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V3-V4 16S rDNA of soil bacteria in King George Island and Deception Island

版本 1.0 由 SCAR - Microbial Antarctic Resource System 發佈於 Feb 1, 2019 SCAR - Microbial Antarctic Resource System

Amplicon sequencing dataset (Illumina MiSeq 250bp PE) of soil bacteria (16S ssu rRNA gene, v3-v4) on King George Island and Deception Island (Antarctica)

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如何引用

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Chua C, Yong S, Gonzalez M, Lavin P, Cheah Y, Tan G, Wong C (2019): V3-V4 16S rDNA of soil bacteria in King George Island and Deception Island. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. http://ipt.biodiversity.aq/resource?r=soil_bacteria_king_george_island_and_deception_island&v=1.0

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The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

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此資源已向GBIF註冊，並指定以下之GBIF UUID: fca3f5da-a6ae-464d-a488-38439546056d。  SCAR - Microbial Antarctic Resource System 發佈此資源，並經由Scientific Committee on Antarctic Research同意向GBIF註冊成為資料發佈者。

關鍵字

Metadata

聯絡資訊

資源建立者:

C. Chua
Universiti Malaysia Sabah 88400 Kota Kinabalu MY
S. Yong
Universiti Malaysia Sabah 88400 Kota Kinabalu MY
M. Gonzalez
Instituto Antártico Chileno Punta Arenas CL
P. Lavin
Instituto Antártico Chileno Punta Arenas CL
Y. Cheah
Universiti Putra Malaysia 43400 Serdang MY
G. Tan
University of Malaya 88400 Kuala Lumpur MY
C. Wong
Universiti Malaysia Sabah 88400 Kota Kinabalu MY

可回覆此資源相關問題者:

C. Chua
Universiti Malaysia Sabah 88400 Kota Kinabalu MY
C. Wong
Universiti Malaysia Sabah 88400 Kota Kinabalu MY

元數據填寫者:

Maxime Sweetlove
Research assistent
Royal Belgian Institute for Naturals Sciences Rue Vautier 29 1000 Brussels

與此資源的相關者:

使用者

地理涵蓋範圍

King George Island and Deception Island, Antarctica

界定座標範圍 緯度南界 經度西界 [-62.99, -60.68], 緯度北界 經度東界 [-62.19, -58.94]

分類群涵蓋範圍

Bacteria, 16S ssu rRNA gene, v3-v4 region

Domain  Bacteria (Bacteria)

計畫資料

無相關描述

計畫名稱 V3-V4 16S rDNA of soil bacteria in King George Island and Deception Island
經費來源 his work was supported by the Ministry of Science, Technology and Innovation (MOSTI), Malaysia under the Flagship project FP0712E012.

The personnel involved in the project:

C. Chua

取樣方法

Samples were stored at –20°C immediately after sampling.

研究範圍 Soil samples were collected from King George Island (S62°11′35.3′′; W58°56′6.2′′) and Deception Island (S62°59′023′′; W60°40′51.7′′), Antarctica in February 2007.

方法步驟描述:

  1. Total bacterial genomic DNA was extracted from soils using the method described in Zhou et al. (1996) DNA recovery from soils of diverse composition. Appl. Environ. Microbiol., 62(2), 316–322.
  2. The concentration and purity of the extracted genomic DNA were measured using the NanoDropTM 2000 spectrophotometer (Thermo Scientific Inc., Waltham, MA, USA).
  3. The V3–V4 region of the bacterial 16S rRNA gene was amplified using locus-specific primers, S-DBact-0341-b- S-17 (5′-TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG CCT ACG GGN GGC WGC AG-3′)and S-D-Bact-0785-a-A-21 (5′-GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GGA CTA CHV GGG TAT CTA ATC C-3′). The 16S rDNA amplicons were subjected to paired-end sequencing on Illumina MiSeq platform using 2 × 250 bp MiSeq Reagent Kit v2 (Illumina® Inc., San Diego, California).

引用文獻

  1. Chua, C. Y., Yong, S. T., González, M. A., Lavin, P., Cheah, Y. K., Tan, G. Y. A., & Wong, C. M. V. L. (2018). Analysis of bacterial communities of King George and Deception Islands, Antarctica using high-throughput sequencing. CURRENT SCIENCE, 115(9), 1701.