BCCM/ULC Cyanobacteria culture collection at the University of Liège (ISO9001 certified)

Annick Wilmotte1, Kim Beets2, Maxime Sweetlove3, Annick Wilmotte4, Paulette Charlier5

1 BCCM/ULC culture collection at the University of Liège, allée du 6 aout, 11, 4000, Liège, Belgium; 2 BCCM/ULC culture collection at the University of Liège, Institute of Chemistry B6 Sart Tilman, 4000, Liège, Belgium; 3 Royal Belgian Institute for Natural Sciences, Rue Vautier 29, 1000, Brussels, Belgium; 4 BCCM/ULC culture collection at the University of  Liège, Allée du 6 aout, 11, 4000, Liège, Belgium; 5 InBios-Centre for Protein Engineering, Allée du 6 aout, 11, 4000, Liège, Belgium

Corresponding author(s): Annick Wilmotte (BCCM.ULC@ulg.ac.be)Kim Beets, Maxime Sweetlove (msweetlove@naturalsciences.be), Annick Wilmotte (BCCM.ULC@ulg.ac.be)

Received {date}; Revised {date}; Accepted {date}; Published {date}

Citation: Combination of authors, year of data paper publication (in parentheses), Title, Journal Name, Volume, Issue number (in parentheses), and doi of the data paper.

Resource Citation

Wilmotte A, Beets K (2020): BCCM/ULC Cyanobacteria culture collection at the University of Liège (ISO9001 certified). v1.22. SCAR - Microbial Antarctic Resource System. Dataset/Occurrence. https://ipt.biodiversity.aq/resource?r=bccm_ulc_culture_collection_uliege&v=1.22

Abstract

BCCM/ULC is a small and dedicated public culture collection, currently containing one of the largest collections of documented (sub)polar cyanobacteria worldwide.
The BCCM/ULC collection is hosted by the Centre for Protein Engineering (the Unit) of the University of Liège. The host Unit is very active in research projects concerning the cyanobacterial diversity and biogeography, with a focus on polar biotopes. The approach used is polyphasic, including the isolation of strains and culture-independent methods (DGGE, clone libraries, pyrosequencing based on the ribosomal operon sequences). The participation to field expeditions in the Antarctic and Arctic has enabled to collect samples in many locations. Moreover, taxonomic research is carried out by the host Unit to improve the classification of the cyanobacterial phylum. It is based on a polyphasic approach combining the morphological and molecular characterizations of the strains.

Keywords: Occurrence

General description

Purpose: The data set was created to document the origin of the strains in the BCCM/ULC collection

Project details

Project title: Belgian Co-ordinated Collection of Micro-organisms (BCCM)
Personnel: Annick Wilmotte, Kim Beets
Funding: The BCCM coordination cell is part of the Research Programmes Department of the Belgian Science Policy office (Belspo). The Research Programmes department funds the BCCM consortium to support research, development and innovation in life sciences and biotechnology.

Belspo's integrated quality-environment management system is certified in accordance with the requirements of the ISO  9001 standard and registered in compliance with European regulation 1221/2009 (EMAS). The activities of the BCCM coordination cell are thus also guided by the principles of quality and environmental friendliness.
Study area descriptions/descriptor: global
Design description: The BCCM consortium is composed of 7 decentralised culture collections that are coordinated by a central team at the Belgian Science Policy Office. Each collection is part of a binomial with its host laboratory. The close cooperation between these two partners leads to an optimal balance between research and conservation activities.

Moreover, the cooperation between the collections leads to the exchange and implementation of best practices for the conservation of microbial genetic resources and the harmonised application of international standards and regulations in microbiology.

Thanks to the recurrent funding programme from the Belgian Science Policy Office, and with the support of their respective host institutes, about 70 people study and conserve the biodiversity present in the BCCM collections. As such, they contribute to research, development and innovation activities in biotechnology and life sciences.

Data published through GBIF: https://ipt.biodiversity.aq/resource?r=bccm_ulc_culture_collection_uliege

Taxonomic coverage

General taxonomic coverage description: The BCCM/ULC culture collection exclusively contains Cyanobacterial strains
Taxonomic ranks
Phylum: Cyanobacteria
Common names: Cyanobacteria

Spatial coverage

General spatial coverage: Global coverage, mainly Antarctica, Europe and the Americas
Coordinates: 89°54'0''S and 90°0'0''N Latitude; 180°0'0''W and 179°54'0''W Longitude 

Natural collections description

Parent collection identifier: BCCM
Collection name: BCCM/ULC Cyanobacteria Collection
Collection identifier: BCCM/ULC
Formation period: 1997-present
Specimen preservation method: No treatment
Specimen preservation method: Deep frozen

