Microbial metabarcoding surveys (Bacteria, Archaea and Eukaryota) of the arctic marine environment

Eric Collins1, Maxime Sweetlove2

1 College of Fisheries and Ocean Sciences , Fairbanks, United States; 2 Royal Belgian Institute of Natural Sciences, Rue Vautier 29, 1000, Brussels, Belgium

Corresponding author(s): Eric Collins (recollins@alaska.edu), Maxime Sweetlove (msweetlove@naturalsciences.be)

Received {date}; Revised {date}; Accepted {date}; Published {date}

Citation: Combination of authors, year of data paper publication (in parentheses), Title, Journal Name, Volume, Issue number (in parentheses), and doi of the data paper.

Abstract

Large amplicon sequencing dataset (Illumina MiSeq) targeting Bacteria/Archaea (16S ssu rRNA gene) and Eukaryota (18S ssu rRNA gene) from samples taken in the Arctic marine environment, following the Earth Microbiome Project protocol.

Keywords: Metadata

Data published through GBIF: https://ipt.biodiversity.aq/resource?r=microbial_metabarcoding_surveys_arctic_marine_environment

Taxonomic coverage

General taxonomic coverage description: Eukaryotes in marine environments, profiled by sequencing the v4 region of the 18S ssu rRNA gene, using the primer set published by Stoeck et al. (doi:10.1111/j.1365-294X.2009.04480.x)
TAReuk454FWD1 CCAGCASCYGCGGTAATTCC
TAReukREV3_modified ACTTTCGTTCTTGATYRATGA
Taxonomic ranks
Domain: Eukaryota
Common names: Eukaryotes
Taxonomic coverage

General taxonomic coverage description: Bacteria and Archaea in marine environments, profiled by sequencing the v4 region of the 16S ssu rRNA gene, using the primer set published by Apprill et al. (doi:10.3354/ame01753)
515F GTGCCAGCMGCCGCGGTAA
806RB GGACTACNVGGGTWTCTAAT
Taxonomic ranks
Domain: Bacteria, Archaea
Common names: Bacteria, Archaea

Spatial coverage

General spatial coverage: Complete coverage of the Arctic marine environments.
Coordinates: 67°40'4.8''N and 89°59'25.2''N Latitude; 176°45'41.16''W and 157°49'39.42''E Longitude 

Temporal coverage: November 20, 2007 - July 29, 2016

Metadata language: English
Date of metadata creation: 2019-03-28
Hierarchy level: Dataset