Microbial (Bacteria, 16S) Diversity in Antarctic marine sediments (Admiralty Bay and Bransfield Strait)

Última versión Publicado por SCAR - Microbial Antarctic Resource System en Mar 19, 2019 SCAR - Microbial Antarctic Resource System

amplicon dataset of Bacteria (16S ssh rRNA marker gene) found Antarctic marine sediments (Admiralty Bay and Bransfield Strait), sampled in December 2008.

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Versiones

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¿Cómo referenciar?

Los usuarios deben citar este trabajo de la siguiente manera:

Franco D, Signori C, Duarte R, Nakayama C, Campos L, Pellizari V (2018): Microbial (Bacteria, 16S) Diversity in Antarctic marine sediments (Admiralty Bay and Bransfield Strait). v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=antarctic_marine_sediment_microbes&v=1.2

Derechos

Los usuarios deben respetar los siguientes derechos de uso:

El publicador y propietario de los derechos de este trabajo es SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

Registro GBIF

Este recurso ha sido registrado en GBIF con el siguiente UUID: 2afaca55-5c1e-456e-be07-53fc268035d7.  SCAR - Microbial Antarctic Resource System publica este recurso, y está registrado en GBIF como un publicador de datos avalado por Scientific Committee on Antarctic Research.

Palabras Clave

Metadata

Contactos

¿Quién creó el recurso?:

Diego Franco
Universidade de São Paulo São Paulo BR
Camila Signori
Universidade de São Paulo São Paulo BR
Rubens Duarte
Universidade Federal de Santa Catarina Florianópolis BR
Cristina Nakayama
Universidade Federal de São Paulo Diadema
Lucia Campos
Universidade Federal do Rio de Janeiro Rio de Janeiro BR
Vivian Pellizari
Universidade de São Paulo São Paulo BR

¿Quién puede resolver dudas acerca del recurso?:

Diego Franco
Universidade de São Paulo São Paulo BR

¿Quién documentó los metadatos?:

Maxime Sweetlove
Research assistent
Royal Belgian Institute for Natural Sciences Rue Vautier 29 1000 Brussels

¿Quién más está asociado con el recurso?:

Usuario

Cobertura Geográfica

King George Island, Antarctica

Coordenadas límite Latitud Mínima Longitud Mínima [-62.293, -58.476], Latitud Máxima Longitud Máxima [-62.08, -58.17]

Cobertura Taxonómica

Bacteria 16S ssh rRNA

Dominio  Bacteria (Bacteria)

Cobertura Temporal

Fecha Inicial / Fecha Final 2008-12-01 / 2008-12-06

Datos del Proyecto

No hay descripción disponible

Título Microbial Diversity in Antarctic marine sediments
Fuentes de Financiación This dataset was funded by the Brazilian Antarctic Program (PROANTAR), and the Brazilian National Council for Scientific and Technological Development – CNPq (MABIREH/IPY/CAML Project n. 520293/2006-1).

Personas asociadas al proyecto:

Diego Franco

Métodos de Muestreo

In total, 15 samples of marine sediments were collected using a Mini Box Corer (MBC) at different depths. The top 5 cm of sediments were transferred to sterile Whirl-Pack sample bags (Nasco, WI, USA) and stored frozen onboard (-20°C). Samples were shipped to the University of São Paulo (USP) after 4 months of sampling.

Área de Estudio Surface sediment samples were collected along a bathymetric gradient in King George Island (stations 1–9, depths ranging from 100 to 502 m) and NBB – Bransfield Strait (stations 10–15, depths 693–1,147 m), located in the Northwestern Antarctic Peninsula. Sampling was conducted by the Brazilian Navy vessel NApOc Ary Rongel during the austral summer, December 2008.

Descripción de la metodología paso a paso:

  1. Genomic DNA was extracted from 0.25 g of surface sediment in quadruplicate using a PowerSoil DNA Kit (MoBio, Carlsbad, CA, USA), according to the manufacturer’s instructions. Microbial 16S rRNA gene fragments were amplified using a set of primers designed by adding a 10-nucleotide barcode to the forward primer, 519F, (5′-CAGCMGCCGCGGTAATWC-3′) and reverse primer 1068R (5′-CTGACGRCRGCCATGC-3′). The amplification reaction was carried out using the Accuprime pfx SuperMix (Thermo Scientific, USA) according to the manufacturer. PCR was performed with a thermal cycler (Thermo Scientific, USA) under the following conditions: 95°C for 5 min, 26 cycles of 95°C for 15 s, 59°C for 30 s and 68°C for 1 min. The PCR products were purified by using a DNA clean & concentrator kit (Zymo Research, USA).
  2. The amplicons from each sample were mixed at equimolar concentrations and then sequenced using GSFLX titanium instruments and reagents (Roche 454, Life Sciences, USA) at the Center for Advanced Technologies in Genomics (University of São Paulo, Brazil).

Referencias Bibliográficas

  1. Franco, D. C., Signori, C. N., Duarte, R. T., Nakayama, C. R., Campos, L. S., & Pellizari, V. H. (2017). High prevalence of gammaproteobacteria in the sediments of admiralty bay and north bransfield Basin, Northwestern Antarctic Peninsula. Frontiers in microbiology, 8, 153.

Metadatos Adicionales

Identificadores Alternativos 2afaca55-5c1e-456e-be07-53fc268035d7
https://ipt.biodiversity.aq/resource?r=antarctic_marine_sediment_microbes