Western Antarctic Peninsula Bacteria (Mesocosm exp.)

Catherine Luria1, Linda Amaral-Zettler2, Hugh Ducklow3, Daniel Repeta4, Andrew Rhyne5, Jeremy Rich6, Maxime Sweetlove7

1 Brown University, , United States; 2 Royal Netherlands Institute for Sea Research, , Netherlands; 3 Lamont-Doherty Earth Observatory, , United States; 4 Woods Hole Oceanographic Institution, Woods Hole, United States; 5 Roger Williams University, Bristol, United States; 6 University of Maine, Walpole, United States; 7 Royal Belgian Institude of Natural Sciences, Rue Vautier 29, 1000, Brussels, Belgium

Corresponding author(s): Catherine LuriaLinda Amaral-ZettlerHugh DucklowDaniel RepetaAndrew RhyneJeremy Rich, Maxime Sweetlove (msweetlove@naturalsciences.be)

Received {date}; Revised {date}; Accepted {date}; Published {date}

Citation: Combination of authors, year of data paper publication (in parentheses), Title, Journal Name, Volume, Issue number (in parentheses), and doi of the data paper.

Resource Citation

Luria C, Amaral-Zettler L, Ducklow H, Repeta D, Rhyne A, Rich J (2018): Western Antarctic Peninsula Bacteria (Mesocosm exp.). v1.4. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=western_peninsula_microbes&v=1.4

Abstract

Amplicon sequencing data of the 16S SSU rRNA marker gene of bacteria in four Mesososm experiments that spanned the spring transitional period (August–December 2013) in surface waters of the Western Antarctic Peninsula (WAP). Each mesocosm consisted of nearshore surface seawater (50 L) incubated in the laboratory for 10 days. 

Further details in: Luria, C. M., Amaral-Zettler, L. A., Ducklow, H. W., Repeta, D. J., Rhyne, A. L., & Rich, J. J. (2017). Seasonal shifts in bacterial community responses to phytoplankton-derived dissolved organic matter in the Western Antarctic Peninsula. Frontiers in microbiology, 8, 2117.

Keywords: Metadata

Project details

Project title: Western Antarctic Peninsula Bacteria (Mesocosm exp.)
Personnel: Catherine Lucia, Linda Amaral-Zettler, Hugh Ducklow, Daniel Repeta, Andrew Rhyne, Jeremy Rich
Funding: CL was partially funded by the Graduate School and the Department of Ecology and Evolutionary Biology at Brown University and the Brown University-Marine Biological Laboratory Joint Graduate Program. This material is based upon work supported by the National Science Foundation under Grant Nos. ANT-1142114 to LA-Z, OPP-0823101 and PLR-1440435 to HD, and ANT-1141993 to JR. The Gordon and Betty Moore Foundation grant 1711 supported work by DR.
Study area descriptions/descriptor: Western Antarctic Peninsula
Design description: Mesocosm experiments were conducted in August, September, October, and December 2013 at Palmer Station by filling acid-washed 50 L polycarbonate carboys with seawater from the station’s intake. The filled carboys were divided into two treatments: (1) control (no additions, n = 3) and (2) +DOM (carboys that received diatom exudates; n = 3). An additional f/2 control treatment (uninoculated growth media, n = 3) was included in the October experiment only. The DOC concentration in the f/2 control was not any different than controls without any added DOC. As such, the f/2 control served as an additional no DOC addition control. The mesocosms were incubated for 10 days at 0°C, except in the August experiment when mesocosms were incubated at 3°C. All experiments were conducted under a low-level of continuous light, 1 × 1014 quanta cm-2 s-1, as measured inside the carboys with a hand-held radiometer (Biospherical Instruments, Inc.).

Data published through GBIF: https://ipt.biodiversity.aq/resource?r=western_peninsula_microbes

Taxonomic coverage

General taxonomic coverage description: Bacteria (16S ssu rRNA gene, v6 region)
Taxonomic ranks
Domain: Bacteria
Common names: Bacteria

Spatial coverage

General spatial coverage: Sea off the Western Antarctic Peninsula
Coordinates: 64°46'12''S and 64°46'12''S Latitude; 64°10'48''W and 64°2'60''W Longitude 

Formation period: 2009-2014

Methods

Method step description: Mesocosms were set up at Palmer Station, Antarctica, using seawater from the station’s intake, located at a depth of 6 m, 16 m from the shore of the station. This source water was monitored during the experimental period (August–December 2013) to determine any changes in environmental parameters that might influence bacterial community responses in mesocosm experiments. Samples for dissolved nutrients (phosphate, silicate, nitrite, and nitrate) and particulate organic carbon and nitrogen (POC and PN) were processed according to Palmer LTER standard protocols
Study extent description: Western Antarctic Peninsula
Sampling description: Mesocosms were set up at Palmer Station, Antarctica, using seawater from the station’s intake, located at a depth of 6 m, 16 m from the shore of the station. This source water was monitored during the experimental period (August–December 2013) to determine any changes in environmental parameters that might influence bacterial community responses in mesocosm experiments. Samples for dissolved nutrients (phosphate, silicate, nitrite, and nitrate) and particulate organic carbon and nitrogen (POC and PN) were processed according to Palmer LTER standard protocols

Metadata language: English
Date of metadata creation: 2018-11-08
Hierarchy level: Dataset

References
Luria, C. M., Amaral-Zettler, L. A., Ducklow, H. W., Repeta, D. J., Rhyne, A. L., & Rich, J. J. (2017). Seasonal shifts in bacterial community responses to phytoplankton-derived dissolved organic matter in the Western Antarctic Peninsula. Frontiers in microbiology, 8, 2117.