Descripción
Amplicon sequencing dataset targeting eukaryote microorganisms (18S ssu rRNA marker gene) in permanently ice-covered lakes in the McMurdo Dry Valleys (Antarctica). Samples (n=89) were taken in the photic zone of the east lobe (ELB) and west lobe (WLB) of the chemically stratified Lake Bonney.
Versiones
La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.
¿Cómo referenciar?
Los usuarios deben citar este trabajo de la siguiente manera:
Bielewicz S, Bell E, Kong W, Froedberg I, Priscu J, Morgan-Kiss R (2021): Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=protist_diversity_in_antarctic_lake_during_polar_night&v=1.1
Derechos
Los usuarios deben respetar los siguientes derechos de uso:
El publicador y propietario de los derechos de este trabajo es SCAR - Microbial Antarctic Resource System. Esta obra está bajo una licencia Creative Commons de Atribución/Reconocimiento (CC-BY 4.0).
Registro GBIF
Este recurso ha sido registrado en GBIF con el siguiente UUID: 8d83b6ed-c88d-4bf6-90a2-c6ade46fee16. SCAR - Microbial Antarctic Resource System publica este recurso y está registrado en GBIF como un publicador de datos avalado por Scientific Committee on Antarctic Research.
Palabras clave
Metadata
Contactos
- Originador
- Originador
- Originador
- Originador
- Originador
- Originador ●
- Usuario ●
- Punto De Contacto
- Proveedor De Los Metadatos
Cobertura geográfica
Lake Bonney, McMurdo Dry Valleys, Antarctica West = East =
| Coordenadas límite | Latitud Mínima Longitud Mínima [-77,435, 161,405], Latitud Máxima Longitud Máxima [-77,316, 163,743] |
|---|
Cobertura temporal
| Fecha Inicial / Fecha Final | 2010-11-18 / 2011-03-15 |
|---|
Datos del proyecto
Mc Murdo Dry Valleys Long Term Ecological Research Project (LTER; http://www.mcmlter.org/)
| Título | Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition |
|---|---|
| Fuentes de Financiación | National Science Foundation (NSF) |
Personas asociadas al proyecto:
Métodos de muestreo
Samples (1 liter) were vacuum concentrated (5 kPa) onto 47 mm 0.45 µm Durapore polyvinylidene fluoride membrane filters (Millipore). The filters were frozen immediately in liquid nitrogen and transported frozen to McMurdo Station, where they were stored at -80 degrees C until DNA extraction.
| Área de Estudio | Lake Bonney in the McMurdo Dry Valleys (Antarctica). Samples were collected at 6, 13, 18, and 20m in the east lobe (ELB) and 10, 13, 15, and 20m in the west lobe (WLB) of Lake Bonney from February to April 2008. All collection depths are relative to the piezometric water level in the ice borehole. |
|---|
Descripción de la metodología paso a paso:
- DNA was isolated from a whole filter using the MP DNA kit (MP Biomedicals, CA) following the kit’s instruction. Sequencing was performed using the BigDye Terminator v3.1 cycle sequencing kit (ABI, CA) with M13R primer and the fragments were sequenced on an Applied Biosystems 3730×l DNA Analyzer (ABI, CA). The 18S rRNA gene sequences reported in the current study have been deposited in GenBank under accession numbers GU969060 to GU969102.
Referencias bibliográficas
- Bielewicz, S., Bell, E., Kong, W., Friedberg, I., Priscu, J. C., & Morgan-Kiss, R. M. (2011). Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition. The ISME Journal, 5(9), 1559–1564. doi:10.1038/ismej.2011.23
Metadatos adicionales
| Identificadores alternativos | https://ipt.biodiversity.aq/resource?r=protist_diversity_in_antarctic_lake_during_polar_night |
|---|