Heterotrophic bacterial diversity in aquatic microbial mat communities from Antarctica

Karolien Peeters1, Maxime Sweetlove2, Anne Willems1

1 Ghent University, Ghent, Belgium; 2 Royal Belgian Institute of Natural Sciences, Rue Vautier 29, 1000, Brussels

Corresponding author(s): Karolien Peeters, Maxime Sweetlove (msweetlove@naturalsciences.be)

Received {date}; Revised {date}; Accepted {date}; Published {date}

Citation: Combination of authors, year of data paper publication (in parentheses), Title, Journal Name, Volume, Issue number (in parentheses), and doi of the data paper.

Resource Citation

Peeters K (2020): Heterotrophic bacterial diversity in aquatic microbial mat communities from Antarctica. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Samplingevent. https://ipt.biodiversity.aq/resource?r=heterotrophic_bacterial_isolates_in_antarctica_microbial_mats&v=1.0

Abstract

This dataset describes heterotrophic bacteria that were isolated from five aquatic microbial mat samples from different locations in continental Antarctica and the Antarctic Peninsula.

Keywords: Samplingevent

Project details

Project title: Antarctic Microbial Biodiversity (AMBIO)
Personnel: Annick Wilmotte, Anne Willems, Elie Verleyen, Wim Vyverman
Funding: BelSPO SD/BA/01
Study area descriptions/descriptor: the Antarctic continent.

Data published through GBIF: https://ipt.biodiversity.aq/resource?r=heterotrophic_bacterial_isolates_in_antarctica_microbial_mats

Taxonomic coverage

General taxonomic coverage description: Heterotrophic Bacteria (504 strains) isolated from frozen microbial mat samples using culturing techniques.
Taxonomic ranks
Kingdom: Bacteria
Common names: Bacteria

Spatial coverage

General spatial coverage: Different water bodies (lakes) on the Antarctic continent: East Antarctica, the Trans-Antarctic Mountains, and the Antarctic Peninsula
Coordinates: 82°27'0''S and 67°42'0''S Latitude; 67°27'0''W and 39°47'60''E Longitude 

Natural collections description

Collection name: BCCM-LMG
Collection identifier: BCCM-LMG

Methods

Method step description: One gram of sample was aseptically weighed and homogenized in 9 ml sterile cold (4°C) physiological saline (0.86% NaCl) using a vortex. Tenfold dilution series (kept at 4°C) were plated on four different media (Marine agar 2216 (MA) (BD DifcoTM), R2A (BD DifcoTM), ten times diluted R2A (R2A/10), and PYGV (Pepton-Yeast-Glucose-Vitamin) medium (DSMZ medium 621)) and incubated at 20°C, 15°C and 4°C. R2A (Difco) contains pyruvate, starch and dextrose as C sources and yeast extract, peptone and casaminoacids as N and C sources and PYGV (DSMZ medium 621) contains peptone, yeast extract and glucose as C and/or N sources and additional vitamins and minerals. Both are considered oligotrophic media because the amounts of these components are at least two to ten times lower than in more general media such as nutrient broth. In addition to regular physiological saline (PS) dilution series, sea water (SW) dilutions were used for the LA3 and WO10 samples which originated from lakes close to the ocean and had elevated conductivity values.
Study extent description: Bacterial strain isolated from five aquatic microbial mat samples from different locations in continental Antarctica and the Antarctic Peninsula, sampled between 2003 and 2007.
Sampling description: Microbial mat samples were collected aseptically using a sterile spatula, and were kept frozen continuously after collection until processing in the laboratory. 

Datasets

Dataset description
Object name: Darwin Core Archive Heterotrophic bacterial diversity in aquatic microbial mat communities from Antarctica
Character encoding: UTF-8
Format name: Darwin Core Archive format
Format version: 1.0
Distribution: https://ipt.biodiversity.aq/archive.do?r=heterotrophic_bacterial_isolates_in_antarctica_microbial_mats
Publication date of data: 2020-01-16
Language: English
Licences of use: Creative Commons Attribution (CC-BY) 4.0 License

Metadata language: English
Date of metadata creation: 2020-01-16
Hierarchy level: Dataset

References
K. Peeters, E. Verleyen, D. A. Hodgson, P. Convey, D. Ertz , W. Vyverman, A. Willems (2012). Heterotrophic bacterial diversity in aquatic microbial mat communities from Antarctica. Polar Biol (2012) 35:543‐554. DOI 10.1007/s00300‐011‐1100‐4