Stable isotope ratios and elemental contents of C, N and S in benthic organisms sampled during the PROTEKER 5 summer campaign in Kerguelen Islands (2016) - data

資料紀錄

此資源sampling event的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 37 筆紀錄。

亦存在 2 筆延伸集的資料表。延伸集中的紀錄補充核心集中紀錄的額外資訊。 每個延伸集資料表中資料筆數顯示如下。

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版本

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如何引用

研究者應依照以下指示引用此資源。:

Le Bourg B, Saucède T, Charpentier A, Lepoint G, Gan Y, Michel L N (2022): Stable isotope ratios and elemental contents of C, N and S in benthic organisms sampled during the PROTEKER 5 summer campaign in Kerguelen Islands (2016) - data. v1.4. SCAR - AntOBIS. Dataset/Samplingevent. https://ipt.biodiversity.aq/resource?r=proteker2016-event&v=1.4

權利

研究者應尊重以下權利聲明。:

此資料的發布者及權利單位為 SCAR - AntOBIS。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF 註冊

此資源已向GBIF註冊，並指定以下之GBIF UUID: 28120c52-2b34-4db4-b348-812ee1eaf958。  SCAR - AntOBIS 發佈此資源，並經由Ocean Biodiversity Information System同意向GBIF註冊成為資料發佈者。

關鍵字

Occurrence; CRUSTACEANS; DECAPODS; AMPHIPODS; ISOPODS; ARTHROPODS; ECHINODERMS; SEA STARS; SEA URCHINS; BIVALVES; MUSSELS; GASTROPODS; SEGMENTED WORMS (ANNELIDS); SPONGES; ANIMALS/INVERTEBRATES; MACROALGAE (SEAWEEDS); BROWN ALGAE; GREEN ALGAE; RED ALGAE; BIODIVERSITY FUNCTIONS; FOOD-WEB DYNAMICS; TROPHIC DYNAMICS; MARINE ECOSYSTEMS; BENTHIC; COASTAL; KELP FOREST; STABLE ISOTOPES; ORGANIC MATTER; SEDIMENTS; SUSPENDED SOLIDS

聯絡資訊

地理涵蓋範圍

Kerguelen Island, Gulf of Morbihan, close to Port-aux-Français scientific station

分類群涵蓋範圍

無相關描述

時間涵蓋範圍

計畫資料

Title: Refugia and ecosystem tolerance in the Southern Ocean Identifier: RECTO Description: More info at https://www.belspo.be/belspo/brain-be/projects/RECTO_en.pdf Funding: This project was funded by the Belgian Federal Science Policy (BELSPO) under the BRAIN-be scheme. Grant number: BR/154/A1/RECTO. Title: Ecosystem Responses to Global change: a multiscale approach in the Southern Ocean Identifier: vERSO Description: More info at https://www.belspo.be/belspo/brain-be/projects/vERSO_en.pdf Funding: This project was funded by the Belgian Federal Science Policy (BELSPO) under the BRAIN-be scheme. Grant number: BR/132/A1/VERSO. Title: Effects of global change on coastal habitats of the Kerguelen Islands. Establishment of a baseline for ecological and genetic monitoring, protection and conservation Identifier: IPEV research program 1044 PROTEKER Description: More info at https://www.proteker.net/ Funding: This project is funded by the French Polar Institute Paul-Emile Victor (IPEV). Grant number: 1044. Title: Feeding of Antarctic asteroids: identification of trophic resources and investigation of trophic plasticity and diversity. Identifier: FRIA doctoral grant number 1.E091.16 Description: The Southern Ocean undergoes intense and contrasted impacts linked to climate change. Sea stars (Echinodermata: Asteroidea) are a key group of the Southern Ocean benthos, considered to be quite resistant to seawater temperature changes. However, other more indirect environmental changes, might induce important shifts in food web structure and functioning, that may affect sea stars trophic ecology. This project proposes to investigate the trophic ecology of Antarctic asteroids using isotope analyses. Funding: This project was funded by the Belgian Fund for Scientific Research (F.R.S.-FNRS). Grant number: 1.E091.16.

