Antarctic pack ice Bacterial (16S) communities

最新バージョン SCAR - Microbial Antarctic Resource System によって公開 Mar 19, 2019 SCAR - Microbial Antarctic Resource System

Amplicon sequencing dataset of Bacterial communities (16S ssu rRNA gene) in pack ice from the Southern Ocean, near Antarctica.

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バージョン

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引用方法

研究者はこの研究内容を以下のように引用する必要があります。:

Eronen-Rasimus E, Luhtanen A, Rintala J, Delille B, Dieckmann G, Karkman A, Tison J (2018): Antarctic pack ice Bacterial (16S) communities. v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=antarctic_pack_ice_bacterial_communities&v=1.2

権利

研究者は権利に関する下記ステートメントを尊重する必要があります。:

パブリッシャーとライセンス保持者権利者は SCAR - Microbial Antarctic Resource System。 This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF登録

このリソースをはGBIF と登録されており GBIF UUID: bd9de0e6-69c1-4ec6-b49b-fa6f84454163が割り当てられています。   Scientific Committee on Antarctic Research によって承認されたデータ パブリッシャーとして GBIF に登録されているSCAR - Microbial Antarctic Resource System が、このリソースをパブリッシュしました。

キーワード

Metadata

連絡先

リソースを作成した人:

Eeva Eronen-Rasimus
University of Helsink Helsink FI
Anne-Mari Luhtanen
University of Helsink Helsink FI
Janne-Markus Rintala
University of Helsink Helsink FI
Bruno Delille
Université de Liège Liège BE
Gerhard Dieckmann
Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Bremerhaven DE
Antti Karkman
University of Helsink Helsink FI
Jean-Louis Tison
Université Libre de Bruxelles Campus du Solbosch CP160/03, avenue F.D. Roosevelt 50 1050 Brussels

リソースに関する質問に答えることができる人:

Eeva Eronen-Rasimus
University of Helsink Helsink FI

メタデータを記載した人:

Maxime Sweetlove
Research assistent
Royal Belgian Istitute for Natural Sciences Rue Vautier 29 1000 Brussels

他に、リソースに関連付けられていた人:

データ利用者

地理的範囲

Antarctica: Southern Ocean: Wedell Sea

座標(緯度経度) 南 西 [-67.949, -0.006], 北 東 [-61.526, 0.122]

生物分類学的範囲

Bacteria 16S ssu rRNA marker gene, v1-v3

Domain  Bacteria (Bacteria)

時間的範囲

開始日 / 終了日 2013-06-21 / 2013-07-26

プロジェクトデータ

説明がありません

タイトル Antarctic pack ice Bacterial (16S) communities
ファンデイング The creation of this dataset was supported by the Walter and Andrée de Nottbeck Foundation, Academy of Finland and the Belgian Science Policy (Bigsouth project, SD/CA/05).

プロジェクトに携わる要員:

Eeva Eronen-Rasimus

収集方法

The ice cores were drilled with a trace-metal-clean (electropolished steel) ice auger, 14 cm in diameter. Two ice cores were collected and pooled at each station for the microbiological analyses. We emphasised careful sampling and subsequent sample processing to avoid contamination. The ice cores collected were cut with an ethanol-wiped handsaw into two to seven pieces, depending on the ice thickness (each horizon 10–30 cm), crushed and placed in sterile plastic containers at 4 °C over night in darkness after which the rest of the ice was quickly melted in a water bath with constant stirring. The melted samples were immediately filtered after becoming fully melted.

Study Extent The samples were collected from 10 pack-ice stations along the Weddell and Lazarev Seas during the Antarctic Winter Ecosystem Climate Study (AWECS, leg ANT-XXIX/6)-expedition aboard the R/V Polarstern during the austral winter in June–July 2013.

Method step description:

  1. For the DNA extractions, approximately 500 ml of the melted sea-ice were filtered onto sterile 0.22-μm membrane filters (Ø 47 mm; Whatman GE Healthcare, Little Chalfont, Kent, UK) and frozen in liquid nitrogen and later transferred to –80 °C.
  2. The bacterial community DNA was extracted from the filters with a PowerSoil DNA Isolation Kit (Mo Bio Laboratories Inc, Carlsbad, CA, USA), as described by Eronen-Rasimus et al. (2014), 6 months after the cruise. In addition to the samples, negative controls without the sample were extracted.
  3. The 16S ribosomal RNA gene region from V1 to part of the V3 was amplified with a polymerase chain reaction, using the universal bacterial primers F8 and R492. A two-step polymerase chain reaction and Illumina MiSeq (Illumina Inc, San Diego, CA, USA) paired-end multiplex sequencing were performed at the Institute of Biotechnology, University of Helsinki, Finland.

書誌情報の引用

  1. Eronen-Rasimus, E., Luhtanen, A. M., Rintala, J. M., Delille, B., Dieckmann, G., Karkman, A., & Tison, J. L. (2017). An active bacterial community linked to high chl-a concentrations in Antarctic winter-pack ice and evidence for the development of an anaerobic sea-ice bacterial community. The ISME Journal, 11(10), 2345.

追加のメタデータ

代替識別子 bd9de0e6-69c1-4ec6-b49b-fa6f84454163
https://ipt.biodiversity.aq/resource?r=antarctic_pack_ice_bacterial_communities