Bacteria on mummified seals in the Antarctic Dry Valleys

Grace Tiao1, Charles Lee1, Ian McDonald1, Donald Cowan2, Craig Cary1, Maxime Sweetlove3

1 University of Waikato, Hamilton, New Zealand; 2 University of the Western Cape, Cape Town, South Africa; 3 Royal Belgian Institute for Natural Sciences, Rue Vautier 29, 1000, Brussels

Corresponding author(s): Grace TiaoCharles LeeIan McDonaldDonald CowanCraig Cary, Maxime Sweetlove (msweetlove@naturalsciences.be)

Received {date}; Revised {date}; Accepted {date}; Published {date}

Citation: Combination of authors, year of data paper publication (in parentheses), Title, Journal Name, Volume, Issue number (in parentheses), and doi of the data paper.

Resource Citation

Tiao G, Lee C, McDonald I, Cowan D, Cary C (2019): Bacteria on mummified seals in the Antarctic Dry Valleys. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. http://ipt.biodiversity.aq/resource?r=antarctic_dry_valley_mummified_seal_microbiome&v=1.0

Abstract

Amplicon dataset of Bacteria (16S ssu rRNA) living around mummified seal carcasses in the Antarctic Dry Valleys 

Keywords: Metadata

Project details

Project title: Seal Movement
Personnel: Grace Tiao
Funding: This work was supported through grants from the New Zealand Foundation for Research Science and Technology, Antarctica New Zealand, and the National Science Foundation (OPP-0739648 and 0944560). Additional support was provided by a George Peabody Gardner Fellowship, by the New Zealand Foundation for Research, Science and Technology Postdoctoral Research Fellowship and the New Zealand Marsden Foundation (UOW1003).

Data published through GBIF: http://ipt.biodiversity.aq/resource?r=antarctic_dry_valley_mummified_seal_microbiome

Taxonomic coverage

General taxonomic coverage description: Amplicin sequencing of Bacteria (16S ssu rRNA gene)
Taxonomic ranks
Domain: Bacteria
Common names: Bacteria

Spatial coverage

General spatial coverage: soil samples from under and around mummified crabeater seal near Lake Miers (S 78°05′36″, E 163°51′22″), Miers Valley, Victoria Land, Antarctica, that was transported to a similar site 20 m north.
Coordinates: 78°55'60''S and 78°55'60''S Latitude; 163°51'22''E and 163°51'22''E Longitude 

Temporal coverage: January 15, 2005 - January 15, 2009

Methods

Method step description: 
- . 	16S rRNA gene were generated using the primer pair Tx9 (5′-GGATTAGAWACCCBGGTAGTC-3′) and 1391R (5′-GACGGGCRGTGWGTRCA-3′). PCR was performed in triplicate and pooled to reduce stochastic variability between reactions. Each 30 μl reaction contained 5 or 20 ng of extracted community DNA, Pfx polymerase and platinum polymerase (0.5 U each; Invitrogen), 1× Pfx PCR buffer with Pfx enhancer, 0.2 mM dNTPs, 1 mM MgCl2, 0.02 mg ml−1 BSA, 0.8 μM of forward and reverse primer, and PCR-grade water. Thermal cycling conditions were 94 °C for 2 min; 18 or 24 cycles of 94 °C for 15 s, 55 °C for 30 s and 68 °C for 1 min; and 68 °C for 3 min. 
- . 	Amplicons were size-selected and purified using polyacrylamide gel electrophoresis before being prepared for pyrosequencing by Taxon Biosciences (Tiburon, CA, USA).
Study extent description: On 30 January 2006, a mummified crabeater seal was carried from its original resting site near Lake Miers (S 78°05′36″, E 163°51′22″), Miers Valley, Victoria Land, Antarctica, to a new site of similar geomorphology 20 m north. Samples for amplicon sequencing were taken from the original site before transportation (austral summer 2005), the new site before transportation (control, austral summer 2006), and the new site after incubation (austral summer 2009)
Sampling description: Soil samples (>10 g each) were collected aseptically at a depth of 0.05m. Samples were stored in sterile plastic containers at −80 °C until analysis.

Metadata language: English
Date of metadata creation: 2019-01-03
Hierarchy level: Dataset

References
Tiao, G., Lee, C. K., McDonald, I. R., Cowan, D. A., & Cary, S. C. (2012). Rapid microbial response to the presence of an ancient relic in the Antarctic Dry Valleys. Nature Communications, 3, 660.