參與計畫的人員:

取樣方法

All samples were taken in or next to (within a few meters) coastal kelp forests in the vicinity of the Port-aux-Français scientific station. Most samples were hand collected by SCUBA divers between 5 and 13 m depth. They included benthic primary producers, large macrophytodetritus, surface sediments and biomass-dominant benthic macroinvertebrates belonging to various taxa and feeding guilds: sponges, bivalves, gastropods, sedentary and mobile polychaetes, arthropods and echinoderms. SCUBA diving sampling events were complemented with intertidal collections on the shore and in rock pools at low tide. Those samples also comprised macrophytodetritus (wrack algae) as well as invertebrate consumers. Finally, for suspended particulate organic matter (SPOM), subsurface seawater samples were taken using a Niskin bottle. Water was subsequently filtered (1.8 to 2.1 dm3) on pre-combusted (400°C, 4 h) Whatman GF/F filters, and retained SPOM was kept for analysis.

方法步驟描述:

1. Sample preservation: After diving, samples were placed in oxygenated water containers and transferred to the Port-aux-Français station lab facilities where they were processed.
2. Sample preparation: Samples were immediately dissected to retrieve the tissue selected for stable isotope analyses (cf. "measurementRemarks" field of the dataset), which was then dried at 60°C during 72 hours. Samples were then homogenised into powder prior to SIA using a mortar and a pestle or a mixer mill (MM301, Retsch, Haan, Germany) depending on toughness. Whenever necessary (cf. "preparations" field of the dataset), carbonates were removed from subsamples by exposing subsamples to 37 % hydrochloric acid vapor during 48 hours. Acidified subsamples were then kept at 60°C until further sample preparation.
3. Stable isotope analysis: The subsamples were then precisely weighed (ca 2.5-3 mg) in 5×8 tin cups with ca 3 mg of tungsten trioxide. Their elemental composition and their stable isotope values were analyzed with an elemental analyser coupled to a continuous-flow isotope-ratio mass spectrometer. Isotopic ratios were expressed in ‰ using the widespread δ notation (Coplen 2011) and relative to the international references Vienna Pee Dee Belemnite (for carbon), Atmospheric Air (for nitrogen) and Vienna Canyon Diablo Troilite (for sulfur). Sucrose (IAEA-C-6; δ13C=−10.8 ± 0.5‰; mean ± standard deviation), ammonium sulfate (IAEA-N-1; δ15N= 0.4 ± 0.2‰; mean ± SD) and silver sulfide (IAEA-S-1; δ34S = −0.3‰) were used as primary analytical standards for stable isotope ratios. Sulfanilic acid (Sigma-Aldrich; δ13C=−25.6 ± 0.4‰; δ15N=−0.13 ± 0.4‰; δ34S = 5.9 ± 0.5‰; means ± SD) was used as a secondary analytical standard for stable isotope ratios and as elemental standard. Standard deviations on multi-batch replicate measurements of secondary and internal laboratory standards (sea star tegument), analyzed interspersed with samples (one replicate of each standard every 15 analyses), were 0.3‰ for δ13C and δ15N and 0.5‰ for δ34S.
4. Instrumentation: Stable isotope ratio measurements were performed by continuous flow–elemental analysis–isotope ratio mass spectrometry (CF-EA-IRMS) at University of Liège (Belgium), using a vario MICRO cube C-N-S elemental analyzer (Elementar Analysensysteme GmbH, Hanau, Germany) coupled to an IsoPrime100 isotope ratio mass spectrometer (Isoprime, Cheadle, United Kingdom).

引用文獻

1. Coplen, T.B. (2011), Guidelines and recommended terms for expression of stable-isotope-ratio and gas-ratio measurement results. Rapid Commun. Mass Spectrom., 25: 2538-2560. https://doi.org/10.1002/rcm.5129

額外的詮釋資料