Microorganisms (Bacteria, Archaea and phototroph eukaryotes) from Fildes Bay, King George Island, Antarctica

Mario Moreno-Pino1, Rodrigo De la Iglesia2, Nelson Valdivia3, Carlos Hendriquez-Castilo2, Alexander Galan4, Beatriz Diez5, Nicole Trefault1, Maxime Sweetlove6

1 Universidad Mayor, Santiago, Chile; 2 Pontificia Universidad Catolica de Chile, Santiago, Chile; 3 Universidad Australe de Chile, Valdivia, Chile; 4 Universidad Catolica de la Santisima Concepcion, Talcahuano, Chile; 5 Universidad Catolica de Chile, Santiago, Chile; 6 Royal Belgian Institute of Natural Sciences, Rue Vautier 29, 1000, Brussels, Belgium

Corresponding author(s): Mario Moreno-PinoRodrigo De la IglesiaNelson ValdiviaCarlos Hendriquez-CastiloAlexander GalanBeatriz DiezNicole Trefault, Maxime Sweetlove (msweetlove@naturalsciences.be)

Received {date}; Revised {date}; Accepted {date}; Published {date}

Citation: Combination of authors, year of data paper publication (in parentheses), Title, Journal Name, Volume, Issue number (in parentheses), and doi of the data paper.

Resource Citation

Moreno-Pino M, De la Iglesia R, Valdivia N, Hendriquez-Castilo C, Galan A, Diez B, Trefault N (2019): Microorganisms (Bacteria, Archaea and phototroph eukaryotes) from Fildes Bay, King George Island, Antarctica. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=microbes_fildes_bay_antarctica&v=1.1

Abstract

Amplicon sequencing dataset (Illumina MiSeq and 454 pyrosequencing) of Bacteria and Archaea (16S ssu rRNA gene) and phototroph eukaryotes (16S chloroplast) from sea water in Fildes Bay, King George Island, Antarctica

Keywords: Metadata

Data published through GBIF: https://ipt.biodiversity.aq/resource?r=microbes_fildes_bay_antarctica

Taxonomic coverage

General taxonomic coverage description: Bacteria and Archaea (16S ssu rRNA gene)
Taxonomic ranks
Domain: Bacteria, Archaea
Common names: Bacteria, Archaea
Taxonomic coverage

General taxonomic coverage description: phototrophic eukaryotes, based on the chloroplast 16S ssu rRNA gene
Taxonomic ranks
Domain: Eukaryota
Common names: algae

Spatial coverage

General spatial coverage: Fildes Bay, King George Island, Antarctica
Coordinates: 62°17'24''S and 62°9'36''S Latitude; 58°56'24''W and 58°43'48''W Longitude 

Methods

Method step description: 
- . 	For photosynthetic eukaryote identification, plastidial 16S rRNA PCR products (in triplicates) were obtained using primer pair PLA491F–OXY1313, purified using Zymoclean kit (Zymo Research) and checked on an Agilent Bioanalzyer DNA1000 chip for the absence of primer dimers, and quantified using a PicoGreen dsDNA quantitation reagent (Invitrogen). Equal amount of purified PCR products were pooled for subsequent 454 pyrosequencing using a Roche GS-FLX Junior.
- . 	For bacterial identification, general 16S rRNA PCR products (in triplicates) were obtained using primers 515Fseq and 806rcbc (Caporaso et al.2011) following conditions from Earth Microbiome Project (EMP) (Gilbert, Jansson and Knight 2014). Illumina primer constructs were obtained from EMP also. Amplicons were quantified using KAPA Library Quantification Kit (KAPA Biosystem) and sequenced using Illumina Miseq following Caporaso et al. (2012) protocol. 12 pM of qPCR quantified amplicons pool were sequenced using a 300 cycles Illumina Miseq kit.
Study extent description: Water samples were taken at Fildes Bay, King George Island, Antarctica, on 7 and 8 February 2012
Sampling description: Surface water samples (5 m depth) were collected with 5 L Niskin bottles from 17 different locations. Each location was assigned to one of the following four categories: Collins Glacier (‘C’ stations), Nelson Glacier (‘N’ stations), Fildes Bay (‘F’ stations) and Inner Bay (‘IB’ stations). Seawater samples (5 L) were prefiltered on board through 100 μm pore mesh and stored in acid-washed carboys and kept on dark until subsampling at the laboratory.

Metadata language: English
Date of metadata creation: 2019-02-15
Hierarchy level: Dataset

References
Moreno-Pino, M., De la Iglesia, R., Valdivia, N., Henríquez-Castilo, C., Galán, A., Díez, B., & Trefault, N. (2016). Variation in coastal Antarctic microbial community composition at sub-mesoscale: spatial distance or environmental filtering?. FEMS microbiology ecology, 92(7). doi: 10.1093/femsec/ w088