Methods

Method step description: Upon deposit, the depositors must fill the Accession form that includes the information on the origin and characteristics of the sampling site and of the srain isolated from the sampling site (http://bccm.belspo.be/docs/deposit-forms). These elements are used for the metadata.
Study extent description: The BCCM/ULC cyanobacteria culture collection accepts the deposits (public and safe) of all kinds of cyanobacteria, if they correspond to its cultivation capacities and are not duplicates of already existing strains.
Sampling description: The host laboratory has one of the largest research collections of documented (sub)polar cyanobacteria worldwide, with more than 100 strains characterized by phenotypic (morphology based on microscopic observations) and genotypic (16S rRNA and ITS sequences) analyses. The morphological identification shows that the strains belong to the orders Synechococcales, Oscillatoriales, Pleurocapsales, Chroococcidiopsidales and Nostocales. The strains are unicyanobacterial, but not axenic. They are available as living cultures, and the majority is also cryo-preserved (-70°C).

BCCM/ULC is progressively incorporating the most interesting strains from the research collection of the host Unit into the public collection. In addition, ongoing research and public deposits from other (more temperate) geographical areas enrich progressively the variety of proposed strains. The public collection now includes the type strain of the recently described species Plectolyngbya hodgsonii (Taton et al., 2011, Polar Biology, 34: 181-191).
Quality control description: The services of deposits and public distribution are certified by an ISO9001 certificate, since 2012.

Datasets

Dataset description
Object name: Darwin Core Archive BCCM/ULC Cyanobacteria culture collection at the University of Liège (ISO9001 certified)
Character encoding: UTF-8
Format name: Darwin Core Archive format
Format version: 1.0
Distribution: https://ipt.biodiversity.aq/archive.do?r=bccm_ulc_culture_collection_uliege
Publication date of data: 2020-11-08
Language: English
Licences of use: Creative Commons Attribution (CC-BY) 4.0 License

Metadata language: English
Date of metadata creation: 2019-01-14
Hierarchy level: Dataset

References
Taton, A., Grubisic, S., Ertz, D., Hodgson, D., Piccardi, R., Biondi, N., Tredici, M., Mainini, M., Losi, D., Marinelli, F., & Wilmotte, A. (2006). Polyphasic study of Antarctic cyanobacterial strains. Journal of Phycology, 42(6), 1257-1270. http://hdl.handle.net/2268/19904
Biondi, N., Tredici, M., Taton, A., Wilmotte, A., Hodgson, D., Losi, D., & Marinelli, F. (2008). Cyanobacteria from benthic mats of Antarctic lakes as a source of new bioactivities. Journal of Applied Microbiology, 105(1), 105-115. http://hdl.handle.net/2268/17380
A. Dumas, G. Laliberté, P. Lessard, J. de la Noüe, Biotreatment of fish farm effluents using the cyanobacterium Phormidium bohneri, Aquacultural Engineering, Volume 17, Issue 1, February 1998, Pages 57-68, ISSN 0144-8609, 10.1016/S0144-8609(97)01013-3.
P. Chevalier, D. Proulx, P. Lessard, W.F. Vincent and J. de la Noüe. (2000) Nitrogen and phosphorus removal by high latitude mat-forming cyanobacteria for potential use in tertiary wastewater treatment Journal of Applied Phycology Volume 12, Number 2, 105-112, DOI: 10.1023/A:1008168128654 - Talbot P. & De la Noue. J (1993) Tertiary treatment of wastewater with Phormidium bohneri (Schmidle) under various light and temperature conditions. Water Research, vol. 27, pp. 153-159
Nadeau T-L, Milbrandt EC & RC Castenholz (2001). Evolutionary relationships of cultivated Oscillatorians (Cyanobacteria). J. Phycol. 37: 650-654.
Strunecki, O, Komarek, J, Johansen J, Lukesova A & Elster J. 2013. Molecular and morphological criteria for revision of the genus Microcoleus (Oscillatoriales, Cyanobacteria). J. Phycol. 49, 1167-1180.
Jancusova, M.,  Kovacik, L.,  Pereira, A. B.,  Dusinsky, R., &   Wilmotte, A. (2016). Polyphasic characterization of 10 selected ecologically relevant filamentous cyanobacterial strains from the South Shetland Islands, Maritime Antarctica. FEMS Microbiology Ecology, 92 (7), 100
Sciuto K., Moschin E., Moro I. (2017) Cryptic cyanobacterial taxa from the Giant Cave (Triste Italy) Hydrobiologia;                               Katia Sciuto, Emanuela Moschin, and Isabella Moro (2017) Cryptic Cyanobacterial Diversity in the Giant Cave (Trieste, Italy): The New Genus Timaviella (Leptolyngbyaceae)  Cryptogamie, Algologie 38 (4), 285-323
Kopf M, Möke F, Bauwe H, Hess WR, Hagemann M. (2015). Expression profiling of the bloom-forming cyanobacterium Nodularia CCY9414 under light and oxidative stress conditions. ISME Journal 9: 2139–2152.