbial Antarctic Resource System
Date de publication:
21 mars 2019
Licence:
CC-BY 4.0

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Description

Amplicon sequencing dataset (454 pyrosequencing) of Bacteria in different types of young sea ice and sea ice brines in the Arctic ocean (North-East coast of Greenland)

Versions

Le tableau ci-dessous n'affiche que les versions publiées de la ressource accessibles publiquement.

Comment citer

Les chercheurs doivent citer cette ressource comme suit:

Barber D, Ehn J, Pucko M, Rysgaard S, Deming J, Bowman J, Papakyriakou T, Galley R, Sogaard D (2019): Microorganisms in frost flowers on young Arctic sea ice, comparison between different ice types. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microorganisms_in_frost_flowers_on_young_arctic_sea_ice&v=1.0

Droits

Les chercheurs doivent respecter la déclaration de droits suivante:

L’éditeur et détenteur des droits de cette ressource est SCAR - Microbial Antarctic Resource System. Ce travail est sous licence Creative Commons Attribution (CC-BY) 4.0.

Enregistrement GBIF

Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : 5953581e-6345-48ce-9887-1783808c7cde.  SCAR - Microbial Antarctic Resource System publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du Scientific Committee on Antarctic Research.

Mots-clé

Metadata

Contacts

David Barber
  • Créateur
  • Personne De Contact
University of Manitoba
Winnipeg
CA
J.K. Ehn
  • Créateur
University of Manitoba
Winnipeg
CA
M. Pucko
  • Créateur
University of Manitoba
Winnipeg
CA
S. Rysgaard
  • Créateur
University of Manitoba
Winnipeg
CA
J.W. Deming
  • Créateur
University of Washington
Seattle
US
J.S. Bowman
  • Créateur
University of Washington
Seattle
US
T. Papakyriakou
  • Créateur
University of Manitoba
Winnipeg
CA
R.J. Galley
  • Créateur
University of Manitoba
Winnipeg
CA
D.H. Sogaard
  • Créateur
Greenland Institute of Natural Resources
Nuuk
GL
Maxime Sweetlove
  • Fournisseur Des Métadonnées
Research assistent
Royal Belgian Institute of Natural Sciences
Rue Vautier 29
1000 Brussels
BE

Couverture géographique

Young Sound, North-East Greenland

Enveloppe géographique Sud Ouest [74,468, -20,311], Nord Est [74,468, -20,311]

Couverture taxonomique

Bacteria were profiled by targeting the 16S ssu rRNA gene (v3-v5 region)

Domain Bacteria (Bacteria)

Couverture temporelle

Epoque de formation 2012-03

Données sur le projet

Pas de description disponible

Titre Microorganisms in frost flowers on young Arctic sea ice, comparison between different ice types
Financement Canada Excellence Research Chair (CERC) and Canada Research Chair programs, the Natural Sciences and Engineering Research Council, the Canada Foundation for Innovation, the U.S. National Science Foundation (award OPP‐ARC1205152) and Walters Endowed Professorship (J.W.D.).

Les personnes impliquées dans le projet:

David Barber

Méthodes d'échantillonnage

Frost flowers for microbial analyses were removed from the pond site into sterile 1 L plastic bags using an ethanol‐rinsed spatula. A second scraping over the same surface area yielded the corresponding, operationally defined brine skim, i.e., the surface slush layer. Samples of sea ice were also collected, as described above, along with the samples of seawater and snow from the surrounding area. Samples of frost flowers, the underlying surface slush layer, and snow were melted directly over the shortest possible period (always <12 h, with sample temperature remaining at ≤0°C), while sea ice samples were melted into sterile 0.2 µm filtered brine according to the isohaline approach described by Ewert et al. [2013].

Etendue de l'étude The thin‐ice station, POLY I (74°13.905′N, 20°07.701′W, 29–30 cm thick on 22 March, snow covered with varying thickness), was situated in a recurrent winter polynya region about 3 km off the landfast ice edge.

Description des étapes de la méthode:

  1. An area of ~2.5 × 7 m was opened near POLY I at 16:00 geomagnetic time (GMT) on 22 March to expose the ocean to the atmosphere (hereinafter referred to as the “pond” site). The opening of the pond was done using a handheld ice saw by cutting smaller segments that then were pushed to the side underneath the ice cover. A time‐lapse camera was installed at the pond site to document the development of frost flowers as the ice formed in situ. Half of the pond was reopened on 24 March at 15:00 GMT, i.e., after about 47 h of the initial pond opening. At this time, the initial ice was ~12 cm thick. The recurrent polynya at this location will occur as open water (as evidenced by satellite imagery just prior to our arrival) or with a young ice cover (like we experienced); frost flowers are known to occur regularly on this polynya ice.
  2. DNA was extracted from the different sample types for amplification and sequencing of the 16S ribosomal ribonucleic acid (rRNA) gene using the phenol‐chloroform method, as in Bowman et al. (2013). One patch of frost flowers was sampled to obtain the upper centimeter portions separately from the basal portions. The V3–V5 regions of the 16S rRNA gene were amplified using primers 357F and 926R for 30 cycles. An aliquot of the amplified material, along with positive and negative controls, was visualized on a gel to insure proper fragment length. Amplicons were purified using the GeneJet Purification Kit (Fermentas) and submitted to the Tufts University Sequencing Center, where amplicons underwent a second round amplification for 10 cycles using barcoded primers 517F and 967R. Second round amplicons were gel purified prior to library construction. Sequencing was conducted on the 454 FLX platform (Roche) using titanium chemistry.

Citations bibliographiques

  1. Barber, D. G., Ehn, J. K., Pućko, M., Rysgaard, S., Deming, J. W., Bowman, J. S., ... & Søgaard, D. H. (2014). Frost flowers on young Arctic sea ice: The climatic, chemical, and microbial significance of an emerging ice type. Journal of Geophysical Research: Atmospheres, 119(20), 11-593. https://doi.org/10.1002/2014JD021736